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Phosphate Assay Kit (Colorimetric) (ab65622) provides an easy, quick and simple method for measuring phosphate levels.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Images

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate

Assay type

Enzyme activity

Range

0.001 - 1 mM

Assay time

2h

Sensitivity

> 1 µM

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What's included?

500 Test
Components
Phosphate Reagent
1 x 15 mL
Phosphate Standard
1 x 500 µL

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Phosphate Assay Kit (Colorimetric) (ab65622) provides an easy, quick and simple method for measuring phosphate levels.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate

Assay type

Enzyme activity

Range

0.001 - 1 mM

Assay time

2h

Assay Platform

Microplate reader

Sensitivity

> 1 µM

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Storage information

Please refer to protocols

Notes

Phosphate Assay Kit (Colorimetric) (ab65622) provides an easy, quick and simple method for measuring phosphate levels.

The phosphate assay protocol uses a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around OD = 650nm.

Phosphate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K410 Phosphate Colorimetric Assay Kit. K410-500 is the same size as the 500 test size of ab65622.

This assay can be used with biological fluids but also inorganic samples such as algal blooms and water from run-off areas of high fertilizer use.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622), expandable thumbnail
    Larissa S.Moniz, et al., Scientific Reports volume 7, Fig 5, Article number: 39985 (2017)

    Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622)

    Immunoprecipitates of Flag-NT5C ectopically expressed in HEK293 cells were incubated with 5 mM of the indicated nucleotides. Phosphate release was measured using a malachite green colorimetric assay (ab65622) and expressed as a percent of total nucleotide. The experiment was performed in duplicate and repeated 3 times independently. Error bars are sem.

  • Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622), expandable thumbnail

    Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622), expandable thumbnail

    Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622)

    Phosphate measured in tissue lysates showing quantity (μmol) per mg of extracted protein.

    Protein concentration for samples varied from 9 mg/mL to 14 mg/mL. Samples were diluted 400-4000 fold.

  • Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622), expandable thumbnail

    Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622)

    Phosphate measured in cell culture lysates showing quantity (μmol) per mg of extracted protein.

    Samples with the concentration of 1.3e7 cells/mL were used. Samples were diluted 400-4000 fold.

  • Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622), expandable thumbnail

    Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622)

    Phosphate measured in biological fluids showing quantity (μmol) per mL of tested sample. Samples were diluted 100-1000 fold.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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