AB133091
PAF Acetylhydrolase Inhibitor Screening Assay Kit
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(1 Publication)
ab133091 uses 2-thio PAF as a substrate for PAF Acetylhydrolase (PAF-AH).
View Alternative Names
C14orf76, ASPG, 60 kDa lysophospholipase, LysoLP, Lysophospholipase-transacylase
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Supplier Data
Functional Studies - PAF Acetylhydrolase Inhibitor Screening Assay Kit (AB133091)
Inhibition of Human plasma PAF Acetylhydrolase by MAFP (IC50 = 250 nM).
Reactivity data
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Product details
ab133091 uses 2-thio PAF as a substrate for PAF Acetylhydrolase (PAF-AH). Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF Acetylhydrolase, free thiols are detected using 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB). The PAF Acetylhydrolase Inhibitor Screening assay includes Human plasma PAF Acetylhydrolase as control and is a time saving tool for screening vast numbers of inhibitors.
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
Product protocols
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Target data
Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities (By similarity). Can catalyze three types of transacylation reactions : (1) acyl transfer from 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to the sn-1(3) positions of glycerol and 2-acylglycerol (sn-1 to -1(3) transfer), (2) acyl transfer from 1-acyl-GPC to the sn-2 positions of 1-acyl-GPC, 1-acyl-sn-glycero-3-phosphoethanolamine (1-acyl-GPE), and other lysophospholipids (sn-1 to -2 transfer) and (3) acyl transfer from 2-acyl-GPC to the sn-1 position of 2-acyl-GPC and 2-acyl-GPE (sn-2 to -1 transfer) (By similarity). Mediates the synthesis of 1-arachidonoyl species of phospholipids by transferring the arachidonoyl residue from 2-arachidonoyl lysophospholipid to the sn-1 position of 2-acyl lysophospholipid (By similarity).
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Pharmaceutics 14: PubMed35214100
2022
Gallic Acid as a Non-Selective Inhibitor of α/β-Hydrolase Fold Enzymes Involved in the Inflammatory Process: The Two Sides of the Same Coin.
Applications
Unspecified application
Species
Unspecified reactive species
Marcos Hikari Toyama,Airam Rogero,Laila Lucyane Ferreira de Moraes,Gustavo Antônio Fernandes,Caroline Ramos da Cruz Costa,Mariana Novo Belchor,Agatha Manzi De Carli,Marcos Antônio de Oliveira
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