PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase)
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(3 Publications)
- FuncS
Supplier Data
Functional Studies - PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase) (AB239714)
Phosphoenolpyruvate Carboxykinase (PEPCK) kinetic activity measured in lysates : HEK-293 cells (66 µg), rat liver (223 μg) and rat kidney (145 µg)
- FuncS
Supplier Data
Functional Studies - PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase) (AB239714)
Pyruvate standard curve
- FuncS
Supplier Data
Functional Studies - PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase) (AB239714)
PEPCK Specific Activity in biological samples
Reactivity data
Product details
PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase) (ab239714) provides a quick and easy way for measuring PEPCK activity in various samples.
In this assay, Phosphoenolpyruvate Carboxykinase is coupled with a set of enzymes that convert PEP and carbonate into a series of intermediates and hydrogen peroxide, which in turn, reacts with a probe and converted generating a colorimetric signal (OD: 570 nm). The color intensity is directly proportional to the amount of active Phosphoenolpyruvate Carboxykinase present in samples.
The assay is simple, sensitive, high-throughput adaptable and can detect less than 10 μU of Phosphoenolpyruvate Carboxykinase activity per sample.
This product is manufactured by BioVision, an Abcam company and was previously called K359 Phosphoenolpyruvate Carboxykinase Activity Assay Kit (Colorimetric). K359-100 is the same size as the 100 test size of ab239714.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PEPCK facilitates the metabolic pathway by converting oxaloacetate into phosphoenolpyruvate a critical step in gluconeogenesis. It exists in two forms: cytosolic PEPCK-C and mitochondrial PEPCK-M allowing the enzyme to operate in distinct cellular compartments. It does not form a complex with other proteins but it does have a significant role in enabling the synthesis of glucose from non-carbohydrate sources an essential process in energy metabolism.
Pathways
PEPCK participates in gluconeogenesis and the tricarboxylic acid cycle. It interacts with enzymes such as pyruvate carboxylase which provides oxaloacetate as a substrate in gluconeogenesis. The PEP carboxykinase reaction is central to carbohydrate metabolism linking anabolic and catabolic pathways and ensuring that cellular energy demands are met under varying conditions.
Product protocols
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Publications (3)
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Oncogenesis 12:35 PubMed37407566
2023
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Biosensors 12: PubMed35884248
2022
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Molecules (Basel, Switzerland) 26: PubMed34946711
2021
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