AB234053

Phagocytosis Assay Kit (Green Zymosan)

Name: Phagocytosis Assay Kit (Green Zymosan) (ab234053) | Abcam
Brand: Abcam Limited
SKU: AB234053
Price: 500 USD
Availability: InStock

Be the first to review this product! Submit a review

(9 Publications)

Phagocytosis Assay Kit (Green Zymosan) (ab234053) uses Zymosan particles, that are fluorescently labeled with a green dye, to detect and quantify in vitro phagocytosis by fluorescent microscope, fluorometric plate reader or flow cytometry.

- Ex/Em 490/520 nm
- Validated as macrophage phagocytosis assay.
- includes labeled Zymosan particles and assay buffer. Also includes quenching solution for readout on microscope / plate reader.

3 Images

Functional Studies - Phagocytosis Assay Kit (Green Zymosan) (AB234053)

FuncS

Supplier Data

Functional Studies - Phagocytosis Assay Kit (Green Zymosan) (AB234053)

Inhibition of phagocytosis.

J774 macrophages were seeded overnight at 5 x 10⁵ of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 h at 37°C prior to addition of 5 μL of Zymosan particles. Phagocytosis was conducted for 2 hours and the amount of engulfed Zymosan was determined as described in the Assay Protocol. Inhibition of phagocytosis. Panel A and B : images of non-treated cells. Panel C and D : treatment with Cytochalasin D.

FuncS

Supplier Data

Functional Studies - Phagocytosis Assay Kit (Green Zymosan) (AB234053)

J774 macrophages were seeded overnight at 5 x 10⁵ of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 h at 37°C prior to addition of 5 μL of Zymosan particles. Phagocytosis was conducted for 2 hours and the amount of engulfed Zymosan was determined as described in the Assay Protocol. Flow cytometry plot. Red line : untreated control cells; green line : macrophages with engulfed Zymosan particles; blue line : inhibition of phagocytosis by Cytochalasin D.

FuncS

Supplier Data

Functional Studies - Phagocytosis Assay Kit (Green Zymosan) (AB234053)

Zymosan Standard curve

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay Platform

Microplate reader, Fluor. microscope, Flow cyt.

style

left-column

style

left-column

Product details

Phagocytosis Assay Kit (Green Zymosan) (ab234053) uses pre-labeled Zymosan particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptors ligands (TLR).

Zymosan phagocytosis assay protocol summary
- add Green Zymosan reagent to cells, and incubate for 2-3 hours
- harvest cells by centrifugation, remove supernatant, and resuspend cells in Assay Buffer; repeat wash step 3 times
- for fluorescent microscope or fluorometric plate reader analysis, use Quenching Solution
- analyze cells with flow cytometer, fluorescent microscope, or fluorometric plate reader

Related phagocytosis assays
Phagocytosis Assay Kit (Green E.coli) ab235900
Phagocytosis Assay Kit (Red E. coli) ab235901
Phagocytosis Assay Kit (Red Zymosan) ab234054
Phagocytosis Assay Kit (Zymosan, Colorimetric) ab211156

Other notes
This product was previously called K397 Biovision EZCell™ Phagocytosis Assay Kit (Green Zymosan). Biovision was acquired by Abcam in 2021.

Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae), and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style

left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab234053-100Test", "assetComponentDetails": [ { "size":"1 x 0.5 mL", "name":"Green Zymosan", "number":"AB234053-CMP02", "productcode":"" }, { "size":"2 x 1 mL", "name":"Buffer Additive Solution", "number":"AB234053-CMP03", "productcode":"" }, { "size":"2 x 100 mL", "name":"Assay Buffer 27", "number":"AB234053-CMP04", "productcode":"" }, { "size":"1 x 0.5 mL", "name":"10X Quenching Solution I", "number":"AB234053-CMP01", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Please refer to protocols

style

left-column

style

left-column

Product protocols

style

left-column

Target data

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39519098

2024

Pancreatic Adenocarcinoma Up-Regulated Factor (PAUF) Transforms Human Monocytes into Alternative M2 Macrophages with Immunosuppressive Action.

Applications

Unspecified application

Species

Unspecified reactive species

Yeon Jeong Kim,Sitansu Sekhar Nanda,Fen Jiang,Seung Yeon Pyo,Jin-Yeong Han,Sang Seok Koh,Tae Heung Kang

Aging cell 23:e14133 PubMed38459711

2024

Chronic TNF exposure induces glucocorticoid-like immunosuppression in the alveolar macrophages of aged mice that enhances their susceptibility to pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine L Kruckow,Elizabeth Murray,Elnur Shayhidin,Alexander F Rosenberg,Dawn M E Bowdish,Carlos J Orihuela

Infection and immunity 92:e0050423 PubMed38193711

2024

IFN-λ3 is induced by and can inhibit parasite growth in cell line models but not in the mouse model, while it shows a significant association with leishmaniasis in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Manjarika De,Soumi Sukla,Seema Bharatiya,Sagar Keshri,Debarati Guha Roy,Sutopa Roy,Debrupa Dutta,Shriya Saha,Sarfaraz Ahmad Ejazi,V Ravichandiran,Nahid Ali,Mitali Chatterjee,Sreedhar Chinnaswamy

Investigative ophthalmology & visual science 64:7 PubMed37145604

2023

Microglial CD11b Knockout Contributes to Axonal Debris Clearance and Axonal Degradation Attenuation via IGF-1 After Acute Optic Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxing Zhou,Sen Lin,Qiumei Hu,Xue Li,Xi Chen,Linlin Luo,Shiyang Ye,Wei Liu,Jian Ye

Proceedings of the National Academy of Sciences of the United States of America 120:e2215005120 PubMed37126685

2023

CRISPR editing demonstrates rs10490924 raised oxidative stress in iPSC-derived retinal cells from patients with -related AMD.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ju Chang,Laura A Jenny,Yong-Shi Li,Xuan Cui,Yang Kong,Yao Li,Janet R Sparrow,Stephen H Tsang

Frontiers in immunology 13:1095879 PubMed36713449

2023

Placental macrophages present distinct polarization pattern and effector functions depending on clinical onset of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Horvat Mercnik,Carolin Schliefsteiner,Herbert Fluhr,Christian Wadsack

Acta pharmacologica Sinica 43:2253-2266 PubMed35132190

2022

CB2 receptor activation inhibits the phagocytic function of microglia through activating ERK/AKT-Nurr1 signal pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Wen Han,Qian-Hang Shao,Xiao-Tong Wang,Kai-Li Ma,Nai-Hong Chen,Yu-He Yuan

Journal of immunology (Baltimore, Md. : 1950) 207:2589-2597 PubMed34625522

2021

Atopic Neutrophils Prevent Postviral Airway Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Syed-Rehan A Hussain,Michelle Rohlfing,Jenny Resiliac,Jennifer Santoro,Mark E Peeples,Dominique Garcin,Mitchell H Grayson

Journal of inflammation research 14:1973-1989 PubMed34045879

2021

Urinary Trypsin Inhibitor Protects Tight Junctions of Septic Pulmonary Capillary Endothelial Cells by Regulating the Functions of Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijie Wang,Wenliang Song,Chengyuan Xie,Wenhong Zhong,Hui Xu,Qiuping Zhou,Yiyu Deng,Yimei Hong,Xin Li,Ming Fang

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Phagocytosis Assay Kit (Green Zymosan)

Functional Studies - Phagocytosis Assay Kit (Green Zymosan) (AB234053)

Functional Studies - Phagocytosis Assay Kit (Green Zymosan) (AB234053)

Functional Studies - Phagocytosis Assay Kit (Green Zymosan) (AB234053)

Key facts

Detection method

Sample types

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Primary Antibodies

Assay Kits

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (9)

Product promise