Skip to main content

Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence | Cytopainter (ab139474) contains two proprietary dyes suitable for simultaneous live-cell staining of nucleoli and nuclei.

Be the first to review this product! Submit a review

Images

Fluorescence Microscopy - Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter (AB139474), expandable thumbnail

Publications

Key facts

Sample types
Suspension cells, Adherent cells
Assay type
Cell-based
Reactive species
Mammals
Assay time
30m

What's included?

500 Test
Components
10X Assay Buffer
1 x 15 mL
Actinomycin D Control
1 x 125 µg
Green Detection Reagent
1 x 50 µL
Red Detection Reagent
1 x 50 µL

Recommended products

Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence | Cytopainter (ab139474) contains two proprietary dyes suitable for simultaneous live-cell staining of nucleoli and nuclei.

Key facts

Sample types
Suspension cells, Adherent cells
Assay type
Cell-based
Reactive species
Mammals
Assay time
30m
Assay Platform
Fluorescence microscope

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
-80°C

Notes

Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence | Cytopainter (ab139474) contains two proprietary dyes suitable for simultaneous live-cell staining of nucleoli and nuclei. The dyes allow examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.This kit is specifically designed for visualizing nucleoli and nuclei in living cells. A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.Review other dyes and kits for , or the

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Fluorescence Microscopy - Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter (ab139474), expandable thumbnail

    Fluorescence Microscopy - Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter (ab139474)

    Absorption and fluorescence emission spectra for ab139474 Green (A) and Red (B) dyes. All spectra were determined in 1X Assay Buffer.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com