AB150680

Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)

Name: Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (ab150680) | Abcam
Brand: Abcam Limited
SKU: AB150680
Price: 150 USD
Availability: InStock

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(126 Publications)

The Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) ab150680 is used to stain lymphocytes and mucopolysaccharides/glycosaminoglycans (GAGs) in tissue sections.

- Highly specific; selectively stains neutral mucins, enabling differentiation between different mucin types within a tissue sample
- Can be also used for the demonstration of fungal organisms in tissue sections
- Cited in over 90 publications

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (AB150680)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (AB150680)

ab150680 Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) staining formalin-fixed-paraffin embedded human kidney. Showing fungal organisms (magenta), PAS Positive Material (Magenta), Nuclei (Black/Blue) and other tissue components (Green/Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (AB150680)

Staining of Intestine using ab150680 - Periodic Acid Schiff (PAS) Stain Kit. Showing fungal organisms (magenta), PAS Positive Material (Magenta), Nuclei (Black/Blue) and other tissue components (Green/Blue).

Key facts

Sample types

Tissue sections

Assay time

45m

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Product details

How the assay works

In Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) protocol, tissue carbohydrates are oxidized by periodic acid forming aldehydes capable of binding with Schiff’s Solution. Visualization of Schiff’s is caused by a restoration of the dye’s quinoid structure resulting in characteristic magenta staining. Light green provides a contrasting counterstain. The staining pattern of the lymphocytes are helpful in making therapeutic decisions in established cases of lymphocytic leukemia.

Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) protocol summary:

- deparaffinize sections (if necessary) and hydrate
- immerse slide in PAS solution for 5-10 min
- rinse 4 times with water
- immerse slide in Schiff's solution for 15-30 min and rinse
- stain slide with Hematoxylin for 2-3 min and rinse
- incubate in Bluing reagent for 30 s and rinse
- incubate in Light Green Solution for 2 min and rinse
- dehydrate with alcohol, clear and mount

Staining Interpretation

Fungal Organisms Magenta
PAS Positive Material Magenta
Nuclei Black/Blue
Other Tissue Components Green/Blue
Control Tissue: Kidney, Intestine, Liver.

For the demonstration of fungal organisms in tissue sections, we recommend PAS Stain Kit (Fungus, Periodic Acid Schiff Stain) ab245886.

How other researchers are using

Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) has been used in a variety of sample type including:
- Mouse Liver tissues 1
- Mouse kidney tissues 2
- Human cornea 3

References:
1 - Liu Z et al. 2023; 2 - Meng Q et al. 2023; 3 - Kim H et al. 2023.

Related and recommended products

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Other Notes

Please note, the 125 mL selling size, was previously described as 100 Test. There has been no change to the physical product.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lymphocytes are critical cells of the immune system known for their roles in adaptive immunity. They express a variety of receptors including T-cell receptors and B-cell receptors on their surface which allow them to recognize antigens. These receptors have different masses depending on the specific type. Lymphocytes primarily reside in the blood lymphoid organs like the spleen and lymph nodes and mucosal tissues. Mucopolysaccharides also known as glycosaminoglycans are long chains of sugar molecules found throughout the body including on cell surfaces and in the extracellular matrix. They vary in mass depending on their structure and their main role is to provide structural support and help in cell signaling.

Biological function summary

Lymphocytes play essential roles in the immune response by recognizing and attacking foreign invaders such as viruses and bacteria. They are not part of a larger complex but function through interactions with other immune cells. Mucopolysaccharides contribute to maintaining the structural integrity and fluidity of tissues. They support cell adhesion and communication create hydrophilic environments and are involved in cell migration. In histology PAS staining is a common technique to identify mucopolysaccharides as they stain positively highlighting the presence of these molecules as PAS positive material due to the PAS stain color.

Pathways

Lymphocytes are central to immune system pathways particularly the adaptive immune response and the lymphoid signaling pathway. For the adaptive immune response they interact with proteins such as CD4 and CD8 essential in T-cell function. In the lymphoid signaling pathway they transmit signals that lead to immune activation and regulation. Mucopolysaccharides participate in signaling pathways like those involving transforming growth factor-beta (TGF-beta) where they mediate cell growth and differentiation.

Lymphocytes are a focus in conditions such as autoimmune diseases and lymphomas. Aberrations in their function can result in autoimmune disorders where the immune system attacks self-tissues. Lymphomas involve malignant transformation of lymphocytes leading to cancer of the lymphatic system. In these contexts proteins such as interleukins and tumor necrosis factor are relevant. Mucopolysaccharides are involved in disorders like mucopolysaccharidoses a group of metabolic disorders caused by enzyme deficiencies leading to the accumulation of these compounds. Proteins such as enzymes responsible for their breakdown like iduronate sulfatase are closely connected in the metabolic pathways disrupted in these diseases.

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Product protocols

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Target data

See full target information Orthoperiodic acid

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Publications (126)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:1555-1566 PubMed40610751

2025

The ubiquitin E3 ligase TRIM21 suppresses type I interferon signaling via STING degradation and ameliorates systemic autoimmunity.

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Da Som Kim,Youngjae Park,George C Tsokos,Mi-La Cho,Seung-Ki Kwok

Cell communication and signaling : CCS 23:257 PubMed40450320

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The E3 ubiquitin ligase MARCH2 controls TNF-α mediated inflammation by autoubiquitination.

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Kiramage Chathuranga,Pramodya Rathnapala,Asela Weerawardhana,Tae-Hwan Kim,Yebin Seong,W A Gayan Chathuranga,Ashan Subasinghe,D K Haluwana,Nuwan Gamage,Youn Jung Choi,Jae U Jung,Jong-Soo Lee

Stem cell research & therapy 16:187 PubMed40251601

2025

Chemical reprogramming culture for the expansion of salivary gland epithelial basal progenitor cells.

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Ye Jin Jeong,Yongpyo Hong,Yeo-Jun Yoon,Nam Suk Sim,Seung-Min Hong,Jae-Yol Lim

American journal of translational research 17:528-537 PubMed39959245

2025

The molecular mechanism underlying angiogenesis in a mouse model of chronic kidney disease after ischemic stroke.

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Fang Wang,Liangxiang Lu,Jialun Feng,Jinhua Zheng,Zefeng Wei,Ziqiang Wang

Allergy, asthma & immunology research 17:77-93 PubMed39895604

2025

Inotodiol Attenuates Mucosal Inflammation in a Mouse Model of Eosinophilic Chronic Rhinosinusitis.

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Jaein Chung,Se Yeon Im,Soo-Kyoung Park,Da Beom Heo,Han Wool John Sung,Danielle Ohm,Eun Hee Chung,Jong-Tae Park,Yong Min Kim

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Therapeutic potential of 4-hexylresorcinol in reducing sarcopenia in diabetic masseter muscle.

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Dhouha Gaida,Young-Wook Park,Yei-Jin Kang,Seong-Gon Kim

Journal of agricultural and food chemistry 73:1231-1243 PubMed39754576

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Feruloylacetone and Its Analog Demethoxyferuloylacetone Mitigate Obesity-Related Muscle Atrophy and Insulin Resistance in Mice.

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Yen-Chun Koh,Han-Wen Hsu,Pin-Yu Ho,Wei-Sheng Lin,Kai-Yu Hsu,Anju Majeed,Chi-Tang Ho,Min-Hsiung Pan

Nature communications 16:233 PubMed39747843

2025

Sticky organisms create underwater biological adhesives driven by interactions between EGF- and GlcNAc- containing polysaccharides.

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Jimin Choi,Seunghyeon Lee,Yongjin Lee,Dong Soo Hwang

Cellular and molecular life sciences : CMLS 82:13 PubMed39719538

2024

Trimethylamine-N-oxide accelerates osteoporosis by PERK activation of ATF5 unfolding.

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Yu-Han Lin,Wei-Shiung Lian,Re-Wen Wu,Yu-Shan Chen,Shin-Long Wu,Jih-Yang Ko,Shao-Yu Wang,Holger Jahr,Feng-Sheng Wang

International journal of molecular sciences 25: PubMed39596347

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4-Hexylresorcinol Enhances Glut4 Expression and Glucose Homeostasis via AMPK Activation and Histone H3 Acetylation.

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Xiangguo Che,Ji-Hyeon Oh,Yei-Jin Kang,Dae-Won Kim,Seong-Gon Kim,Je-Yong Choi,Umberto Garagiola

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Product promise

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Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (AB150680)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (AB150680)

Key facts

Sample types

Assay time

Complementary Products

Secondary Antibodies

Assay Kits

Assay Kits

Primary Antibodies

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (126)

Product promise