Pepsin/Pepsinogen Assay Kit (Fluorometric)
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(1 Publication)
Pepsin/Pepsinogen Assay Kit (Fluorometric) (ab238722) is a homogenous assay that allows for quantification of pepsin activity in gastric tissues (stomach, duodenum etc.
View Alternative Names
Pepsin A-4, Pepsinogen-4, PGA4, Pepsin A-5, Pepsinogen-5, PGA5, Pepsin A-3, Pepsinogen-3, PGA3
- Biochemical assay
Supplier Data
Biochemical assay - Pepsin/Pepsinogen Assay Kit (Fluorometric) (AB239722)
Estimation of pepsinogen activity in pooled normal human serum and single-donor serum from a gastric ulcer patient with confirmed H. Pylori infection (each 10 μl of undiluted serum).
Data are mean ± SEM of 3 replicates, assayed according to the kit protocol.
- Biochemical assay
Supplier Data
Biochemical assay - Pepsin/Pepsinogen Assay Kit (Fluorometric) (AB239722)
Kinetics of Pepsin Substrate metabolism by porcine gastric mucosal pepsin (3.33 ng purified enzyme) and specificity of substrate metabolism by pepsin versus other aspartic proteases.
The acid-activated proteases Cathepsin D and E exhibit minimal assay interference, even when present at ≥300-fold excess by mass.
- Biochemical assay
Supplier Data
Biochemical assay - Pepsin/Pepsinogen Assay Kit (Fluorometric) (AB239722)
MCA standard curve.
One mole of MCA corresponds to the cleavage of one mole of fluorogenic Pepsin Substrate
Reactivity data
Product details
Pepsin/Pepsinogen Assay Kit (Fluorometric) (ab238722) is a homogenous assay that allows for quantification of pepsin activity in gastric tissues (stomach, duodenum etc.) and various biological fluids (serum/plasma, gastric juice, vomit). The assay utilizes a synthetic peptide substrate bearing both a fluorophore and a fluorescence quencher. Upon cleavage by pepsin, the fluorophore-bearing peptide fragment is unquenched to produce a bright fluorescent signal (Ex/Em = 328/418 nm). Lysosomal aspartic proteases in the peptidase A1 family (Cathepsin D and E) do not interfere with the assay. The assay is rapid, simple to perform and is vastly more sensitive than the classical hemoglobin degradation assay, with a detection limit of 500 μU pepsin activity per well.
This product is manufactured by BioVision, an Abcam company and was previously called K446 Pepsin/Pepsinogen Activity Assay Kit (Fluorometric). K446-100 is the same size as the 100 test size of ab239722.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Pepsin fulfills an important role in the digestive process by breaking down larger protein molecules into smaller peptides facilitating nutrient absorption. It does not form part of a larger protein complex but operates independently within the gastric environment. Pepsin's enzymatic action targets specific peptide bonds ensuring efficient degradation of dietary proteins into absorbable forms. The specificity of its substrate binding ensures effective digestion and subsequent utilization of amino acids.
Pathways
Pepsin's activity integrates into proteolytic pathways particularly the catabolic processes in the gastrointestinal tract. It interacts with other digestive enzymes including trypsin and chymotrypsin which further hydrolyze peptides originated by pepsin. These interactions highlight its seamless fit in the digestive enzyme cascade optimized for effective protein turnover and nutrient release into the body. The pepsin pathway coordinates closely with the gastric and duodenal enzymatic processes.
Product protocols
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Target data
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Publications (1)
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Scientific reports 11:17071 PubMed34426628
2021
Applications
Unspecified application
Species
Unspecified reactive species
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