Paraoxonase 1 Activity Assay Kit ab241044 enables rapid measurement of Paraoxonase 1 (PON1) activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Nature communications 14:5572023Quantifying PON1 on HDL with nanoparticle-gated electrokinetic membrane sensor for accurate cardiovascular risk assessment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSonu Kumar,Nalin Maniya,Ceming Wang,Satyajyoti Senapati,Hsueh-Chia ChangPubMed 36732521
Key facts
- Detection method
Fluorescent
- Sample types
Plasma, Serum
Target data
Function
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
Alternative names
Serum paraoxonase/arylesterase 1, PON 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1, A-esterase 1, PON, PON1
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Paraoxonase 1 Activity Assay Kit ab241044 enables rapid measurement of Paraoxonase 1 (PON1) activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Serum paraoxonase/arylesterase 1, PON 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1, A-esterase 1, PON, PON1
Key facts
- Detection method
Fluorescent
- Sample types
Plasma, Serum
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Paraoxonase 1 Activity Assay Kit (ab241044) enables rapid measurement of Paraoxonase 1 (PON1) activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). This ensures dramatically greater sensitivity than UV or colorimetric assays and eliminates the need for dangerous toxic substrates. A selective PON1 inhibitor is provided for verification of PON1 specific activity. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 2.0 μU paraoxonase activity with a sample volume of 5 μL.
This product is manufactured by BioVision, an Abcam company and was previously called K999 Paraoxonase 1 (PON1) Activity Assay Kit (Fluorometric). K999-100 is the same size as the 100 test size of ab241044.
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3 product images
Functional Studies - Paraoxonase 1 Activity Assay Kit (ab241044)
Standard curve of PON1 Substrate metabolite fluorescence. One mole of fluorescence standard corresponds to the metabolism of one mole of PON1 Substrate.
Functional Studies - Paraoxonase 1 Activity Assay Kit (ab241044)
Reaction kinetics of PON1 Substrate metabolism in donor-pooled human serum (5 μL), donor-pooled human plasma (5 μL) and rabbit serum (2.5 μL) in the presence and absence of 200 μM of the selective PON1 inhibitor 2- hydroxyquinoline (no inhibitor conditions contained 0.4% DMSO as a solvent control).
Functional Studies - Paraoxonase 1 Activity Assay Kit (ab241044)
Quantification of PON1 activity in serum/plasma samples (mean ± SEM of four independent replicates). Assays were performed according to the kit protocol.
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