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AB211084

Nitric Oxide Synthase Activity Assay Kit (Fluorometric)

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(1 Review)

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(4 Publications)

Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084) provides an accurate and convenient method to assay nitric oxide synthase (NOS) activity in cell and tissue lysates.
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Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (AB211084)
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Supplier Data

Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (AB211084)

Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084). Typical standard calibration curve.

Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (AB211084)
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Supplier Data

Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (AB211084)

Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084). Detection of endogenous NOS activity in J774.1A cell lysates. J774 cells were stimulated with 200 ng/mL LPS and 100 ng/mL murine IFN-gamma. Unstimulated control included. Cell lysates were prepared (225 μg) and assayed following the kit protocol.

Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (AB211084)
  • FuncS

Supplier Data

Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (AB211084)

Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084). Detection of endogenous NOS activity in cell lysates from J774.1A mouse monocytes (135 μg) and HeLa cells (157 μg).

Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (AB211084)
  • FuncS

Supplier Data

Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (AB211084)

Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084). Measurement of NOS Positive Control activity (5 μL) compared to blank (assay buffer).

Key facts

Detection method

Fluorescent

Sample types

Tissue Lysate, Cell Lysate

Assay type

Enzyme activity

Results type

Quantitative

Sensitivity

= 0.75 µU

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084) provides an accurate and convenient method to assay nitric oxide synthase (NOS) activity in cell and tissue lysates. In this assay, nitric oxide (NO) generated by NOS undergoes a series of reactions and reacts with the fluorescent probe to generate a stable signal at Ex/Em = 360/450 nm, which is directly proportional to NOS activity. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 0.75 μU of NOS activity.

This product is manufactured by BioVision, an Abcam company and was previously called K206 Nitric Oxide Synthase (NOS) Activity Assay Kit (Fluorometric). K206-100 is the same size as the 100 test size of ab211084.

Nitric oxide synthases (EC 1.14.13.39) (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body's defense mechanism.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.
Biological function summary

NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.

Pathways

NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.

NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.
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Product protocols

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Target data

See full target information NOS
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 9: PubMed34944662

2021

Activation of PPARα by Fenofibrate Attenuates the Effect of Local Heart High Dose Irradiation on the Mouse Cardiac Proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Omid Azimzadeh,Vikram Subramanian,Wolfgang Sievert,Juliane Merl-Pham,Kateryna Oleksenko,Michael Rosemann,Gabriele Multhoff,Michael J Atkinson,Soile Tapio

Folia neuropathologica 58:253-264 PubMed33099295

2020

Inhibition of MAPT enhances the effect of bexarotene and attenuates the damage after traumatic brain injury using in vivo and in vitro experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Haihai Dong,Haitao Wang,Liang Wang

European journal of nutrition 59:3669-3689 PubMed32067099

2020

Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Tanvi Shinde,Agampodi Promoda Perera,Ravichandra Vemuri,Shakuntla V Gondalia,David J Beale,Avinash V Karpe,Sonia Shastri,Waheedha Basheer,Benjamin Southam,Rajaraman Eri,Roger Stanley

Nutrients 11: PubMed30979002

2019

Synbiotic Supplementation Containing Whole Plant Sugar Cane Fibre and Probiotic Spores Potentiates Protective Synergistic Effects in Mouse Model of IBD.

Applications

Unspecified application

Species

Unspecified reactive species

Tanvi Shinde,Agampodi Promoda Perera,Ravichandra Vemuri,Shakuntla V Gondalia,Avinash V Karpe,David J Beale,Sonia Shastri,Benjamin Southam,Rajaraman Eri,Roger Stanley
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Product promise

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