Phagocytosis Assay Kit (Green E. coli) (ab235900) uses heat-killed, fluorescently pre-labeled E. coli particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Journal of neurochemistry 165:29-542023Microglial reprogramming by Hv1 antagonism protects neurons from inflammatory and glutamate toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiego R Hernandez-Espinosa,Jenna R Gale,Mia G Scrabis,Elias AizenmanPubMed 36625847
- Frontiers in immunology 13:10120782022Acid external and internal environment exchange the tissue immune gene expression compared to the mouse macrophage polarization model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPo-Kai Pan,Tsung-Meng Wu,Hsin-Yuan Tsai,I-Cheng Cho,Hsin-Wei Tseng,Tai-Du Lin,Fan-Hua Nan,Yu-Sheng WuPubMed 36225935
- Molecular medicine (Cambridge, Mass.) 28:1042022Protective effect of α7 nicotinic acetylcholine receptor activation on experimental colitis and its mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyuan Pu,Zhenzi Su,Junaid Wazir,Chen Zhao,Lulu Wei,Ranran Wang,Qiyi Chen,Saifang Zheng,Shaoyi Zhang,Hongwei WangPubMed 36058917
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
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Phagocytosis Assay Kit (Green E. coli) (ab235900) uses heat-killed, fluorescently pre-labeled E. coli particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay Platform
Microplate reader, Fluor. microscope, Flow cyt.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Phagocytosis Assay Kit (Green E. coli) (ab235900) utilizes heat-killed, fluorescently pre-labeled E. coli particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptor (TLR) ligands.
This product is manufactured by BioVision, an Abcam company and was previously called K963 EZCell™ Phagocytosis Assay Kit (Green E. coli). K963-100 is the same size as the 100 test size of ab235900.
Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. E. coli and other bacterial strains are often used as a pathogen in phagocytosis assays.
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3 product images
Flow Cytometry - Phagocytosis Assay Kit (Green E.coli) (ab235900)
Flow cytometry plot.
J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 hour at 37°C prior to addition of 5 μL of E. coli particles. Phagocytosis was conducted for 2 hours and the amount of engulfed E. coli was determined as described in the Assay Protocol. Blue line: untreated control cells; green line: macrophages with engulfed E. coli particles; violet line: inhibition of phagocytosis by Cytochalasin D.
Functional Studies - Phagocytosis Assay Kit (Green E.coli) (ab235900)
Inhibition of phagocytosis.
J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 hour at 37°C prior to addition of 5 μL of E. coli particles. Phagocytosis was conducted for 2 hours and the amount of engulfed E. coli was determined as described in the Assay Protocol. Panel A and B: images of nontreated cells. Panel C and D: treatment with Cytochalasin D.
Functional Studies - Phagocytosis Assay Kit (Green E.coli) (ab235900)
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