Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric) ab155898 is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.
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- Nutrients 15:2023Effect of Moderate-Intense Training and Detraining on Glucose Metabolism, Lipid Profile, and Liver Enzymes in Male Wistar Rats: A Preclinical Randomized Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHira Shakoor,Jaleel Kizhakkayil,Mariyam Khalid,Amar Mahgoub,Carine PlatatPubMed 37686852
- Cancer research 83:3478-34912023The Ratio of Key Metabolic Transcripts Is a Predictive Biomarker of Breast Cancer Metastasis to the Lung.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepti Mathur,Chen Liao,Wendy Lin,Alessandro La Ferlita,Salvatore Alaimo,Samuel Taylor,Yi Zhong,Christine Iacobuzio-Donahue,Alfredo Ferro,Joao B XavierPubMed 37526524
- Antioxidants (Basel, Switzerland) 12:2022Specificity Protein 1-Mediated Promotion of CXCL12 Advances Endothelial Cell Metabolism and Proliferation in Pulmonary Hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvan R DeVallance,Christopher M Dustin,Daniel Simoes de Jesus,Imad Al Ghouleh,John C Sembrat,Eugenia Cifuentes-Pagano,Patrick J PaganoPubMed 36670936
- Stem cell reviews and reports 19:767-7832022Maternal Undernutrition Induces Cell Signalling and Metabolic Dysfunction in Undifferentiated Mouse Embryonic Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPooja Khurana,Andrew Cox,Barira Islam,Judith J Eckert,Sandrine Willaime-Morawek,Joanna M Gould,Neil R Smyth,Patrick C McHugh,Tom P FlemingPubMed 36517693
- Nature communications 13:67542022Altered glycolysis triggers impaired mitochondrial metabolism and mTORC1 activation in diabetic β-cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth Haythorne,Matthew Lloyd,John Walsby-Tickle,Andrei I Tarasov,Jonas Sandbrink,Idoia Portillo,Raul Terron Exposito,Gregor Sachse,Malgorzata Cyranka,Maria Rohm,Patrik Rorsman,James McCullagh,Frances M AshcroftPubMed 36376280
- Cell reports 41:1115982022MsrB1-regulated GAPDH oxidation plays programmatic roles in shaping metabolic and inflammatory signatures during macrophage activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Jung Yoo,Dong Wook Choi,Yeon Jin Roh,Yoon-Mi Lee,Ji-Hong Lim,Soohak Eo,Ho-Jae Lee,Na Young Kim,Seohyun Kim,Sumin Cho,Gyumin Im,Byung Cheon Lee,Ji Hyung KimPubMed 36351405
- Nutrients 14:2022Partial Substitution of Glucose with Xylitol Prolongs Survival and Suppresses Cell Proliferation and Glycolysis of Mice Bearing Orthotopic Xenograft of Oral Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuraporn Sahasakul,Wannee Angkhasirisap,Aroonwan Lam-Ubol,Amornrat Aursalung,Daisuke Sano,Kentaro Takada,Dunyaporn TrachoothamPubMed 35631164
- Nature communications 13:23242022Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Ho Koh,Mark W Pataky,Surendra Dasari,Katherine A Klaus,Ivan Vuckovic,Gregory N Ruegsegger,Arathi Prabha Kumar,Matthew M Robinson,K Sreekumaran NairPubMed 35484130
- IUBMB life 72:2481-24982020Glycolytic and immunological alterations in human U937 monocytes in response to H1N1 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTarek Kamal Motawi,Nancy Nabil Shahin,Kareem Awad,Amany Sayed Maghraby,Dina Nadeem Abd-Elshafy,Mahmoud Mohamed BahgatPubMed 32941696
- Cell metabolism 32:420-436.e122020Sirt2 Inhibition Enhances Metabolic Fitness and Effector Functions of Tumor-Reactive T Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesImene Hamaidi,Lin Zhang,Nayoung Kim,Min-Hsuan Wang,Cristina Iclozan,Bin Fang,Min Liu,John M Koomen,Anders E Berglund,Sean J Yoder,Jiqiang Yao,Robert W Engelman,Ben C Creelan,Jose R Conejo-Garcia,Scott J Antonia,James J Mulé,Sungjune KimPubMed 32768387
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Range
- 2 - 10 nmol/well
- Assay time
- 1h 30m
- Sensitivity
- < 1 mU/well
Target data
Function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity).
Additional Targets
PFKM, PFKP
Alternative names
ATP-PFK, PFK-L, 6-phosphofructokinase type B, Phosphofructo-1-kinase isozyme B, Phosphohexokinase, PFK-B, PFKL
What's included?
Recommended products
Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric) ab155898 is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Range
- 2 - 10 nmol/well
- Assay time
- 1h 30m
- Assay Platform
- Microplate reader
- Sensitivity
- < 1 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phosphofructokinase Activity Assay kit ab155898 is a mix-and-read assay to quantify 6-PFK activity. Readout on any colorimetric (450 nm) plate reader.
PFK activity assay principle
In the PFK activity assay protocol, PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.
PFK activity assay protocol summary
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader over 20-60 min
Other notes
This product was previously called K776 Biovision Phosphofructokinase (PFK) Activity Colorimetric Assay Kit and 6-Phosphofructokinase Activity Assay Kit (Colorimetric). Biovision was acquires by Abcam in 2021.
Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet).
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
6-Phosphofructokinase often abbreviated as PFK or PFK-1 is an important enzyme in the glycolytic pathway catalyzing the phosphorylation of fructose-6-phosphate to fructose-16-bisphosphate. This reaction is a major rate-limiting step in glycolysis controlling the pace of glucose breakdown for energy production. PFK-1 is a tetrameric enzyme with a molecular mass of about 340 kDa. It is expressed in a variety of tissues including muscle liver and brain reflecting its essential role in energy metabolism across different tissues.
- Biological function summary
This enzyme plays an important role in regulating glycolysis where it ensures efficient energy production under varying cell conditions. PFK-1 acts as a metabolic sensor responding to cellular energy demands by allosteric regulation through ATP ADP AMP and citrate. By forming part of a multisubunit complex it regulates substrate flux within glycolysis and changes in PFK-1 activity directly affect how cells respond to energy needs and stress.
- Pathways
6-phosphofructokinase functions critically within glycolysis and by extension influences the TCA cycle. The activity of this enzyme links to key metabolites like ATP and AMP which regulate its function. Furthermore PFK-1 activity relates to the activity of enzymes such as hexokinase and pyruvate kinase forming a coordinated system that controls the rate of glucose oxidation and energy yield highlighting its pivotal role in cellular metabolic networks.
- Associated diseases and disorders
PFK-1 has connections to conditions such as cancer and glycogen storage disease type VII also known as Tarui's disease. In cancer enhanced glycolytic flux often accompanies increased PFK-1 activity supporting tumour growth. In Tarui's disease mutations in the PFKM gene lead to deficient PFK-1 activity in muscle causing impaired glycogen metabolism. This enzyme's dysregulation can lead to significant metabolic disturbances making it a target for therapeutic interventions in such conditions.
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Functional Studies - Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric) (ab155898)
PFK activity determined in bovine liver and HeLa cell lysate. Assay performed following kit protocol.
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