Peroxidase Assay Kit ab155895 provides a convenient colorimetric and fluorometric means to measure peroxidase activity in biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- International journal of molecular sciences 24:2023Role of Tyrosine Nitrosylation in Stress-Induced Major Depressive Disorder: Mechanisms and Implications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGregory C Wilson,Simone Keitsch,Matthias Soddemann,Barbara Wilker,Michael J Edwards,Erich GulbinsPubMed 37834072
- BioMed research international 2022:52662112022Light Emitting Diode Photobiomodulation Enhances Oxidative Redox Capacity in Murine Macrophages Stimulated with Venom and Isolated PLAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValdison Pereira Dos Reis,Sulamita da Silva Setúbal,Alex A Ferreira E Ferreira,Hallison Mota Santana,Milena Daniela Souza Silva,Ortência De Oliveira Sousa,Charles Nunes Boeno,Andreimar M Soares,Stella R Zamuner,Juliana P ZulianiPubMed 35872869
- Microorganisms 10:2022Suppressive Effect of Bioactive Extracts of sp. H8-1 and sp. K203 on Tomato Wilt Caused by subsp. .Applications:Unspecified applicationReactive species:Unspecified reactive speciesHwajin Jang,Sang Tae Kim,Mee Kyung SangPubMed 35208859
Key facts
- Detection method
- Colorimetric/Fluorometric
- Sample types
- Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Enzyme activity
- Reactive species
- Mammals
What's included?
Recommended products
Peroxidase Assay Kit ab155895 provides a convenient colorimetric and fluorometric means to measure peroxidase activity in biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric/Fluorometric
- Sample types
- Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Peroxidase Assay Kit ab155895 provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the red fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
Other Notes
This product was previously called K772 Peroxidase Activity Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ to ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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2 product images
Functional Studies - Peroxidase Activity Assay Kit (ab155895)
H2O2 standard curve - fluorometric. An example of a Fluorimetric Standard Curve
Functional Studies - Peroxidase Activity Assay Kit (ab155895)
H2O2 Standard Curve - Colorimetric. An example of a Colorimetric Standard Curve
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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