Orange Mitochondrial Membrane Potential Assay Kit (Microplate)

Orange Mitochondrial Membrane Potential Assay Kit (Microplate) is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP). The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.

ab138899 uses our proprietary cationic MitoOrange Dye for the detection of the mitochondrial membrane potential change in cells. In normal cells, the orange fluorescence intensity is increased when MitoOrange Dye is accumulated in the mitochondria. However, in apoptotic cells, the fluorescence intensity of MitoOrange Dye is decreased following the collapse of MMP. Cells stained with MitoOrange Dye can be fluorometrically monitored at Ex/Em = 540/590 nm. ab138899 provides all the essential components with an optimized assay method. The kit can be used for screening activators and inhibitors of apoptosis. And the assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format without a wash step.