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Orange Mitochondrial Membrane Potential Assay Kit (Microplate) is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP).

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Images

Functional Studies - Orange Mitochondrial Membrane Potential Assay Kit (Microplate) (AB138899), expandable thumbnail

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Direct

What's included?

5 x 96 Tests
Components
Assay Buffer A
1 x 50 mL
Assay Buffer B
1 x 25 mL
MitoOrange Dye
1 x 250 µL

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Orange Mitochondrial Membrane Potential Assay Kit (Microplate) is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP).

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Direct
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Orange Mitochondrial Membrane Potential Assay Kit (Microplate) is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP). The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.

ab138899 uses our proprietary cationic MitoOrange Dye for the detection of the mitochondrial membrane potential change in cells. In normal cells, the orange fluorescence intensity is increased when MitoOrange Dye is accumulated in the mitochondria. However, in apoptotic cells, the fluorescence intensity of MitoOrange Dye is decreased following the collapse of MMP. Cells stained with MitoOrange Dye can be fluorometrically monitored at Ex/Em = 540/590 nm. ab138899 provides all the essential components with an optimized assay method. The kit can be used for screening activators and inhibitors of apoptosis. And the assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format without a wash step.

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1 product image

  • Functional Studies - Orange Mitochondrial Membrane Potential Assay Kit (Microplate) (ab138899), expandable thumbnail

    Functional Studies - Orange Mitochondrial Membrane Potential Assay Kit (Microplate) (ab138899)

    Demonstation of decrease in MitoOrange Dye fluorescence with the addition of FCCP in HeLa cells. The decrease in MitoOrange Dye fluorescence with the addition of FCCP in HeLa cells. HeLa cells were dye loaded with MitoOrange Dye alone or in the presence of 20 µM FCCP for 15 minutes. The fluorescence intensity of MitoOrange Dye was measured 30 minutes after adding Assay Buffer B (Component C) with a microplate reader at Ex/Em = 540/590 nm (cut off 570 nm, bottom read).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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