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The Overall Exosome Capture and Quantification Assay Kit ab287851 (Previously known as ExoQuant Overall Exosome Capture and Quantification AssayKit (Cell Media, Colorimetric) Kit), is a double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes.

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Detection method

Colorimetric

Sample types

Cell culture supernatant

Assay type

Sandwich

Reactive species

Human

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Images

Target data

What's included?

96 Test
Components
1X Sample buffer
2 x 10 mL
25X Washing buffer
1 x 25 mL
Exosome Standards
2 x 100 µg
HRP-Conjugate
1 x 5 µL
Immunoplate (Transparent)
1 x 1 Unit
Primary Antibody
1 x 20 µL
Stop Solution
1 x 10 mL
Substrate Chromogenic Solutions
1 x 10 mL

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The Overall Exosome Capture and Quantification Assay Kit ab287851 (Previously known as ExoQuant Overall Exosome Capture and Quantification AssayKit (Cell Media, Colorimetric) Kit), is a double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Assay type

Sandwich

Reactive species

Human

Assay Platform

Microplate (12 x 8 well strips)

Precision

Intra assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
< 10

Inter assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
< 13

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

The Overall Exosome Capture and Quantification Assay Kit ab287851 (Previously known as ExoQuant Overall Exosome Capture and Quantification AssayKit (Cell Media, Colorimetric) Kit), is a double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. The kit consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from cell culture supernatants. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively)

Applications: Overall exosome immunocapture and quantification from cell culture supernatant

Features & Benefits: Double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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