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Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab138898) is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP).

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Flow Cytometry - Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (AB138898), expandable thumbnail

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Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Direct

What's included?

100 Test
Components
Assay Buffer
1 x 100 mL
MitoOrange Dye
1 x 200 µL

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Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab138898) is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP).

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Direct
Assay Platform
Fluorescence microscope

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab138898) is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP). The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.

ab138898 provides all the essential components with an optimized assay method. This fluorimetric assay uses our proprietary cationic MitoOrange Dye for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. In normal cells, the red fluorescence intensity is increased when MitoOrange Dye is accumulated in the mitochondria. However, in apoptotic cells, the fluorescence intensity of MitoOrange Dye is decreased following the collapse of MMP. Cells stained with MitoOrange Dye can be visualized with a flow cytometer at 488 nm excitation with red emission (FL2 channel). The kit can be used together with other reagents for multi-parametric study of cell vitality and apoptosis. The kit is optimized for screening apoptosis activators and inhibitors with a flow cytometer.

**Related assays**Review the to learn about kits to perform a , and . Review the to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

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1 product image

  • Flow Cytometry - Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab138898), expandable thumbnail

    Flow Cytometry - Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab138898)

    Flow Cytometric analysis of Jurkat cells. Flow Cytometric analysis demonstrating the decrease in fluorescence intensity of MitoOrange Dye with the addition of FCCP in Jurkat cells. Jurkat cells were loaded with MitoOrange Dye
    alone (Blue) or in the presence of 30 µM FCCP (Red) for 15 minutes. The fluorescence intensity of MitoOrange Dye was measured with a flow cytometer using the FL2 channel.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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