Peroxidase Assay Kit (ab324119) uses a one-step, homogeneous, no wash, luminescent-readout assay method.
Application | Reactivity | Dilution info | Notes |
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Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
HPRC1, PRXC1A, Peroxidase C1A
Peroxidase Assay Kit (ab324119) uses a one-step, homogeneous, no wash, luminescent-readout assay method.
Peroxidase Assay Kit (ab324119) uses a one-step, homogeneous, no wash, luminescent-readout assay method.
The assay kit contains hydrogen peroxide and an assay buffer containing a chemiluminescent reagent. The chemiluminescent reagent becomes luminescent in a reaction coupled with the action of peroxidases on hydrogen peroxide.
In the peroxidase assay protocol, the working solution is added to samples, with a 30-min to 2-hr incubation and monitoring of luminescence intensity using a luminometric plate reader.
HRP (horseradish peroxidase) is used to produce a standard curve.
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HRP dose response was measured with Peroxidase Assay Kit (Luminometric) in a solid black 384-well plate using a NOVOstar plate reader (BMG Labtech).
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