The Overall Exosome Capture and Quantification Assay Kit ab287851 (Previously known as ExoQuant Overall Exosome Capture and Quantification AssayKit (Cell Media, Colorimetric) Kit), is a double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant
- Assay type
- Sandwich
- Reactive species
- Human
Target data
Function
Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:14575715, PubMed:18541721, PubMed:8478605). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:7511626, PubMed:8478605).
Alternative names
CD9, MIC3, TSPAN29, GIG2, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29
What's included?
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The Overall Exosome Capture and Quantification Assay Kit ab287851 (Previously known as ExoQuant Overall Exosome Capture and Quantification AssayKit (Cell Media, Colorimetric) Kit), is a double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant
- Assay type
- Sandwich
- Reactive species
- Human
- Assay Platform
- Microplate (12 x 8 well strips)
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 13 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
The Overall Exosome Capture and Quantification Assay Kit ab287851 (Previously known as ExoQuant Overall Exosome Capture and Quantification AssayKit (Cell Media, Colorimetric) Kit), is a double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. The kit consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from cell culture supernatants. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively)
Applications: Overall exosome immunocapture and quantification from cell culture supernatant
Features & Benefits: Double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.
- Biological function summary
The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.
- Pathways
CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.
- Associated diseases and disorders
CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.
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4 product images
Western blot - Overall Exosome Capture and Quantification Assay Kit (ab287851)
Overall Exosome Capture and Quantification Assay Kit Western Blot.
CD9 marker detection by Western Blotting on lyophilized exosomes from human plasma and the recombinant CD9 protein.
Sandwich ELISA - Overall Exosome Capture and Quantification Assay Kit (ab287851)
Overall Exosome Capture and Quantification Assay Kit Standard Curve 1.
Example of standard curves obtained. Exosome standard preparation derived from COLO1 Cell Culture Supernatant
This standard curve is for demonstration only. For quantification purposes, a standard curve must be obtained with every assay.
Sandwich ELISA - Overall Exosome Capture and Quantification Assay Kit (ab287851)
Overall Exosome Capture and Quantification Assay Kit Standard Curve 2.
Example of standard curves obtained. Exosome standard preparation derived from Plasma of healthy donors.
This standard curve is for demonstration only. For quantification purposes, a standard curve must be obtained with every assay.
Sandwich ELISA - Overall Exosome Capture and Quantification Assay Kit (ab287851)
Overall Exosome Capture and Quantification Assay Kit.
CD9 titration of plasma healthy donor exosome standards.
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Product protocols
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