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AB83376

Phenylalanine Assay Kit (Fluorometric)

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(1 Publication)

Phenylalanine Assay Kit ab83376 provides a quick, simple, accurate method for quantifying phenylalanine in biological samples. Readout on any fluorometric (Ex/Em 535/587) plate reader.
3 Images
Functional Studies - Phenylalanine Assay Kit (Fluorometric) (AB83376)
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Lab

Functional Studies - Phenylalanine Assay Kit (Fluorometric) (AB83376)

Phenylalanine fluorimetric standard curve : mean of duplicates (+/- SD) with background readings subtracted

Functional Studies - Phenylalanine Assay Kit (Fluorometric) (AB83376)
  • FuncS

Lab

Functional Studies - Phenylalanine Assay Kit (Fluorometric) (AB83376)

Phenylalanine measured in cell lysates showing quantities (micromoles) per million of cells tested.

Functional Studies - Phenylalanine Assay Kit (Fluorometric) (AB83376)
  • FuncS

Lab

Functional Studies - Phenylalanine Assay Kit (Fluorometric) (AB83376)

Phenylalanine measured in biofluids showing concentration in micromolar.

Key facts

Detection method

Fluorescent

Sample types

Urine, Plasma, Tissue Extracts, Serum, Other biological fluids

Assay type

Quantitative

Sensitivity

> 2 µM

Range

2 - 20 µM

Assay time

60m

Assay Platform

Microplate reader

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Product details

Phenylalanine Assay Kit ab83376 provides a quick, simple, accurate method for quantifying phenylalanine in biological samples.

Phenylalanine assay protocol
In the phenylalanine assay protocol, phenylalanine is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 μM) of Phenylalanine.

Phenylalanine assay protocol summary
- add samples and standards to wells
- add reaction mix and incubate for 60 min
- analyze with a microplate reader

Other Notes
This product was previously called K572 Biovision Phenylalanine Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phenylalanine often abbreviated as Phe is an essential amino acid with a molecular mass of 165.19 g/mol. It serves as a precursor for the biosynthesis of proteins and other compounds. Phenylalanine is transported into cells by a specific membrane transporter and expresses mainly in the liver and brain tissues. In laboratory settings phenylalanine assays and kits are often used to measure its concentration in biological samples which can be colorimetrically or fluorescently assessed.
Biological function summary

Phenylalanine aids in the formation of proteins by incorporating into the polypeptide chains during ribosomal translation. It is not part of a molecular complex but acts as a free amino acid in various cellular processes. Its conversion to tyrosine by the enzyme phenylalanine hydroxylase is an important step in its metabolism. This conversion is necessary because tyrosine is used in the production of neurotransmitters and hormones.

Pathways

Phenylalanine plays an important role in the phenylalanine and tyrosine catabolic pathway. This pathway is critical for the metabolism of aromatic amino acids eventually leading to the synthesis of important compounds like dopamine and norepinephrine. The enzyme phenylalanine hydroxylase closely related to this pathway shows the connection between phenylalanine and subsequent tyrosine-derived neurotransmitters illustrating its importance in neurochemical regulation.

Phenylalanine is strongly associated with the genetic disorder phenylketonuria (PKU) resulting from mutations in the phenylalanine hydroxylase gene. This deficiency leads to high levels of phenylalanine in the blood causing neurotoxicity and cognitive impairments if left untreated. Management of PKU involves a diet low in phenylalanine connecting it to medical nutrition strategies. Furthermore high phenylalanine levels can affect proteins involved in neurotransmitter production linking it to potential neurological complications when not properly regulated.
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Product protocols

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Target data

See full target information Phenylalanine
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Lasers in surgery and medicine 55:490-502 PubMed37051852

2023

Laser-assisted drug delivery of synthetic alpha melanocyte stimulating hormone and L-tyrosine leads to increased pigmentation area and expression of melanogenesis genes in a porcine hypertrophic scar model.

Applications

Unspecified application

Species

Unspecified reactive species

Bonnie C Carney,Mary A Oliver,Sanjana Kurup,Monica Collins,John W Keyloun,Lauren T Moffatt,Jeffrey W Shupp,Taryn E Travis
View all publications
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websiteProtocolBooklet
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Product promise

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