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AB139474

Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter

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(1 Publication)

Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence | Cytopainter (ab139474) contains two proprietary dyes suitable for simultaneous live-cell staining of nucleoli and nuclei.
1 Images
Fluorescence Microscopy - Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter (AB139474)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter (AB139474)

Absorption and fluorescence emission spectra for ab139474 Green (A) and Red (B) dyes. All spectra were determined in 1X Assay Buffer.

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay time

30m

Assay Platform

Fluorescence microscope

Product details

Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence | Cytopainter (ab139474) contains two proprietary dyes suitable for simultaneous live-cell staining of nucleoli and nuclei. The dyes allow examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.This kit is specifically designed for visualizing nucleoli and nuclei in living cells. A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.Review other dyes and kits for , or the

What's included?

{ "values": { "500Test": { "sellingSize": "500 Test", "publicAssetCode":"ab139474-500Test", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"Red Detection Reagent", "number":"AB139474-CMP02", "productcode":"" }, { "size":"1 x 125 µg", "name":"Actinomycin D Control", "number":"AB139474-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Assay Buffer", "number":"AB139474-CMP03", "productcode":"" }, { "size":"1 x 50 µL", "name":"Green Detection Reagent", "number":"AB139474-CMP01", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
-80°C

Product protocols

Target data

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 66:889-902 PubMed30138043

2018

Three-Dimensional Cellular Raman Analysis: Evidence of Highly Ordered Lipids Within Cell Nuclei.

Applications

Unspecified application

Species

Unspecified reactive species

Bhagavathi Ramamurthy,Stanley Cohen,Mark Canales,Frederick D Coffman
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