Phagocytosis Assay Kit (Red Zymosan)
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(5 Publications)
- Ex/Em 540/570 nm
- Validated as macrophage phagocytosis assay.
- includes labeled Zymosan particles and assay buffer. Also includes quenching solution for readout on microscope / plate reader.
- FuncS
Supplier Data
Functional Studies - Phagocytosis Assay Kit (Red Zymosan) (AB234054)
Inhibition of phagocytosis.
J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 h at 37°C prior to addition of 5 μl of Zymosan particles. Phagocytosis was conducted for 2 hours and the amount of engulfed Zymosan was determined as described in the Assay Protocol.
Panel A and B : images of non-treated cells. Panel C and D : treatment with Cytochalasin D.
- FuncS
Unknown
Functional Studies - Phagocytosis Assay Kit (Red Zymosan) (AB234054)
Zymosan Standard curve.
J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 h at 37°C prior to addition of 5 μl of Zymosan particles. Phagocytosis was conducted for 2 hours and the amount of engulfed Zymosan was determined as described in the Assay Protocol.
- FuncS
Supplier Data
Functional Studies - Phagocytosis Assay Kit (Red Zymosan) (AB234054)
Flow cytometry plot.
J774 macrophages were seeded overnight at 5 x 105 of viable cells/well. The next day the cells were pretreated with 20 μM Cytochalasin D for 1 h at 37°C prior to addition of 5 μl of Zymosan particles. Phagocytosis was conducted for 2 hours and the amount of engulfed Zymosan was determined as described in the Assay Protocol.
Black line : untreated control cells; red line : macrophages with engulfed Zymosan particles; blue line : inhibition of phagocytosis by Cytochalasin D.
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Product details
Phagocytosis Assay Kit ab234054 uses Zymosan particles which have been pre-labeled with a red dye.
The assay can be used for the rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. It provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptors ligands (TLR).
The Zymosan particles used in the phagocytosis assay protocol are labeled for fluorescence at Ex/Em 540/570 nm.
Phagocytosis assay protocol summary
- add Red Zymosan reagent to cells, and incubate for 2-3 hours
- harvest cells by centrifugation, remove supernatant, and resuspend cells in Assay Buffer; repeat wash step 3 times
- for fluorescent microscope or fluorometric plate reader analysis, use Quenching Solution
- analyze cells with flow cytometer, fluorescent microscope, or fluorometric plate reader
Related phagocytosis assays
Phagocytosis Assay Kit (Green E.coli) ab235900
Phagocytosis Assay Kit (Red E. coli) ab235901
Phagocytosis Assay Kit (Green Zymosan) ab234053
Phagocytosis Assay Kit (Zymosan, Colorimetric) ab211156
Other notes
This product was previously called K398 Biovision EZCell™ Phagocytosis Assay Kit (Red Zymosan). Biovision was acquired by Abcam in 2021.
In mammals, phagocytosis by phagocytes (e.g., macrophages, dendritic cells, and neutrophils) is essential for a variety of biological events. Phagocytosis comprises a series of events, starting with the binding and recognition of particles by cell surface receptors, followed by the formation of actin-rich membrane extensions around the particle.
Zymosan (Saccharomyces cerevisiae) is prepared from yeast cell wall and consists of protein-carbohydrate complexes. Zymosan is a commonly used pathogen in phagocytosis assays.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Publications (5)
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Nature communications 14:6295 PubMed37813864
2023
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Viruses 14: PubMed36298767
2022
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Frontiers in cell and developmental biology 10:980775 PubMed36158207
2022
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PeerJ 10:e13717 PubMed35818358
2022
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Cell death discovery 7:98 PubMed33972507
2021
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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