AB242302

Oxidized LDL Assay Kit (MDA-LDL, Human)

Name: Oxidized LDL Assay Kit (MDA-LDL, Human) (ab242302) | Abcam
Brand: Abcam Limited
SKU: AB242302
Price: 1020 USD
Availability: InStock

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(3 Publications)

Oxidized LDL Assay Kit (MDA-LDL, Human) ELISA Kit (ab242302) is developed for the detection and quantitation of oxidized phospholipids (OxPL) associated with human LDL in plasma, serum or other biological fluid samples.

2 Images

ELISA - Oxidized LDL Assay Kit (MDA-LDL, Human) (AB242302)

ELISA

Supplier Data

ELISA - Oxidized LDL Assay Kit (MDA-LDL, Human) (AB242302)

Human OxLDL Standard Curve

ELISA

Supplier Data

ELISA - Oxidized LDL Assay Kit (MDA-LDL, Human) (AB242302)

Quantitation of OxLDL in Serum and Plasma Samples.

Left : LDL Recovery After Precipitation Solution. Serum and plasma samples were treated with LDL Precipitation Solution according to the Sample Preparation protocol. LDL recovery was determined. Right : OxLDL Determination of Serum and Plasma Samples. Serum and plasma samples were treated with LDL Precipitation Solution according to the Sample Preparation Section. Precipitated LDL pellets were resuspended in 1.6 mL of PBS before further diluting 1 : 160 in Assay Diluent. Samples were tested according to the Assay Protocol.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Sensitivity

= 50 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

The kit contains an OxLDL Standard and has a detection sensitivity limit of <15 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242302-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Assay Diluent", "number":"AB242302-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Substrate Solution", "number":"AB242302-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-MDA Antibody Coated Plate", "number":"AB242302-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242302-CMP08", "productcode":"" }, { "size":"1 x 100 mL", "name":"10X Wash Buffer", "number":"AB242302-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"Blocking Reagent (100X)", "number":"AB242302-CMP05", "productcode":"" }, { "size":"1 x 25 µL", "name":"OxLDL Standard", "number":"AB242302-CMP07", "productcode":"" }, { "size":"1 x 20 µL", "name":"Streptavidin-Enzyme Conjugate", "number":"AB242302-CMP09", "productcode":"" }, { "size":"1 x 20 µL", "name":"Biotinylated Anti-Human ApoB-100 Antibody (1000X)", "number":"AB242302-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"LDL Precipitation Solution (2X)", "number":"AB242302-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oxidized phospholipids often referred to as oxidized LDL or ox-LDL play a role in lipid metabolism and atherosclerosis development. Ox-LDL results from the oxidative modification of low-density lipoprotein (LDL) molecules heavily impacting cardiovascular health. The major component of ox-LDL malondialdehyde-modified LDL (MDA-LDL) expresses on the surface of endothelial cells macrophages and smooth muscle cells. This oxidative process increases the atherogenic properties of LDL contributing to its accumulation in arterial walls.

Biological function summary

Oxidized phospholipids strongly influence inflammation and immune responses. These molecules act within cell membranes triggering toll-like receptors (TLRs) and other immune sensors. Ox-LDL forms part of immunoglobulin complexes and interacts with scavenger receptors like CD36 on macrophages. It recruits immune cells and promotes the release of cytokines perpetuating an inflammatory environment within blood vessels.

Pathways

Oxidized phospholipids participate in lipid oxidative stress and inflammatory pathways. They contribute significantly to the pathophysiology of the atherosclerotic process. In the oxidative stress pathways ox-LDL affects the balance of reactive oxygen species (ROS) and nitric oxide (NO) levels influencing vasodilation and endothelial function. It engages proteins such as apolipoprotein B-100 in the inflammatory process and connects to pathways involving NF-kB influencing various cellular responses.

Oxidized phospholipids relate mainly to cardiovascular diseases and atherosclerosis. The accumulation and interaction of ox-LDL with cells in the arterial wall drive plaque formation and instability which can lead to myocardial infarction. Additionally these modified lipids link with diabetes-related complications due to their role in promoting vascular inflammation. Proteins like lectin-like oxidized LDL receptor-1 (LOX-1) mediate ox-LDL uptake emphasizing its damaging role in metabolic syndromes.

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Product protocols

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Target data

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Publications (3)

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International journal of biological sciences 21:1275-1293 PubMed39897035

2025

Exosomal mediated lipid metabolism, ferritinophagy and CoQ-dependent pathway contributes to the ferroptosis of keratinocyte in SJS/TEN.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Pei Qiao,ChunYing Xiao,ZiPeng Cao,JiaoLing Chen,Hui Fang,JianKang Yang,ZeHua Kang,ErLe Dang,Shuai Shao,BingYu Pang,QingYang Li,ZhenLai Zhu,ShengXian Shen,Akito Hasegawa,Riichiro Abe,HongJiang Qiao,Gang Wang,Meng Fu

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2022

Advanced glycation end products promote osteoporosis by inducing ferroptosis in osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Ge,Jian Jie,Jie Yao,Wei Li,Yahui Cheng,Wenjuan Lu

Experimental and therapeutic medicine 21:250 PubMed33603858

2021

Correlations of cardiac function with inflammation, oxidative stress and anemia in patients with uremia.

Applications

Unspecified application

Species

Unspecified reactive species

Heping Zhang,Lei Fan,Huawei Liao,Lirong Tu,Jie Zhang,Dan Xu,Jiangchao Feng

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Oxidized LDL Assay Kit (MDA-LDL, Human)

ELISA - Oxidized LDL Assay Kit (MDA-LDL, Human) (AB242302)

ELISA - Oxidized LDL Assay Kit (MDA-LDL, Human) (AB242302)

Key facts

Detection method

Sample types

Reacts with

Sensitivity

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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