Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084) provides an accurate and convenient method to assay nitric oxide synthase (NOS) activity in cell and tissue lysates.
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- Biomedicines 9:2021Activation of PPARα by Fenofibrate Attenuates the Effect of Local Heart High Dose Irradiation on the Mouse Cardiac Proteome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOmid Azimzadeh,Vikram Subramanian,Wolfgang Sievert,Juliane Merl-Pham,Kateryna Oleksenko,Michael Rosemann,Gabriele Multhoff,Michael J Atkinson,Soile TapioPubMed 34944662
- Folia neuropathologica 58:253-2642020Inhibition of MAPT enhances the effect of bexarotene and attenuates the damage after traumatic brain injury using in vivo and in vitro experiments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaihai Dong,Haitao Wang,Liang WangPubMed 33099295
- European journal of nutrition 59:3669-36892020Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTanvi Shinde et. al.PubMed 32067099
- Nutrients 11:2019Synbiotic Supplementation Containing Whole Plant Sugar Cane Fibre and Probiotic Spores Potentiates Protective Synergistic Effects in Mouse Model of IBD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTanvi Shinde et. al.PubMed 30979002
Key facts
- Detection method
Fluorescent
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Sensitivity
= 0.75 µU
Target data
What's included?
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Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084) provides an accurate and convenient method to assay nitric oxide synthase (NOS) activity in cell and tissue lysates.
Key facts
- Detection method
Fluorescent
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Assay Platform
Microplate reader
- Sensitivity
= 0.75 µU
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Storage information
Please refer to protocols
Notes
Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084) provides an accurate and convenient method to assay nitric oxide synthase (NOS) activity in cell and tissue lysates. In this assay, nitric oxide (NO) generated by NOS undergoes a series of reactions and reacts with the fluorescent probe to generate a stable signal at Ex/Em = 360/450 nm, which is directly proportional to NOS activity. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 0.75 μU of NOS activity.
This product is manufactured by BioVision, an Abcam company and was previously called K206 Nitric Oxide Synthase (NOS) Activity Assay Kit (Fluorometric). K206-100 is the same size as the 100 test size of ab211084.
Nitric oxide synthases (EC 1.14.13.39) (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body's defense mechanism.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NNOS also known as nitric oxide synthase 1 or NOS1 is a critical enzyme in the nervous system. It catalyzes the production of nitric oxide from L-arginine. This enzyme weighing approximately 160 kDa is expressed in neurons and you can find it in regions like the brain and spinal cord. Notably nNOS is part of the NOS family which also includes eNOS and iNOS but its primary function is in neuronal tissues.
- Biological function summary
NNOS plays a significant role in neurotransmission and neurovascular regulation. As a part of protein complexes nNOS closely interacts with other signaling molecules to modulate neuronal communication. The production of nitric oxide by nNOS acts as a neurotransmitter influencing brain functions such as synaptic plasticity and memory formation. Additionally due to its role in neuronal activity antibodies like anti-neuronal antibodies can have interactions that can be studied using various assays including the NOS assay.
- Pathways
NNOS serves essential roles within the neuronal nitric oxide signaling pathway and the glutamatergic signaling pathway. In these contexts nNOS associates with proteins involved in cell signaling such as calmodulin which regulates its activity. By participating in these pathways nNOS contributes to the regulation of vascular tone and synaptic transmission linking it to neurological processes and supporting neuronal communication.
- Associated diseases and disorders
NNOS shows significant connections to neurological disorders and cardiovascular diseases. In conditions like stroke and neurodegenerative diseases dysregulation of nNOS activity leads to excessive production of nitric oxide which can result in oxidative stress and neuronal damage. Furthermore its relation with eNOS in the cardiovascular system influences vascular function making it a critical player in hypertension studies. Understanding nNOS interactions through diseases highlights the possibility of targeted treatments using neuronal antibodies and anti-neuronal antibodies against specific pathways.
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4 product images
Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084)
Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084). Typical standard calibration curve.
Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084)
Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084). Detection of endogenous NOS activity in cell lysates from J774.1A mouse monocytes (135 μg) and HeLa cells (157 μg).
Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084)
Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084). Detection of endogenous NOS activity in J774.1A cell lysates. J774 cells were stimulated with 200 ng/mL LPS and 100 ng/mL murine IFN-gamma. Unstimulated control included. Cell lysates were prepared (225 μg) and assayed following the kit protocol.
Functional Studies - Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084)
Nitric Oxide Synthase Activity Assay Kit (Fluorometric) (ab211084). Measurement of NOS Positive Control activity (5 μL) compared to blank (assay buffer).
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