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AB324120

Nitroreductase Assay Kit (Luminometric)

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Nitroreductase Assay Kit ab324120 is based on a luciferin derivative that is acted on by nitroreductases. Readout on luminescence plate reader.
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Functional Studies - Nitroreductase Assay Kit (Luminometric) (AB324120)
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Supplier Data

Functional Studies - Nitroreductase Assay Kit (Luminometric) (AB324120)

Nitroreductase dose response was measured in a white solid 96-well plate with Nitroreductase Assay Kit (Luminometric) using a NOVO star plate reader (BMG Labtech). The kit can detect as low as 20 ng/mL of NTR (The data was obtained about 5 min after adding the NTR detection working solution).

Key facts

Assay type

Enzyme activity

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Product details

Nitroreductase Assay Kit (Luminometric) (ab324120) provides a highly sensitive and convenient method to quantify nitroreductase activity.

Nitroreductase assay principle
The nitroreductase assay uses a luciferin derivative that can be selectively reduced by nitroreductase. The amount of generated luciferin is then detected using a luciferase assay system.The luminescent intensity generated is proportional to the nitroreductase activity.

The assay can detect as low as 20 ng/mL of nitroreductase.

Nitroreductase assay protocol summary
- add samples and standard to wells- add first working solution and incubate for 60-min at 37°C- add second working solution and monitor luminescence in plate reader

Other Notes
Nitroreductases are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds. They are absent in mammalian cells but widespread in bacteria. Nitroreductases play a crucial role in the reduction of nitroaromatic compounds via NAD(P)H-dependent reactions. Nitroreductases are also targets for developing novel antibiotics, degradation of pollutants, and cancer therapy.

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What's included?

{ "values": { "100Tests": { "sellingSize": "100 Tests", "publicAssetCode":"ab324120-100Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"NTR Reaction Buffer", "number":"AB324120-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Nitroreductase Standard", "number":"AB324120-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NTR Substrate", "number":"AB324120-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"NTR Detection Buffer", "number":"AB324120-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NTR Reaction Enzyme", "number":"AB324120-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NTR Detection Mix 2", "number":"AB324120-CMP07", "productcode":"" }, { "size":"1 x 100 µL", "name":"DMSO", "number":"AB324120-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NTR Detection Mix 1", "number":"AB324120-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information Nitroreductase
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websiteProtocolBooklet
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Product promise

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