Phenylalanine Assay Kit (Colorimetric)
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(1 Review)
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(3 Publications)
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Supplier Data
Functional Studies - Phenylalanine Assay Kit (Colorimetric) (AB241000)
Phenylalanine Standard Curve.
In this instance, standard curve is shown before background subtraction.
- FuncS
Supplier Data
Functional Studies - Phenylalanine Assay Kit (Colorimetric) (AB241000)
Determination in liver lysate and serum : .
Liver tissue from rat and human serum were prepared according to the protocol. For Phe determination, 10 μL liver lysate (1.5 mg/ml) and 80 μL tyrosinase-treated serum were used.
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Product details
Phenylalanine Assay Kit (Colorimetric) (ab241000) provides a quick, simple and accurate method for quantifying Phenylalanine in biological samples. In the assay, Phenylalanine is metabolized with the simultaneous formation of NADH which reacts with a probe to generate absorbance that can be followed colorimetrically at 450 nm. The assay is linear in the range from 0.2 to 10 nmol, allowing accurate determination of concentrations in the range of 1 - 50 μM Phenylalanine.
Other Notes
This product is manufactured by BioVision, an Abcam company and was previously called K481 Phenylalanine Assay Kit (Colorimetric). K481-100 is the same size as the 100 test size of ab241000.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Phenylalanine aids in the formation of proteins by incorporating into the polypeptide chains during ribosomal translation. It is not part of a molecular complex but acts as a free amino acid in various cellular processes. Its conversion to tyrosine by the enzyme phenylalanine hydroxylase is an important step in its metabolism. This conversion is necessary because tyrosine is used in the production of neurotransmitters and hormones.
Pathways
Phenylalanine plays an important role in the phenylalanine and tyrosine catabolic pathway. This pathway is critical for the metabolism of aromatic amino acids eventually leading to the synthesis of important compounds like dopamine and norepinephrine. The enzyme phenylalanine hydroxylase closely related to this pathway shows the connection between phenylalanine and subsequent tyrosine-derived neurotransmitters illustrating its importance in neurochemical regulation.
Product protocols
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Target data
Publications (3)
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MedComm 6:e70100 PubMed39968502
2025
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BMC anesthesiology 23:367 PubMed37946144
2023
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International journal of molecular sciences 21: PubMed33260674
2020
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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