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NQO1 Activity Assay kit (ab184867) is designed for the sensitive and accurate measurement of NQO1 (NAD(P)H dehydrogenase [quinone] 1) activity.

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Detection method

Colorimetric

Sample types

Tissue Lysate, Cell culture extracts, Suspension cells, Tissue Extracts, Tissue Homogenate, Adherent cells, Cell Lysate

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Cow, Human

Assay time

30m

Images

Target data

What's included?

1 x 96 Tests
Components
1000X Cofactor (lyophilized)
1 x 1 Vial
1000X Inhibitor (lyophilized)
1 x 1 Vial
100X Dye (lyophilized)
1 x 1 Vial
20X Basic Buffer
1 x 3 mL
2X Extraction Buffer
1 x 15 mL
5000X Menadione
1 x 100 µL
500X NADH (lyophilized)
1 x 1 Vial
96-well Microplate
2 x 1 Unit

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NQO1 Activity Assay kit (ab184867) is designed for the sensitive and accurate measurement of NQO1 (NAD(P)H dehydrogenase [quinone] 1) activity.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell culture extracts, Suspension cells, Tissue Extracts, Tissue Homogenate, Adherent cells, Cell Lysate

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Cow, Human

Assay time

30m

Assay Platform

Microplate reader

Precision

Intra assay

Sample
Overall
n
3
mean
-
SD
-
C.V.
2.183

Inter assay

Sample
Overall
n
9
mean
-
SD
-
C.V.
6.035

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

NQO1 Activity Assay kit (ab184867) is designed for the sensitive and accurate measurement of NQO1 (NAD(P)H dehydrogenase [quinone] 1) activity.

The enzyme activity is determined by following the reduction of Menadione with cofactor NADH and the simultaneous reduction of WST1 which leads to increased absorbance at 440 nm. Dicoumarol is used as an inhibitor of NQO1 as part of the assay.

NQO1 assay protocol summary:
- add samples to wells
- add reaction buffer + inhibitor and reaction buffer to separate sample wells
- analyze with a microplate reader for 5 min

NQO1 serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways, NQO1 protects against quinone-induced damage by competing with potentially toxic one-electron pathways. It also functions in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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