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Phosphate Assay Kit (Colorimetric) (ab65622) provides an easy, quick and simple method to quantify phosphate levels. Readout on any colorimetric (650nm) plate reader.

- Cited in >90 publications
-Complete kit including standard curve for quantitation
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Images

Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622), expandable thumbnail
  • Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622), expandable thumbnail
  • Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622), expandable thumbnail
  • Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622), expandable thumbnail
  • Functional Studies - Phosphate Assay Kit (Colorimetric) (AB65622), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
Assay type
Enzyme activity
Range
0.001 - 1 mM
Assay time
2h
Sensitivity
> 1 µM

Associated Products

Select an associated product type

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What's included?

500 Test
Components
Phosphate Reagent
1 x 15 mL
Phosphate Standard
1 x 0.5 mL

Recommended products

Phosphate Assay Kit (Colorimetric) (ab65622) provides an easy, quick and simple method to quantify phosphate levels. Readout on any colorimetric (650nm) plate reader.

- Cited in >90 publications
-Complete kit including standard curve for quantitation
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method
Colorimetric
Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
Assay type
Enzyme activity
Range
0.001 - 1 mM
Assay time
2h
Assay Platform
Microplate reader
Sensitivity
> 1 µM

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient

Notes

Phosphate Assay Kit (Colorimetric) (ab65622) provides an easy, quick and simple method for measuring phosphate levels.

The phosphate assay protocol uses a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around OD = 650nm.

Phosphate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K410 Phosphate Colorimetric Assay Kit. K410-500 is the same size as the 500 test size of ab65622.

This assay can be used with biological fluids but also inorganic samples such as algal blooms and water from run-off areas of high fertilizer use.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Phosphate a simple inorganic chemical functions mechanically as a component of various essential biological molecules such as nucleic acids ATP and phospholipids. Phosphate ions serve as an important player in cellular energy transfer through involvement in phosphorylation reactions. It's present abundantly in living organisms especially in the form of calcium phosphate in bones and teeth. The molecular weight of a phosphate ion is approximately 94.97 g/mol. Expressed ubiquitously across different tissues and cells phosphate is also available in multiple assay formats like the phosphate assay kit and phosphorus assay kits for scientific research.

Biological function summary

Phosphate plays a role in numerous cellular processes beyond its participation in energy metabolism. It contributes critically to cellular signaling pathways as a part of second messengers like cyclic AMP and is a core part of protein kinase and phosphatase complexes which regulate many cellular functions. Moreover phosphate acts in buffering systems maintaining cellular pH balance. In the cellular context the probe and colorimetric malachite green assay often quantify phosphate levels aiding in understanding phosphate homeostasis in biochemical studies.

Pathways

The chemical phosphate is integral to significant pathways such as glycolysis and the citric acid cycle where it assists in energy production and storage. It associates with proteins like hexokinase in the glycolytic pathway and is critical for the formation of high-energy the phosphate bonds in ATP. As phosphate cycles through these pathways it continuously modulates various metabolic activities and biochemical reactions within the cell highlighting its universal importance.

Associated diseases and disorders

Phosphate imbalances relate to conditions such as chronic kidney disease and hypophosphatemia. In chronic kidney disease altered phosphate homeostasis can lead to cardiovascular complications due to phosphate's interactions with proteins such as fibroblast growth factor 23 (FGF23) which regulates phosphate metabolism. In hypophosphatemia a deficiency in phosphate can result in skeletal disorders highlighting the need for precise phosphate detection and monitoring in clinical settings.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622), expandable thumbnail

    Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622)

    Phosphate measured in biological fluids showing quantity (μmol) per mL of tested sample. Samples were diluted 100-1000 fold.

  • Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622), expandable thumbnail
    Larissa S.Moniz, et al., Scientific Reports volume 7, Fig 5, Article number: 39985 (2017)

    Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622)

    Immunoprecipitates of Flag-NT5C ectopically expressed in HEK293 cells were incubated with 5 mM of the indicated nucleotides. Phosphate release was measured using a malachite green colorimetric assay (ab65622) and expressed as a percent of total nucleotide. The experiment was performed in duplicate and repeated 3 times independently. Error bars are sem.

  • Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622), expandable thumbnail

    Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622), expandable thumbnail

    Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622)

    Phosphate measured in tissue lysates showing quantity (μmol) per mg of extracted protein.

    Protein concentration for samples varied from 9 mg/mL to 14 mg/mL. Samples were diluted 400-4000 fold.

  • Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622), expandable thumbnail

    Functional Studies - Phosphate Assay Kit (Colorimetric) (ab65622)

    Phosphate measured in cell culture lysates showing quantity (μmol) per mg of extracted protein.

    Samples with the concentration of 1.3e7 cells/mL were used. Samples were diluted 400-4000 fold.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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