Oil Red O (Lipid Stain) kit ab150678 used to stain and visualise fat cells and neutral fat in tissue sections.
- Use to stain lipids and adipocytes in frozen tissue sections, fresh smears, or touch preps
- Includes ready-to-use Oil Red O and Hematoxylin solutions
- Cited in over 100 publications
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Oil Red O (Lipid Stain) kit ab150678 used to stain and visualise fat cells and neutral fat in tissue sections.
- Use to stain lipids and adipocytes in frozen tissue sections, fresh smears, or touch preps
- Includes ready-to-use Oil Red O and Hematoxylin solutions
- Cited in over 100 publications
This Oil Red O (Lipid Stain) uses Oil Red O that is a fat-soluble dye.Fat staining occurs by absorption of oil red O into lipoid substances. This is a physical method of staining that relies on greater solubility of oil red O in the lipoid substance than in the dye solvent. This kit may be used ONLY on frozen tissue sections, fresh smears, or touch preps as xylenes and alcohols will dissolve fat deposits. It cannot be used with formaldehyde-fixed paraffin embedded sections as the alcohols used remove most lipids.
Oil Red O staining protocol summary:
- Prepare fresh or frozen tissue sections
- Incubate slide in propylene glycol for 2 min
- Incubate slide in oil red o solution for 6 min
- Differentiate section in 85% propylene glycol for 1 min
- Rinse slide twice in water
- Incubate in hematoxylin for 1-2 min
- Rinse slide three times in water
- Coverslip with an aqueous mounting medium
How other researchers are using
Oil Red O Stain Kit (Lipid Stain) has been used in a variety of sample type including:
- Human brain tissue 1
- Mouse arterial tissues 2
- Mouse Aortas tissues 3
References:
1 - Haney M et al. 2024; 2 - Yan B et al. 2023; 3 - Brojakowska A et al. 2023.
Related and recommended products
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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ab150678 Oil Red O Stain Kit (Lipid Stain) staining frozen human human adipose.
ab150678 (Oil Red O stain) staining lipid deposits (red) in frozen normal human skin. The dermis is unstained, whereas the hypodermis is stained, demonstrating dye specificity. Hematoxylin (blue) is used as a counterstain.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
Oil Red O staining of 3T3-L1 and adipocyte-like cells
During adipocyte differentiation, cells accumulate lipid. Oil Red O staining kit (ab150678) was used to visualize lipid in 3T3-L1 cells (left) and adipocyte-like cells (right). As expected, lipid accumulates in 3T3-L1 cells after differentiation into adipocyte-like cells.
Staining of Frozen Breast Tissue using ab150678 - Oil Red O Stain Kit.
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