Paraoxonase 1 Activity Assay Kit ab241044 enables rapid measurement of Paraoxonase 1 (PON1) activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Fluorescent
Plasma, Serum
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
Serum paraoxonase/arylesterase 1, PON 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1, A-esterase 1, PON, PON1
Paraoxonase 1 Activity Assay Kit ab241044 enables rapid measurement of Paraoxonase 1 (PON1) activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Serum paraoxonase/arylesterase 1, PON 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1, A-esterase 1, PON, PON1
Fluorescent
Plasma, Serum
Microplate reader
Blue Ice
-20°C
-20°C
-20°C
Paraoxonase 1 Activity Assay Kit (ab241044) enables rapid measurement of Paraoxonase 1 (PON1) activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). This ensures dramatically greater sensitivity than UV or colorimetric assays and eliminates the need for dangerous toxic substrates. A selective PON1 inhibitor is provided for verification of PON1 specific activity. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 2.0 μU paraoxonase activity with a sample volume of 5 μL.
This product is manufactured by BioVision, an Abcam company and was previously called K999 Paraoxonase 1 (PON1) Activity Assay Kit (Fluorometric). K999-100 is the same size as the 100 test size of ab241044.
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Terms & Conditions.
Standard curve of PON1 Substrate metabolite fluorescence. One mole of fluorescence standard corresponds to the metabolism of one mole of PON1 Substrate.
Reaction kinetics of PON1 Substrate metabolism in donor-pooled human serum (5 μL), donor-pooled human plasma (5 μL) and rabbit serum (2.5 μL) in the presence and absence of 200 μM of the selective PON1 inhibitor 2- hydroxyquinoline (no inhibitor conditions contained 0.4% DMSO as a solvent control).
Quantification of PON1 activity in serum/plasma samples (mean ± SEM of four independent replicates). Assays were performed according to the kit protocol.
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