Phagocytosis Assay Kit (Green Zymosan) (ab234053) uses pre-labeled Zymosan particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Investigative ophthalmology & visual science 64:72023Microglial CD11b Knockout Contributes to Axonal Debris Clearance and Axonal Degradation Attenuation via IGF-1 After Acute Optic Nerve Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaxing Zhou,Sen Lin,Qiumei Hu,Xue Li,Xi Chen,Linlin Luo,Shiyang Ye,Wei Liu,Jian YePubMed 37145604
- Proceedings of the National Academy of Sciences of the United States of America 120:e22150051202023CRISPR editing demonstrates rs10490924 raised oxidative stress in iPSC-derived retinal cells from patients with -related AMD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYa-Ju Chang,Laura A Jenny,Yong-Shi Li,Xuan Cui,Yang Kong,Yao Li,Janet R Sparrow,Stephen H TsangPubMed 37126685
- Frontiers in immunology 13:10958792023Placental macrophages present distinct polarization pattern and effector functions depending on clinical onset of preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonika Horvat Mercnik,Carolin Schliefsteiner,Herbert Fluhr,Christian WadsackPubMed 36713449
- Acta pharmacologica Sinica 43:2253-22662022CB2 receptor activation inhibits the phagocytic function of microglia through activating ERK/AKT-Nurr1 signal pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi-Wen Han,Qian-Hang Shao,Xiao-Tong Wang,Kai-Li Ma,Nai-Hong Chen,Yu-He YuanPubMed 35132190
- Journal of immunology (Baltimore, Md. : 1950) 207:2589-25972021Atopic Neutrophils Prevent Postviral Airway Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSyed-Rehan A Hussain,Michelle Rohlfing,Jenny Resiliac,Jennifer Santoro,Mark E Peeples,Dominique Garcin,Mitchell H GraysonPubMed 34625522
- Journal of inflammation research 14:1973-19892021Urinary Trypsin Inhibitor Protects Tight Junctions of Septic Pulmonary Capillary Endothelial Cells by Regulating the Functions of Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuijie Wang,Wenliang Song,Chengyuan Xie,Wenhong Zhong,Hui Xu,Qiuping Zhou,Yiyu Deng,Yimei Hong,Xin Li,Ming FangPubMed 34045879
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Reactive species
Mammals
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Phagocytosis Assay Kit (Green Zymosan) (ab234053) uses pre-labeled Zymosan particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Reactive species
Mammals
- Assay Platform
Microplate reader, Fluor. microscope, Flow cyt.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Phagocytosis Assay Kit (Green Zymosan) (ab234053) utilizes pre-labeled Zymosan particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptors ligands (TLR).
This product is manufactured by BioVision, an Abcam company and was previously called K397 EZCell™ Phagocytosis Assay Kit (Green Zymosan). K397-100 is the same size as the 100 test size of ab234053.
Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae), and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays.
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3 product images
Functional Studies - Phagocytosis Assay Kit (Green Zymosan) (ab234053)
J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 h at 37°C prior to addition of 5 μL of Zymosan particles. Phagocytosis was conducted for 2 hours and the amount of engulfed Zymosan was determined as described in the Assay Protocol. Inhibition of phagocytosis. Panel A and B: images of non-treated cells. Panel C and D: treatment with Cytochalasin D.
Functional Studies - Phagocytosis Assay Kit (Green Zymosan) (ab234053)
J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 h at 37°C prior to addition of 5 μL of Zymosan particles. Phagocytosis was conducted for 2 hours and the amount of engulfed Zymosan was determined as described in the Assay Protocol. Flow cytometry plot. Red line: untreated control cells; green line: macrophages with engulfed Zymosan particles; blue line: inhibition of phagocytosis by Cytochalasin D.
Functional Studies - Phagocytosis Assay Kit (Green Zymosan) (ab234053)
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