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AB133414

Optiblot SDS-PAGE Sample Preparation Kit

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The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.
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Western blot - Optiblot SDS-PAGE Sample Preparation Kit (AB133414)
  • WB

Supplier Data

Western blot - Optiblot SDS-PAGE Sample Preparation Kit (AB133414)

Resin concentration is compatible with Western blot detection. Western blots were created with samples of A431 cell extract (panels a and b, lane 2), diluted cell extract (lane 3) and the diluted extract concentrated using the Optiblot SDS-PAGE Sample Preparation Kit protocol (lane 4). The blots were detected using the Optiblot Fluorescent Western Blot Kit (ab133410). No bands were detectable in the diluted cell extract when stained for either GAPDH (panel a, lane 3) or SRC (panel b, lane 3). However, after resin concentration, bands are easily visualized for both proteins (lanes 4). Band intensities are indicated.

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Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Purification": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.

The fast, efficient protocol generates samples ready to load on a gel in less than ten minutes, much more quickly than can be achieved with alternate methods such as dialysis, or acetone or TCA precipitation. Additionally, the Optiblot SDS-PAGE Sample Preparation protocol is easily scaled up, allowing multiple samples to be prepared in parallel.

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What's included?

{ "values": { "25Test": { "sellingSize": "25 Test", "publicAssetCode":"ab133414-25Test", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Extraction loading buffer", "number":"AB133414-CMP01" }, { "size":"25 x 1 Unit", "name":"Protein filters", "number":"AB133414-CMP02" }, { "size":"2 x 500 mL", "name":"Resin", "number":"AB133414-CMP03" } ] }, "10Test": { "sellingSize": "10 Test", "publicAssetCode":"ab133414-10Test", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Extraction loading buffer", "number":"AB133414-CMP01", "productcode":"" }, { "size":"10 x 1 Unit", "name":"Protein filters", "number":"AB133414-CMP02", "productcode":"" }, { "size":"1 x 500 mL", "name":"Resin", "number":"AB133414-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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