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AB245881

Orcein Stain Kit (Hepatitis B and Elastic Fibers)

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(4 Publications)

Orcein Stain Kit (Hepatitis B and Elastic Fibers) ab245881 may be used in histology procedures for the visualization of Hepatitis B surface Antigen (HBsAg), elastic fibers, and copper associated proteins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Orcein Stain Kit (Hepatitis B and Elastic Fibers) (AB245881)
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Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Orcein Stain Kit (Hepatitis B and Elastic Fibers) (AB245881)

ab245881 Orcein Stain Kit (Hepatitis B and Elastic Fibers) staining formalin-fixed-paraffin embedded tissue section.

Key facts

Sample types

Tissue sections

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Product details

Orcein Stain Kit (Hepatitis B and Elastic Fibers) ab245881 may be used in histology procedures for the visualization of Hepatitis B surface Antigen (HBsAg), elastic fibers, and copper associated proteins. HBsAg appears as irregular shaped aggregates in the cytoplasmic region of the cells. This reagent may be used on formalin-fixed, paraffin-embedded sections.HBsAg: Dark Red/Brown Elastic Fibers: Dark Red/Brown Copper Assoc. Proteins: Dark Red/Brown Background: Light Reddish/Purple**Control Tissue:** Known hepatitis positive liver. Lung for elastic fiber. Any well fixed tissue cut 3-5 microns.**Other products for staining tissue sections**Find more kits and reagents in the , or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the .
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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab245881-1Kit", "assetComponentDetails": [ { "size":"1 x 125 mL", "name":"Differentiating Solution", "number":"AB245881-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Potassium Permanganate Sol. (5%)", "number":"AB245881-CMP04", "productcode":"" }, { "size":"1 x 125 mL", "name":"Oxalic Acid Dihydrate", "number":"AB245881-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sulfuric Acid Solution (3%)", "number":"AB245881-CMP05", "productcode":"" }, { "size":"1 x 125 mL", "name":"Orcein Solution", "number":"AB245881-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepatitis B virus (HBV) is a small DNA virus with a mass of about 3.2 kilobases known to cause liver infection. It primarily targets hepatocytes the main cells of the liver resulting in inflammation and damage. Orcein staining a technique known for highlighting elastic fiber structures in tissue samples occasionally detects HBV as orcein dye binds to the virus-infected cells highlighting elastic fibers in histology studies. Orcein-derived stains are also known to be useful in the histological analysis of liver biopsies when diagnosing HBV infection. This virus is also referred to as serum hepatitis given it is transmitted through exposure to infectious body fluids such as blood.
Biological function summary

The hepatitis B virus affects liver cell function by integrating into the host genome influencing liver metabolism and immune responses. It forms a complex with the hepatitis B surface antigen (HBsAg) that facilitates viral assembly and secretion. The orcein stain histology method helps reveal changes in liver architecture as a result of ongoing HBV replication illustrating the impact on elastic fibers within tissues. Elastic fiber staining such as with orcein further assists in understanding the tissue-level changes in chronic hepatitis patients.

Pathways

Hepatitis B virus influences several cellular processes including the PI3K/Akt pathway. This pathway affects cell survival proliferation and metabolism often associated with hepatitis B surface antigen. HBV can modulate the NF-kB pathway which is important for immune responses and inflammation regulation. Through these pathways HBV affects interactions with other proteins such as Akt and NF-kB important for maintaining cell homeostasis and defense mechanisms against viral infections.

Hepatitis B virus is a leading cause of chronic hepatitis and liver cancer (hepatocellular carcinoma). Chronic infection with HBV elevates inflammation and fibrosis often detected through histological methods like orcein staining. This connection emphasizes the relationship between HBV and altered extracellular matrix components including elastic fibers. Additionally proteins such as p53 involved in cell cycle regulation and apoptosis may interact with HBV during carcinogenic processes highlighting its role in liver disease progression.
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Product protocols

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Target data

See full target information Orcein
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

European heart journal open 5:oeaf021 PubMed40352252

2025

Fluoroquinolones and the risk of aortic aneurysm or aortic dissection: evidence from a nationwide nested case-control study paralleled with matched experimental models.

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Unspecified application

Species

Unspecified reactive species

Callan D Wesley,Jarl Emanuel Strange,Anders Holt,Gunnar H Gislason,Cédric H G Neutel,Dustin N Krüger,Celine Civati,Mart Theunis,Tania Naessens,Lynn Roth,Guido R Y De Meyer,Wim Martinet,Peter Vibe Rasmussen,Pieter-Jan Guns

Scientific reports 14:18337 PubMed39112507

2024

Unravelling the impact of active and passive contributors to arterial stiffness in male mice and their role in vascular aging.

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Unspecified application

Species

Unspecified reactive species

Callan D Wesley,Cedric H G Neutel,Guido R Y De Meyer,Wim Martinet,Pieter-Jan Guns

Nutrients 13: PubMed34836432

2021

Blackcurrant Improves Diabetic Cardiovascular Dysfunction by Reducing Inflammatory Cytokines in Type 2 Diabetes Mellitus Mice.

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Unspecified application

Species

Unspecified reactive species

Hye-Yoom Kim,Jung-Joo Yoon,Hyeon-Kyoung Lee,Ai-Lin Tai,Yun-Jung Lee,Dae-Sung Kim,Dae-Gill Kang,Ho-Sub Lee

Nutrients 13: PubMed34684415

2021

YG-1 Extract Improves Acute Pulmonary Inflammation by Inducing Bronchodilation and Inhibiting Inflammatory Cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Yoom Kim,Jung-Joo Yoon,Dae-Sung Kim,Dae-Gill Kang,Ho-Sub Lee
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