Phosphoenolpyruvic acid (PEP) Assay Kit
Be the first to review this product! Submit a review
|
(2 Publications)
- FuncS
Supplier Data
Functional Studies - Phosphoenolpyruvic acid (PEP) Assay Kit (AB204713)
Typical PEP Standard calibration curve using colorimetric reading.
- FuncS
Supplier Data
Functional Studies - Phosphoenolpyruvic acid (PEP) Assay Kit (AB204713)
Typical PEP Standard calibration curve using fluorometric reading.
Complementary Products
Product details
Phosphoenolpyruvic acid (PEP) Assay Kit (ab204713) provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples. In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (λmax = 570 nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, sensitive and reliable. The detection limit is approximately 1 µM PEP in biological samples.
This product is manufactured by BioVision, an Abcam company and was previously called K365 PEP Colorimetric/Fluorometric Assay Kit. K365-100 is the same size as the 100 test size of ab204713.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of phosphoenolpyruvate extends beyond glycolysis. It acts as a significant player in gluconeogenesis aiding in the conversion of lactate and other substrates into glucose. PEP is an important precursor for several biosynthetic pathways including amino acid synthesis. It is not part of a molecular complex but interacts with several enzymes such as pyruvate kinase that facilitate its conversion into pyruvate releasing energy stored in its phosphate bond.
Pathways
Phosphoenolpyruvate is central to both glycolysis and gluconeogenesis. It stands at a critical juncture in the glycolytic pathway where the PEP to pyruvate conversion catalyzed by pyruvate kinase is a major step for ATP synthesis. In gluconeogenesis PEP converts back to glucose ensuring energy balance during fasting states. PEP is closely related to proteins such as pyruvate kinase and enolase which are key players in the glycolytic and gluconeogenic pathways respectively.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
Journal of the European Academy of Dermatology and Venereology : JEADV 37:2067-2079 PubMed37247195
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cancer & metabolism 7:8 PubMed31388420
2019
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com