Phosphate Assay Kit (Colorimetric)

• FuncS

Lab

Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622)

Phosphate measured in biological fluids showing quantity (μmol) per mL of tested sample. Samples were diluted 100-1000 fold.

• FuncS

Lab

Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622)

Phosphate measured in tissue lysates showing quantity (μmol) per mg of extracted protein.

Protein concentration for samples varied from 9 mg/mL to 14 mg/mL. Samples were diluted 400-4000 fold.

• FuncS

Lab

Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

• FuncS

Lab

Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622)

Phosphate measured in cell culture lysates showing quantity (μmol) per mg of extracted protein.

Samples with the concentration of 1.3e7 cells/mL were used. Samples were diluted 400-4000 fold.

• FuncS

Unknown

Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622)

Immunoprecipitates of Flag-NT5C ectopically expressed in HEK293 cells were incubated with 5 mM of the indicated nucleotides. Phosphate release was measured using a malachite green colorimetric assay (ab65622) and expressed as a percent of total nucleotide. The experiment was performed in duplicate and repeated 3 times independently. Error bars are sem.

Larissa S.Moniz, et al., Scientific Reports volume 7, Fig 5, Article number: 39985 (2017)

• Other

Supplier Data

Other - Phosphate Assay Kit (Colorimetric) (AB65622)

Representative image of Phosphate Assay Kit (Colorimetric) ab65622

Components shown from left to right :

- Phosphate Standard

- Phosphate Reagent

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.