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AB241044

Paraoxonase 1 Activity Assay Kit

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(2 Publications)

Paraoxonase 1 Activity Assay Kit ab241044 enables rapid measurement of Paraoxonase 1 (PON1) activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm).

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

PON, PON1, Serum paraoxonase/arylesterase 1, PON 1, Aromatic esterase 1, K-45, Serum aryldialkylphosphatase 1, A-esterase 1

3 Images
Functional Studies - Paraoxonase 1 Activity Assay Kit (AB241044)
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Functional Studies - Paraoxonase 1 Activity Assay Kit (AB241044)

Quantification of PON1 activity in serum/plasma samples (mean ± SEM of four independent replicates). Assays were performed according to the kit protocol.

Functional Studies - Paraoxonase 1 Activity Assay Kit (AB241044)
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Functional Studies - Paraoxonase 1 Activity Assay Kit (AB241044)

Standard curve of PON1 Substrate metabolite fluorescence. One mole of fluorescence standard corresponds to the metabolism of one mole of PON1 Substrate.

Functional Studies - Paraoxonase 1 Activity Assay Kit (AB241044)
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Functional Studies - Paraoxonase 1 Activity Assay Kit (AB241044)

Reaction kinetics of PON1 Substrate metabolism in donor-pooled human serum (5 μL), donor-pooled human plasma (5 μL) and rabbit serum (2.5 μL) in the presence and absence of 200 μM of the selective PON1 inhibitor 2- hydroxyquinoline (no inhibitor conditions contained 0.4% DMSO as a solvent control).

Key facts

Detection method

Fluorescent

Sample types

Plasma, Serum

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Paraoxonase 1 Activity Assay Kit (ab241044) enables rapid measurement of Paraoxonase 1 (PON1) activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). This ensures dramatically greater sensitivity than UV or colorimetric assays and eliminates the need for dangerous toxic substrates. A selective PON1 inhibitor is provided for verification of PON1 specific activity. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 2.0 uU paraoxonase activity with a sample volume of 5 uL.

Other Notes
This product was previously called K999 Biovision Paraoxonase 1 (PON1) Activity Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab241044-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"PON1 Substrate", "number":"AB241044-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PON1 Inhibitor", "number":"AB241044-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Paraoxonase Positive Control", "number":"AB241044-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Paraoxonase Assay Buffer", "number":"AB241044-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Fluorescence Standard III", "number":"AB241044-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
See full target information PON1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39408985

2024

Paraoxonase-1 as a Cardiovascular Biomarker in Caribbean Hispanic Patients Treated with Clopidogrel: Abundance and Functionality.

Applications

Unspecified application

Species

Unspecified reactive species

Mariangeli Monero-Paredes,Ednalise Santiago,Kelvin Carrasquillo-Carrion,Jessicca Y Renta,Igor B Rogozin,Abiel Roche-Lima,Jorge Duconge

Nature communications 14:557 PubMed36732521

2023

Quantifying PON1 on HDL with nanoparticle-gated electrokinetic membrane sensor for accurate cardiovascular risk assessment.

Applications

Unspecified application

Species

Unspecified reactive species

Sonu Kumar,Nalin Maniya,Ceming Wang,Satyajyoti Senapati,Hsueh-Chia Chang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com