Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry)

Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab138898) is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP). The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.

ab138898 provides all the essential components with an optimized assay method. This fluorimetric assay uses our proprietary cationic MitoOrange Dye for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. In normal cells, the red fluorescence intensity is increased when MitoOrange Dye is accumulated in the mitochondria. However, in apoptotic cells, the fluorescence intensity of MitoOrange Dye is decreased following the collapse of MMP. Cells stained with MitoOrange Dye can be visualized with a flow cytometer at 488 nm excitation with red emission (FL2 channel). The kit can be used together with other reagents for multi-parametric study of cell vitality and apoptosis. The kit is optimized for screening apoptosis activators and inhibitors with a flow cytometer.

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