Phosphatidylglycerol Assay Kit (Fluorometric)
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(1 Publication)
- FuncS
Supplier Data
Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)
PG standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
- FuncS
Supplier Data
Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)
Specificity of assay, when used as described, for Phosphatidylglycerol (PG) versus Phosphatidylethanolamine (PE), Phosphatidic Acid (PA), Phosphatidylinositol (PI), and cardiolipin (CL). The amount of phospholipid for each sample was 1 nmole.
- FuncS
Supplier Data
Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)
Determination of Phosphatidylglycerol in lipid extracted from lung tissue
- FuncS
Supplier Data
Functional Studies - Phosphatidylglycerol Assay Kit (Fluorometric) (AB273288)
Determination of total PG concentration in lipid extracted from brain lysate
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Product details
Phosphatidylglycerol Assay Kit (Fluorometric) (ab273288) utilizes specific enzymes to generate an intermediate that reacts with a probe. This yields a signal that can be quantified fluorometrically and is proportional to the amount of Phosphatidylglycerol (PG) present in the sample, unaffected by the presence of phosphatidic acid, cardiolipin, or other lipids. When used as described, the assay is highly selective against other phospholipids and capable of detecting as little as 20 pmoles of phosphatidylglycerol.
This product is manufactured by BioVision, an Abcam company and was previously called K488 Phosphatidylglycerol Assay Kit (Fluorometric). K488-100 is the same size as the 100 test size of ab273288.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This lipid is a building block of membranes and acts in signaling processes. While PG does not form part of a larger protein complex it interacts closely with other lipids and proteins to maintain membrane integrity and function. The presence of Phosphatidylglycerol in pulmonary surfactant exemplifies its role in maintaining lung function and preventing alveolar collapse during exhalation. In specific bacteria PG also contributes to energy metabolism by participating in the synthesis of other phospholipids.
Pathways
Phosphatidylglycerol contributes to lipid metabolic processes and is part of the CDP-diacylglycerol pathway. This pathway is important for synthesizing other phospholipids such as cardiolipin which plays an important role in maintaining mitochondrial function. As PG interacts with proteins and enzymes related to these pathways such as phospholipase D and phosphatidylglycerophosphate synthase it influences membrane dynamics and cellular signaling.
Product protocols
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Publications (1)
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Cell metabolism 34:1960-1976.e9 PubMed36476935
2022
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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