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AB155898

Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric)

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(31 Publications)

Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric) ab155898 is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.

View Alternative Names

ATP-PFK, PFK-L, 6-phosphofructokinase type B, Phosphofructo-1-kinase isozyme B, Phosphohexokinase, PFK-B, PFKL

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Functional Studies - Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric) (AB155898)
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Supplier Data

Functional Studies - Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric) (AB155898)

PFK activity determined in bovine liver and HeLa cell lysate. Assay performed following kit protocol.

Schematic Diagram - Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric) (AB155898)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric) (AB155898)

Representative image of 6-Phosphofructokinase Activity Assay Kit (Colorimetric) ab155898

Components shown from left to right :

- NADH Standard I

- PFK Substrate

- Developer Solution III

- PFK Positive Control

- ATP III

- CK Enzyme Mix

- Assay Buffer 7

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cell Lysate

Assay type

Enzyme activity

Sensitivity

< 1 mU/well

Range

2 - 10 nmol/well

Assay time

1h 30m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phosphofructokinase Activity Assay kit ab155898 is a mix-and-read assay to quantify 6-PFK activity. Readout on any colorimetric (450 nm) plate reader.

PFK activity assay principle
In the PFK activity assay protocol, PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.

PFK activity assay protocol summary
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader over 20-60 min

Other notes
This product was previously called K776 Biovision Phosphofructokinase (PFK) Activity Colorimetric Assay Kit and 6-Phosphofructokinase Activity Assay Kit (Colorimetric). Biovision was acquires by Abcam in 2021.

Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet).

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab155898-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"PFK Substrate", "number":"AB155898-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PFK Positive Control", "number":"AB155898-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"CK Enzyme Mix", "number":"AB155898-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 7", "number":"AB155898-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADH Standard I", "number":"AB155898-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB155898-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ATP III", "number":"AB155898-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

6-Phosphofructokinase often abbreviated as PFK or PFK-1 is an important enzyme in the glycolytic pathway catalyzing the phosphorylation of fructose-6-phosphate to fructose-16-bisphosphate. This reaction is a major rate-limiting step in glycolysis controlling the pace of glucose breakdown for energy production. PFK-1 is a tetrameric enzyme with a molecular mass of about 340 kDa. It is expressed in a variety of tissues including muscle liver and brain reflecting its essential role in energy metabolism across different tissues.
Biological function summary

This enzyme plays an important role in regulating glycolysis where it ensures efficient energy production under varying cell conditions. PFK-1 acts as a metabolic sensor responding to cellular energy demands by allosteric regulation through ATP ADP AMP and citrate. By forming part of a multisubunit complex it regulates substrate flux within glycolysis and changes in PFK-1 activity directly affect how cells respond to energy needs and stress.

Pathways

6-phosphofructokinase functions critically within glycolysis and by extension influences the TCA cycle. The activity of this enzyme links to key metabolites like ATP and AMP which regulate its function. Furthermore PFK-1 activity relates to the activity of enzymes such as hexokinase and pyruvate kinase forming a coordinated system that controls the rate of glucose oxidation and energy yield highlighting its pivotal role in cellular metabolic networks.

PFK-1 has connections to conditions such as cancer and glycogen storage disease type VII also known as Tarui's disease. In cancer enhanced glycolytic flux often accompanies increased PFK-1 activity supporting tumour growth. In Tarui's disease mutations in the PFKM gene lead to deficient PFK-1 activity in muscle causing impaired glycogen metabolism. This enzyme's dysregulation can lead to significant metabolic disturbances making it a target for therapeutic interventions in such conditions.
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Product protocols

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Target data

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed : 22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity).
See full target information PFKL

Additional targets

PFKM,PFKP
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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40243606

2025

High Glucose Reduces Influenza and Parainfluenza Virus Productivity by Altering Glycolytic Pattern in A549 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kareem Awad,Maha Abdelhadi,Ahmed M Awad

Frontiers in cell and developmental biology 13:1529093 PubMed39944083

2025

neural stem progenitor cells exhibit a transient metabolic shift toward glycolysis during spinal cord regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Paula G Slater,Miguel E Domínguez-Romero,Guillermo Campos,Vania Aravena,Javier Cavieres-Lepe,Verónica Eisner

NPJ precision oncology 8:252 PubMed39501021

2024

UBR5 metabolically reprograms nasopharyngeal carcinoma cells to promote glycolysis and M2 polarization via SPLUNC1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Huai Liu,Yanxian Li,Ling Tang,Xiaowen Sun,Wenji Xie,Tengfei Xiao,Wangning Gu,Hongmin Yang,Hui Wang,Pan Chen

Biomedicines 12: PubMed39062148

2024

High Glucose Increases Lactate and Induces the Transforming Growth Factor Beta-Smad 1/5 Atherogenic Pathway in Primary Human Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Kareem Awad,Laura Kakkola,Ilkka Julkunen

Nature metabolism 6:494-513 PubMed38443593

2024

Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of inflammatory pain.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastián Marty-Lombardi,Shiying Lu,Wojciech Ambroziak,Katrin Schrenk-Siemens,Jialin Wang,Anna A DePaoli-Roach,Anna M Hagenston,Hagen Wende,Anke Tappe-Theodor,Manuela Simonetti,Hilmar Bading,Jürgen G Okun,Rohini Kuner,Thomas Fleming,Jan Siemens

Nutrients 15: PubMed37686852

2023

Effect of Moderate-Intense Training and Detraining on Glucose Metabolism, Lipid Profile, and Liver Enzymes in Male Wistar Rats: A Preclinical Randomized Study.

Applications

Unspecified application

Species

Unspecified reactive species

Hira Shakoor,Jaleel Kizhakkayil,Mariyam Khalid,Amar Mahgoub,Carine Platat

Cancer research 83:3478-3491 PubMed37526524

2023

The Ratio of Key Metabolic Transcripts Is a Predictive Biomarker of Breast Cancer Metastasis to the Lung.

Applications

Unspecified application

Species

Unspecified reactive species

Deepti Mathur,Chen Liao,Wendy Lin,Alessandro La Ferlita,Salvatore Alaimo,Samuel Taylor,Yi Zhong,Christine Iacobuzio-Donahue,Alfredo Ferro,Joao B Xavier

Glycobiology 33:626-636 PubMed37364046

2023

Role of the ST6GAL1 sialyltransferase in regulating ovarian cancer cell metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Robert B Jones,Austin D Silva,Katherine E Ankenbauer,Colleen M Britain,Asmi Chakraborty,Jamelle A Brown,Scott W Ballinger,Susan L Bellis

Antioxidants (Basel, Switzerland) 12: PubMed36670936

2022

Specificity Protein 1-Mediated Promotion of CXCL12 Advances Endothelial Cell Metabolism and Proliferation in Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Evan R DeVallance,Christopher M Dustin,Daniel Simoes de Jesus,Imad Al Ghouleh,John C Sembrat,Eugenia Cifuentes-Pagano,Patrick J Pagano

Stem cell reviews and reports 19:767-783 PubMed36517693

2022

Maternal Undernutrition Induces Cell Signalling and Metabolic Dysfunction in Undifferentiated Mouse Embryonic Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pooja Khurana,Andrew Cox,Barira Islam,Judith J Eckert,Sandrine Willaime-Morawek,Joanna M Gould,Neil R Smyth,Patrick C McHugh,Tom P Fleming
View all publications
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