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AB133088

PAF Acetylhydrolase Assay Kit

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(3 Publications)

Abcam's PAF Acetylhydrolase Assay Kit (ab133088) provides an accurate and convenient method for measurement of PAF acetylhydrolase (PAF-AH) activity (both cytosolic and extracellular).

View Alternative Names

C14orf76, ASPG, 60 kDa lysophospholipase, LysoLP, Lysophospholipase-transacylase

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Other - PAF Acetylhydrolase Assay Kit (AB133088)
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Supplier Data

Other - PAF Acetylhydrolase Assay Kit (AB133088)

Activity of Human PAF-AH.

Representative Standard Curve using ab133088.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum, Cell Lysate

Assay type

Enzyme activity

Range

0.02 - 0.2 µmol/min/mL

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's PAF Acetylhydrolase Assay Kit (ab133088) provides an accurate and convenient method for measurement of PAF acetylhydrolase (PAF-AH) activity (both cytosolic and extracellular). The assay uses 2-thio PAF which serves as a substrate for all PAF-AHs. Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using 5,5'-dithio-bis-(2- nitrobenzoic acid).

Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of cells upon stimulation. PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). PAF-AHs are located intra- and extra-cellularly (for instance, in both cytosol and plasma) Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the sn-2 position and is associated with lipoproteins.

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Precision

[ { "reproducibilityType": "Inter", "sample": "PAF-AH", "replicates": 89, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "PAF-AH", "replicates": 89, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab133088-96Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"96-Well Plate Cover", "number":"AB133088-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PAF Acetylhydrolase Assay Buffer 1", "number":"AB133088-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"2-thio PAF (substrate)", "number":"AB133088-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human Plasma PAF-AH Standard", "number":"AB133088-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PAF Acetylhydrolase Assay Buffer 2", "number":"AB133088-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Solid Plate (Colorimetric Assay)", "number":"AB133088-CMP03", "productcode":"" }, { "size":"4 x 1 Vial", "name":"DTNB", "number":"AB133088-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities (By similarity). Can catalyze three types of transacylation reactions : (1) acyl transfer from 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to the sn-1(3) positions of glycerol and 2-acylglycerol (sn-1 to -1(3) transfer), (2) acyl transfer from 1-acyl-GPC to the sn-2 positions of 1-acyl-GPC, 1-acyl-sn-glycero-3-phosphoethanolamine (1-acyl-GPE), and other lysophospholipids (sn-1 to -2 transfer) and (3) acyl transfer from 2-acyl-GPC to the sn-1 position of 2-acyl-GPC and 2-acyl-GPE (sn-2 to -1 transfer) (By similarity). Mediates the synthesis of 1-arachidonoyl species of phospholipids by transferring the arachidonoyl residue from 2-arachidonoyl lysophospholipid to the sn-1 position of 2-acyl lysophospholipid (By similarity).
See full target information ASPG
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Antibodies (Basel, Switzerland) 14: PubMed40136470

2025

Development of Fully Human Antibodies Targeting SIRPα and PLA2G7 for Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Seungmin Shin,Du-San Baek,John W Mellors,Dimiter S Dimitrov,Wei Li

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 50:383-390 PubMed31755606

2019

Treatment with a platelet-activating factor receptor antagonist improves hemodynamics and reduces epinephrine requirements, in a lethal rodent model of anaphylactic shock.

Applications

Unspecified application

Species

Unspecified reactive species

Charles Tacquard,Walid Oulehri,Olivier Collange,Lene H Garvey,Susan Nicoll,Nicolas Tuzin,Bernard Geny,Paul M Mertes

PloS one 12:e0178174 PubMed28542249

2017

The human asparaginase enzyme (ASPG) inhibits growth in leukemic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Stefania Belviso,Rodolfo Iuliano,Rosario Amato,Nicola Perrotti,Miranda Menniti
View all publications
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