Peroxidase Activity Assay Kit
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(3 Publications)
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
- FuncS
Supplier Data
Functional Studies - Peroxidase Activity Assay Kit (AB155895)
H2O2 Standard Curve - Colorimetric. An example of a Colorimetric Standard Curve
- FuncS
Supplier Data
Functional Studies - Peroxidase Activity Assay Kit (AB155895)
H2O2 standard curve - fluorometric. An example of a Fluorimetric Standard Curve
- Other
Supplier Data
Other - Peroxidase Activity Assay Kit (AB155895)
Representative image of Peroxidase Activity Assay Kit ab155895
Components shown from left to right :
- Developer Solution V
- OxiRed Probe
- H2O2 Standard
- Assay Buffer 18
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Complementary Products
Reactivity data
Product details
Peroxidase Assay Kit ab155895 provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the red fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
Other Notes
This product was previously called K772 Peroxidase Activity Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ to ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
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Target data
Publications (3)
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International journal of molecular sciences 24: PubMed37834072
2023
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BioMed research international 2022:5266211 PubMed35872869
2022
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Unspecified reactive species
Microorganisms 10: PubMed35208859
2022
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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