Phagocytosis Assay Kit ab235901 uses heat-killed, fluorescently pre-labeled E. coli particles as a tool for quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry (540 / 570 nm).
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Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
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Phagocytosis Assay Kit ab235901 uses heat-killed, fluorescently pre-labeled E. coli particles as a tool for quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry (540 / 570 nm).
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay Platform
Microplate reader, Fluor. microscope, Flow cyt.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Phagocytosis Assay Kit (Red E. coli) (ab235901) utilizes heat-killed, fluorescently pre-labeled E. coli particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptor (TLR) ligands.
This product is manufactured by BioVision, an Abcam company and was previously called K964 EZCell™ Phagocytosis Assay Kit (Red E. coli). K964-100 is the same size as the 100 test size of ab235901.
Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. E. coli and other bacterial strains are often used as a pathogen in phagocytosis assays.
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3 product images
Flow Cytometry - Phagocytosis Assay Kit (Red E. coli) (ab235901)
J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 hour at 37°C prior to addition of 5 μL of E. coli particles. Phagocytosis was conducted for 2 hours and the amount of engulfed E. coli was determined as described in the Assay Protocol. Blue line: untreated control cells; red line: macrophages with engulfed E. coli particles; green line: inhibition of phagocytosis by Cytochalasin D.
Functional Studies - Phagocytosis Assay Kit (Red E. coli) (ab235901)
Inhibition of phagocytosis. J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 hour at 37°C prior to addition of 5 μL of E. coli particles. Phagocytosis was conducted for 2 hours and the amount of engulfed E. coli was determined as described in the Assay Protocol. Panel A and B: images of nontreated cells. Panel C and D: treatment with Cytochalasin D.
Functional Studies - Phagocytosis Assay Kit (Red E. coli) (ab235901)
E. coli Standard curve.
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