PAI1 Activity Assay Kit (Colorimetric)

The Plasminogen Activator Inhibitor-1 Activity Assay Kit is a two-step colorimetric assay. In the first step, samples are incubated with a known amount of tPA thereby allowing PAI-1 and tPA to form an inactive complex. In the second step, the residual free tPA converts plasminogen to plasmin, which in turn hydrolyzes the plasmin chromogenic substrate thereby releasing pNA (chromophore). The absorbance of the released pNA is inversely proportional to the PAI-1 activity in the samples. The assay kit is simple, specific and can detect as low as 2 U/ml of PAI-1 activity in samples.

This product is manufactured by BioVision, an Abcam company and was previously called K2040 Plasminogen Activator Inhibitor-1 Activity Assay Kit (C). K2040-100 is the same size as the 100 test size of ab283368.

Plasminogen Activator Inhibitor-1 (PAI-1), also known as endothelial plasminogen activator inhibitor, is a serine protease inhibitor that functions as the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA). PAI-1 is present in a number of tissues & cell types and is associated with various thrombotic and fibrinolytic complications. High level of PAI-1 activity is found in patients suffering from myocardial infarction, hemolytic uremic syndrome or stroke whereas low levels of PAI-1 activity are associated with bleeding disorders. Additionally, PAI-1 is a prognosticator in various cancers.