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Oil Red O (Lipid Stain) kit ab150678 used to stain and visualise fat cells and neutral fat in tissue sections.

- Use to stain lipids and adipocytes in frozen tissue sections, fresh smears, or touch preps
- Includes ready-to-use Oil Red O and Hematoxylin solutions
- Cited in over 100 publications


Images

Histology - Oil Red O Stain Kit (Lipid Stain) (AB150678), expandable thumbnail
  • Histology - Oil Red O Stain Kit (Lipid Stain) (AB150678), expandable thumbnail
  • Histology - Oil Red O Stain Kit (Lipid Stain) (AB150678), expandable thumbnail
  • Histology - Oil Red O Stain Kit (Lipid Stain) (AB150678), expandable thumbnail

Publications

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What's included?

100 Test
Components
Hematoxylin (Modified Mayer's Solution)
1 x 125 mL
Oil Red O Solution
1 x 125 mL
Propylene Glycol
1 x 500 mL

Recommended products

Oil Red O (Lipid Stain) kit ab150678 used to stain and visualise fat cells and neutral fat in tissue sections.

- Use to stain lipids and adipocytes in frozen tissue sections, fresh smears, or touch preps
- Includes ready-to-use Oil Red O and Hematoxylin solutions
- Cited in over 100 publications

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient

Notes

How the assay works

This Oil Red O (Lipid Stain) uses Oil Red O that is a fat-soluble dye.Fat staining occurs by absorption of oil red O into lipoid substances. This is a physical method of staining that relies on greater solubility of oil red O in the lipoid substance than in the dye solvent. This kit may be used ONLY on frozen tissue sections, fresh smears, or touch preps as xylenes and alcohols will dissolve fat deposits. It cannot be used with formaldehyde-fixed paraffin embedded sections as the alcohols used remove most lipids.

Oil Red O staining protocol summary:

- Prepare fresh or frozen tissue sections
- Incubate slide in propylene glycol for 2 min
- Incubate slide in oil red o solution for 6 min
- Differentiate section in 85% propylene glycol for 1 min
- Rinse slide twice in water
- Incubate in hematoxylin for 1-2 min
- Rinse slide three times in water
- Coverslip with an aqueous mounting medium

How other researchers are using

Oil Red O Stain Kit (Lipid Stain) has been used in a variety of sample type including:
- Human brain tissue 1
- Mouse arterial tissues 2
- Mouse Aortas tissues 3

References:
1 - Haney M et al. 2024; 2 - Yan B et al. 2023; 3 - Brojakowska A et al. 2023.

Related and recommended products

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678), expandable thumbnail

    Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678)

    ab150678 Oil Red O Stain Kit (Lipid Stain) staining frozen human human adipose.

  • Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678), expandable thumbnail

    Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678)

    ab150678 (Oil Red O stain) staining lipid deposits (red) in frozen normal human skin. The dermis is unstained, whereas the hypodermis is stained, demonstrating dye specificity. Hematoxylin (blue) is used as a counterstain.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678), expandable thumbnail

    Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678)

    Oil Red O staining of 3T3-L1 and adipocyte-like cells

    During adipocyte differentiation, cells accumulate lipid. Oil Red O staining kit (ab150678) was used to visualize lipid in 3T3-L1 cells (left) and adipocyte-like cells (right). As expected, lipid accumulates in 3T3-L1 cells after differentiation into adipocyte-like cells.

  • Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678), expandable thumbnail

    Histology - Oil Red O Stain Kit (Lipid Stain) (ab150678)

    Staining of Frozen Breast Tissue using ab150678 - Oil Red O Stain Kit.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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