JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB139475

Nucleolar Staining Kit - Green Fluorescence - Cytopainter

0

(1 Review)

|

(3 Publications)

Nucleolar Staining Kit - Green Fluorescence | Cytopainter (ab139475) contains a proprietary dye suitable for live-cell staining of nucleoli.
1 Images
Fluorescence Microscopy - Nucleolar Staining Kit - Green Fluorescence - Cytopainter (AB139475)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Nucleolar Staining Kit - Green Fluorescence - Cytopainter (AB139475)

Absorption and fluorescence emission spectra for Green Detection Reagent dye. Spectrum was determined in 1X Assay Buffer.

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay time

30m

Assay Platform

Fluorescence microscope

Product details

Nucleolar Staining Kit - Green Fluorescence | Cytopainter (ab139475) contains a proprietary dye suitable for live-cell staining of nucleoli. The dye allows examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.ab139475 is specifically designed for visualizing nucleoli in living cells. The dye in the kit is resistant to photobleaching, facilitating its use in imaging applications. This nucleolar dye may be used in conjunction with common live-cell nuclear counterstains such as Hoechst 33342, Draq5™ or Nuclear Red dye.A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition of transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.Review other dyes and kits for , or the

What's included?

{ "values": { "500Test": { "sellingSize": "500 Test", "publicAssetCode":"ab139475-500Test", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"10X Assay Buffer", "number":"AB139475-CMP02", "productcode":"" }, { "size":"1 x 125 µg", "name":"Actinomycin D Control", "number":"AB139475-CMP03", "productcode":"" }, { "size":"1 x 50 µL", "name":"Green Detection Reagent", "number":"AB139475-CMP01", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
-80°C

Product protocols

Target data

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed38214505

2024

Probe-free optical chromatin deformation and measurement of differential mechanical properties in the nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Seelbinder,Susan Wagner,Manavi Jain,Elena Erben,Sergei Klykov,Iliya Dimitrov Stoev,Venkat Raghavan Krishnaswamy,Moritz Kreysing

Nanoscale advances 3:249-262 PubMed36131874

2020

Nano-scale imaging of dual stable isotope labeled oxaliplatin in human colon cancer cells reveals the nucleolus as a putative node for therapeutic effect.

Applications

Unspecified application

Species

Unspecified reactive species

Anton A Legin,Arno Schintlmeister,Nadine S Sommerfeld,Margret Eckhard,Sarah Theiner,Siegfried Reipert,Daniel Strohhofer,Michael A Jakupec,Mathea S Galanski,Michael Wagner,Bernhard K Keppler

Nature structural & molecular biology 25:176-184 PubMed29343869

2018

Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING).

Applications

Unspecified application

Species

Unspecified reactive species

Philipp G Maass,A Rasim Barutcu,David M Shechner,Catherine L Weiner,Marta Melé,John L Rinn
View all publications
websiteProtocolBooklet
en

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com