Oil Red O (Lipid Stain) kit ab150678 used to stain and visualise fat cells and neutral fat in tissue sections.
- Use to stain lipids and adipocytes in frozen tissue sections, fresh smears, or touch preps
- Includes ready-to-use Oil Red O and Hematoxylin solutions
- Cited in over 100 publications
Images
Publications
Filter by
- Nature 628:154-1612024APOE4/4 is linked to damaging lipid droplets in Alzheimer's disease microglia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael S Haney,Róbert Pálovics,Christy Nicole Munson,Chris Long,Patrik K Johansson,Oscar Yip,Wentao Dong,Eshaan Rawat,Elizabeth West,Johannes C M Schlachetzki,Andy Tsai,Ian Hunter Guldner,Bhawika S Lamichhane,Amanda Smith,Nicholas Schaum,Kruti Calcuttawala,Andrew Shin,Yung-Hua Wang,Chengzhong Wang,Nicole Koutsodendris,Geidy E Serrano,Thomas G Beach,Eric M Reiman,Christopher K Glass,Monther Abu-Remaileh,Annika Enejder,Yadong Huang,Tony Wyss-CorayPubMed 38480892
- Cell death discovery 9:4562023Pharmacological inhibition of MALT1 (mucosa-associated lymphoid tissue lymphoma translocation protein 1) induces ferroptosis in vascular smooth muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBinjie Yan,Darrell Belke,Yu Gui,Yong-Xiang Chen,Zhi-Sheng Jiang,Xi-Long ZhengPubMed 38097554
- Frontiers in physiology 14:12920332023Lifetime evaluation of left ventricular structure and function in male ApoE null mice after gamma and space-type radiation exposure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnieszka Brojakowska,Cedric J Jackson,Malik Bisserier,Mary K Khlgatian,Vineeta Jagana,Abrisham Eskandari,Cynthia Grano,Steve R Blattnig,Shihong Zhang,Kenneth M Fish,Vadim Chepurko,Elena Chepurko,Virginia Gillespie,Ying Dai,Amit Kumar Rai,Venkata Naga Srikanth Garikipati,Lahouaria Hadri,Raj Kishore,David A GoukassianPubMed 38054039
- eLife 12:2023Human skeletal muscle organoids model fetal myogenesis and sustain uncommitted PAX7 myogenic progenitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLampros Mavrommatis,Hyun-Woo Jeong,Urs Kindler,Gemma Gomez-Giro,Marie-Cecile Kienitz,Martin Stehling,Olympia E Psathaki,Dagmar Zeuschner,M Gabriele Bixel,Dong Han,Gabriela Morosan-Puopolo,Daniela Gerovska,Ji Hun Yang,Jeong Beom Kim,Marcos J Arauzo-Bravo,Jens C Schwamborn,Stephan A Hahn,Ralf H Adams,Hans R Schöler,Matthias Vorgerd,Beate Brand-Saberi,Holm ZaehresPubMed 37963071
- Journal of orthopaedic surgery and research 18:6222023Psoralen synergizes with exosome-loaded SPC25 to alleviate senescence of nucleus pulposus cells in intervertebral disc degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Yang,Zhaoyong Li,Chao Zhang,Shuofu Li,Long Chen,Shaofeng Yang,Yantao GuoPubMed 37872583
- Antioxidants (Basel, Switzerland) 12:2023NRF2 Deficiency Attenuates Diabetic Kidney Disease in Db/Db Mice via Down-Regulation of Angiotensinogen, SGLT2, CD36, and FABP4 Expression and Lipid Accumulation in Renal Proximal Tubular Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Su,Shui-Ling Zhao,Wen-Xia Yang,Chao-Sheng Lo,Isabelle Chenier,Min-Chun Liao,Yu-Chao Pang,Jun-Zheng Peng,Kana N Miyata,Jean-Francois Cailhier,Jean Ethier,Jean-Baptiste Lattouf,Janos G Filep,Julie R Ingelfinger,Shao-Ling Zhang,John S D ChanPubMed 37760019
- Viruses 15:2023Adipose Tissues from Human and Bat-Derived Cell Lines Support Ebola Virus Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLauren Garnett,Kaylie N Tran,Zachary Schiffman,Kristina A Muise,Quinn E Fletcher,Yvonne A Dzal,Anders Leung,Alix Albietz,Bryce M Warner,Bryan D Griffin,Darwyn Kobasa,Craig K R Willis,James E StrongPubMed 37766234
- Nature communications 14:51062023Impaired Plakophilin-2 in obesity breaks cell cycle dynamics to breed adipocyte senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAina Lluch,Jessica Latorre,Angela Serena-Maione,Isabel Espadas,Estefanía Caballano-Infantes,José M Moreno-Navarrete,Núria Oliveras-Cañellas,Wifredo Ricart,María M Malagón,Alejandro Martin-Montalvo,Walter Birchmeier,Witold Szymanski,Johannes Graumann,María Gómez-Serrano,Elena Sommariva,José M Fernández-Real,Francisco J OrtegaPubMed 37607954
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e231142023Convergent alteration of the mesenchymal stem cell heterogeneity in adipose tissue during aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiwei Liu,Yu Zhao,Qian Wang,Li Yan,Xin Fu,Ran XiaoPubMed 37498236
- BMC complementary medicine and therapies 23:2092023Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Chen,Rong Rui,Li Wang,Hao Wang,Bingbing Zhu,Aili CaoPubMed 37365530
Associated Products
Select an associated product type
2 products for Alternative Product
What's included?
Recommended products
Oil Red O (Lipid Stain) kit ab150678 used to stain and visualise fat cells and neutral fat in tissue sections.
- Use to stain lipids and adipocytes in frozen tissue sections, fresh smears, or touch preps
- Includes ready-to-use Oil Red O and Hematoxylin solutions
- Cited in over 100 publications
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- Ambient
Notes
This Oil Red O (Lipid Stain) uses Oil Red O that is a fat-soluble dye.Fat staining occurs by absorption of oil red O into lipoid substances. This is a physical method of staining that relies on greater solubility of oil red O in the lipoid substance than in the dye solvent. This kit may be used ONLY on frozen tissue sections, fresh smears, or touch preps as xylenes and alcohols will dissolve fat deposits. It cannot be used with formaldehyde-fixed paraffin embedded sections as the alcohols used remove most lipids.
Oil Red O staining protocol summary:
- Prepare fresh or frozen tissue sections
- Incubate slide in propylene glycol for 2 min
- Incubate slide in oil red o solution for 6 min
- Differentiate section in 85% propylene glycol for 1 min
- Rinse slide twice in water
- Incubate in hematoxylin for 1-2 min
- Rinse slide three times in water
- Coverslip with an aqueous mounting medium
How other researchers are using
Oil Red O Stain Kit (Lipid Stain) has been used in a variety of sample type including:
- Human brain tissue 1
- Mouse arterial tissues 2
- Mouse Aortas tissues 3
References:
1 - Haney M et al. 2024; 2 - Yan B et al. 2023; 3 - Brojakowska A et al. 2023.
Related and recommended products
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678)
ab150678 Oil Red O Stain Kit (Lipid Stain) staining frozen human human adipose.
Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678)
ab150678 (Oil Red O stain) staining lipid deposits (red) in frozen normal human skin. The dermis is unstained, whereas the hypodermis is stained, demonstrating dye specificity. Hematoxylin (blue) is used as a counterstain.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678)
Oil Red O staining of 3T3-L1 and adipocyte-like cells
During adipocyte differentiation, cells accumulate lipid. Oil Red O staining kit (ab150678) was used to visualize lipid in 3T3-L1 cells (left) and adipocyte-like cells (right). As expected, lipid accumulates in 3T3-L1 cells after differentiation into adipocyte-like cells.
Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678)
Staining of Frozen Breast Tissue using ab150678 - Oil Red O Stain Kit.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com