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AB273335

Phosphatidic Acid Assay Kit (Fluorometric)

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(1 Publication)

Phosphatidic Acid Assay Kit (Fluorometric) (ab273335) is a plate-based enzymatic assay for quantitation of Phosphatidic Acid (PA) in cells and tissues.
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Functional Studies - Phosphatidic Acid Assay Kit (Fluorometric) (AB273335)
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Supplier Data

Functional Studies - Phosphatidic Acid Assay Kit (Fluorometric) (AB273335)

Example data.

Assay Specificity : Equimolar amounts (20 nmol) of phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylinositol (PI) and cardiolipin (CL) show interference less than 20% with respect of Phosphatidic Acid. Assay shows specificity for phosphatidic acid.

Functional Studies - Phosphatidic Acid Assay Kit (Fluorometric) (AB273335)
  • FuncS

Supplier Data

Functional Studies - Phosphatidic Acid Assay Kit (Fluorometric) (AB273335)

Example data.

Phosphatidic acid standard curve.

Functional Studies - Phosphatidic Acid Assay Kit (Fluorometric) (AB273335)
  • FuncS

Supplier Data

Functional Studies - Phosphatidic Acid Assay Kit (Fluorometric) (AB273335)

Example data.

Phosphatidic acid content in rat liver and HEK 293 cells. For rat liver extract, 100 mg tissue was homogenized in 1 ml PA assay buffer. Lipid extraction was carried out and was re-suspended in 200 μl 5% Triton X-100 solution. For HEK293, cells were grown to confluency and harvested using a rubber policeman.

Key facts

Detection method

Fluorescent

Sample types

Cell culture extracts, Tissue Extracts

Results type

Quantitative

Assay Platform

Microplate

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Product details

Phosphatidic Acid Assay Kit (Fluorometric) (ab273335) is a plate-based enzymatic assay for quantitation of Phosphatidic Acid (PA) in cells and tissues.
The PA Converter hydrolyzes PA to form an intermediate which, in the presence of a developer and enzyme mix, converts a non-fluorescent probe to a fluorescent product (Ex/Em= 535/587 nm) that can be quantified. The Converter is specific to PA and does not hydrolyze other phospholipids (e.g. those with more complex head groups such as phosphatidylcholine or cardiolipin), allowing direct quantitation of PA.
This kit can detect as low as 40 pmol PA per well.

This product is manufactured by BioVision, an Abcam company and was previously called K748 PicoProbeTM Phosphatidic Acid Assay Kit (Fluorometric). K748-100 is the same size as the 100 test size of ab273335.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphatidic acid known also as PA is a lipid molecule involved in cellular signaling. It has a molecular mass of about 478 g/mol. This molecule is found widely within cell membranes serving as a minor but essential phospholipid component. PA performs a mechanical role acting as a precursor for biosynthesis of other lipids. It is also important for protein binding at membranes influencing various signaling pathways within the cell. The expression of phosphatidic acid is variable across different cell types and tissues reflecting its multiple roles in cell function.
Biological function summary

PA is important in membrane curvature and vesicle trafficking. It contributes to the balance between membrane bending and tension forces in cells. PA often exists in a complex with proteins such as diacylglycerol kinase and phospholipase D which regulate its levels. These interactions illustrate its dynamic role in cell signaling and structural functions. It plays key roles in mitogenesis and muscle regeneration emphasizing its impact on cellular growth and repair processes.

Pathways

PA participates significantly in the mTOR signaling pathway and the Ras/Raf/MEK/ERK pathway. Through these pathways it connects with proteins like mTOR and Ras influencing cell metabolism growth and survival. In the mTOR pathway PA regulates nutrient-sensing and energy metabolism. It is vital to cellular responses to growth factors and stress signals coordinating with Ras proteins to manage cell proliferation and apoptosis.

PA is linked to cancer and metabolic syndrome. Its role in mTOR signaling has implications for tumor development and progression making it a target for anticancer therapies. Excessive PA can disrupt metabolic pathways potentially leading to insulin resistance and type 2 diabetes. PA interacts with proteins such as mTOR and reactive oxygen species in these conditions highlighting its impact on disease development. The understanding of PA in these contexts could contribute to targeted therapeutic strategies.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 4:100880 PubMed36603576

2023

DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunkoo Kang,Haksoo Lee,Kyeongmin Kim,Eunguk Shin,Byeongsoo Kim,JiHoon Kang,Bohkyung Kim,Jung Sub Lee,Jae-Myung Lee,HyeSook Youn,BuHyun Youn
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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