NQO1 Activity Assay kit (ab184867) is designed for the sensitive and accurate measurement of NQO1 (NAD(P)H dehydrogenase [quinone] 1) activity.
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- Current research in toxicology 5:1001262023Methylmercury (MeHg) transcriptionally regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) in Hepa-1c1c7 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammed A Alqahtani,Mahmoud A El-Ghiaty,Sara R El-Mahrouk,Ayman O S El-KadiPubMed 37808441
- Toxins 15:2023Discovering the Protective Effects of Quercetin on Aflatoxin B1-Induced Toxicity in Bovine Foetal Hepatocyte-Derived Cells (BFH12).Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarianna Pauletto,Mery Giantin,Roberta Tolosi,Irene Bassan,Anisa Bardhi,Andrea Barbarossa,Ludovica Montanucci,Anna Zaghini,Mauro DacastoPubMed 37755981
- Acta pharmacologica Sinica 44:1429-14412023Skullcapflavone II, a novel NQO1 inhibitor, alleviates aristolochic acid I-induced liver and kidney injury in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYa-Ping Dong,Shu-Zhen Chen,Hui-Si He,Zhuo-Ran Sun,Li-Xuan Jiang,Yan-Qiu Gu,Ying Zhang,Fei Feng,Chun Chen,Zhe-Cai Fan,Xiao-Fei Chen,Wen Wen,Hong-Yang WangPubMed 36697978
- Pharmaceutics 14:2022Arene Variation of Highly Cytotoxic Tridentate Naphthoquinone-Based Ruthenium(II) Complexes and In-Depth In Vitro Studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKlaudia Cseh,Heiko Geisler,Kristina Stanojkovska,Julia Westermayr,Philipp Brunmayr,Dominik Wenisch,Natalie Gajic,Michaela Hejl,Martin Schaier,Gunda Koellensperger,Michael A Jakupec,Philipp Marquetand,Wolfgang KandiollerPubMed 36432656
- Toxins 14:2022Deepening the Whole Transcriptomics of Bovine Liver Cells Exposed to AFB1: A Spotlight on Toll-like Receptor 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvia Iori,Marianna Pauletto,Irene Bassan,Federico Bonsembiante,Maria Elena Gelain,Anisa Bardhi,Andrea Barbarossa,Anna Zaghini,Mauro Dacasto,Mery GiantinPubMed 35878242
- Marine drugs 20:2021Fucoxanthin Pretreatment Ameliorates Visible Light-Induced Phagocytosis Disruption of RPE Cells under a Lipid-Rich Environment via the Nrf2 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunjun Liu,Zixin Guo,Shengnan Wang,Yixiang Liu,Ying WeiPubMed 35049870
- Frontiers in oncology 11:7472822021β-Lapachone Selectively Kills Hepatocellular Carcinoma Cells by Targeting NQO1 to Induce Extensive DNA Damage and PARP1 Hyperactivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenxiu Zhao,Lingxiang Jiang,Ting Fang,Fei Fang,Yingchun Liu,Ye Zhao,Yuting You,Hao Zhou,Xiaolin Su,Jiangwei Wang,Sheng Liu,Yaomin Chen,Jun Wan,Xiumei HuangPubMed 34676172
- PLoS genetics 17:e10097322021High-throughput screening and genome-wide analyses of 44 anticancer drugs in the 1000 Genomes cell lines reveals an association of the NQO1 gene with the response of multiple anticancer drugs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFarida S Akhtari,Adrian J Green,George W Small,Tammy M Havener,John S House,Kyle R Roell,David M Reif,Howard L McLeod,Timothy Wiltshire,Alison A Motsinger-ReifPubMed 34437536
- Antioxidants (Basel, Switzerland) 10:2021Discovering the Protective Effects of Resveratrol on Aflatoxin B1-Induced Toxicity: A Whole Transcriptomic Study in a Bovine Hepatocyte Cell Line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarianna Pauletto,Mery Giantin,Roberta Tolosi,Irene Bassan,Andrea Barbarossa,Anna Zaghini,Mauro DacastoPubMed 34439473
- Antioxidants (Basel, Switzerland) 9:2020Curcumin Mitigates AFB1-Induced Hepatic Toxicity by Triggering Cattle Antioxidant and Anti-inflammatory Pathways: A Whole Transcriptomic In Vitro Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarianna Pauletto et. al.PubMed 33137966
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell culture extracts, Suspension cells, Tissue Extracts, Tissue Homogenate, Adherent cells, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Mouse, Rat, Cow, Human
- Assay time
- 30m
Target data
Function
Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species (By similarity) (PubMed:8999809, PubMed:9271353). Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion (PubMed:15102952, PubMed:8999809, PubMed:9271353). Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential (PubMed:8999809). Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome (PubMed:15687255, PubMed:28291250).
Alternative names
DIA4, NMOR1, NQO1, NAD(P)H dehydrogenase [quinone] 1, Azoreductase, DT-diaphorase, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, DTD, QR1
What's included?
Recommended products
NQO1 Activity Assay kit (ab184867) is designed for the sensitive and accurate measurement of NQO1 (NAD(P)H dehydrogenase [quinone] 1) activity.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell culture extracts, Suspension cells, Tissue Extracts, Tissue Homogenate, Adherent cells, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Mouse, Rat, Cow, Human
- Assay time
- 30m
- Assay Platform
- Microplate reader
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 2.183 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 9 | mean - | SD - | C.V. 6.035 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
NQO1 Activity Assay kit (ab184867) is designed for the sensitive and accurate measurement of NQO1 (NAD(P)H dehydrogenase [quinone] 1) activity.
The enzyme activity is determined by following the reduction of Menadione with cofactor NADH and the simultaneous reduction of WST1 which leads to increased absorbance at 440 nm. Dicoumarol is used as an inhibitor of NQO1 as part of the assay.
NQO1 assay protocol summary:
- add samples to wells
- add reaction buffer + inhibitor and reaction buffer to separate sample wells
- analyze with a microplate reader for 5 min
NQO1 serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways, NQO1 protects against quinone-induced damage by competing with potentially toxic one-electron pathways. It also functions in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NQO1 also known as NAD(P)H:quinone oxidoreductase 1 is a cytosolic enzyme involved in two-electron reduction processes. This protein plays a role in detoxification transforming quinones into less reactive and harmful hydroquinones. NQO1 has a molecular weight of approximately 31 kDa and is present in various tissues with high expression in the liver and lungs. Sometimes called DT-diaphorase it contributes to antioxidant protection within cells and assists in the stabilization of other proteins such as p53.
- Biological function summary
NQO1 serves a protective function against oxidative stress by reducing quinones and preventing redox cycling that generates reactive oxygen species. Part of a critical network its function integrates with the cellular defense mechanism assisting in maintaining cellular homeostasis. NQO1 helps metabolize xenobiotics and is associated with phase II detoxification working alongside enzymes like glutathione S-transferases but is not part of a complex.
- Pathways
NQO1 is an important component of the antioxidant defense pathway participating in the direct enzymatic reduction of quinones protecting cells from oxidative damage. It also interfaces with the KEAP1-NRF2 pathway where the NQO1 gene is regulated by the NRF2 transcription factor that upregulates its expression in response to oxidative stress. NQO1 interlinks with proteins such as p53 through pathways related to apoptotic regulation and cellular stress responses.
- Associated diseases and disorders
Mutations or altered expression of NQO1 have correlations with cancer development and progression notably in liver and lung cancers. This protein's stability influences cancer cell survival particularly under oxidative stress or chemotherapeutic treatments. NQO1 also exhibits links to Alzheimer's disease where its potential role in neuroprotection against oxidative damage draws investigation relating it to proteins like tau and amyloid-beta.
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6 product images
Functional Studies - NQO1 Activity Assay Kit (ab184867)
Raw data from various concentrations of HepG2 cell extracts.
An example is shown below where the rate/slope is calculated between these time points.
Functional Studies - NQO1 Activity Assay Kit (ab184867)
NQO1 Activity in HepG2 extract.
This raw data is expressed as rate (mOD/min) per microgram of cell extract added per well as shown above. The molar extinction coefficient factor for the WST1 dye is 25.9/mM/well.
Functional Studies - NQO1 Activity Assay Kit (ab184867)
Raw data of NQO1 activity from 50 μg/mL of a series of normal samples.
Figure 3a
Functional Studies - NQO1 Activity Assay Kit (ab184867)
Comparison of NQO1 activity in different samples at 50 ug/mL.
Figure 3b
Functional Studies - NQO1 Activity Assay Kit (ab184867)
Dicoumarol sensitive NQO1 activity/total activity in a series of normal cell lysates at 50 ug/mL.
Figure 3c
Functional Studies - NQO1 Activity Assay Kit (ab184867)
NQO1 activity assay components requirement test.
Figure 4
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