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AB211104

Phosphoglucomutase Activity Assay Kit (Fluorometric)

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(1 Publication)

Phosphoglucomutase Activity Assay Kit (Fluorometric) (ab211104) provides a sensitive, simple and convenient method for measuring Phosphoglucomutase (PGM) activity in cell and tissue lysates.

View Alternative Names

Phosphoglucomutase-1, PGM 1, Glucose phosphomutase 1, PGM1

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Functional Studies - Phosphoglucomutase Activity Assay Kit (Fluorometric) (AB211104)
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Functional Studies - Phosphoglucomutase Activity Assay Kit (Fluorometric) (AB211104)

Phosphoglucomutase activity.

PGM Activity Assay Kit (ab211104) Kinetic curves showing PGM activity in positive control (PosCtrl, included in kit), Jurkat cell lysate (0.4 μg) and rat liver homogenate (0.2 μg). Curves of background control wells are also shown.

Functional Studies - Phosphoglucomutase Activity Assay Kit (Fluorometric) (AB211104)
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Supplier Data

Functional Studies - Phosphoglucomutase Activity Assay Kit (Fluorometric) (AB211104)
Functional Studies - Phosphoglucomutase Activity Assay Kit (Fluorometric) (AB211104)
  • FuncS

Supplier Data

Functional Studies - Phosphoglucomutase Activity Assay Kit (Fluorometric) (AB211104)

Example Glucose-6-phosphate standard calibration curve. Actual values will vary by plate reader.

Functional Studies - Phosphoglucomutase Activity Assay Kit (Fluorometric) (AB211104)
  • FuncS

Supplier Data

Functional Studies - Phosphoglucomutase Activity Assay Kit (Fluorometric) (AB211104)

PGM Activity Assay Kit (ab211104) Typical Phosphoglucomutase specific activity in Jurkat cell lysate (0.4 μg) and rat liver homogenate (0.2 μg).

Key facts

Detection method

Fluorescent

Sample types

Tissue Lysate, Cell Lysate

Reacts with

Mammals

Assay type

Enzyme activity

Results type

Quantitative

Sensitivity

<= 1 µU

Assay Platform

Microplate reader

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Primary Antibodies

AB52488

Anti-Lactate Dehydrogenase antibody [EP1566Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [EP1566Y] (AB52488)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phosphoglucomutase Activity Assay Kit (Fluorometric) (ab211104) provides a sensitive, simple and convenient method for measuring Phosphoglucomutase (PGM) activity in cell and tissue lysates. The assay is based on the ability of Phosphoglucomutase to convert glucose-1-phosphate to glucose-6-phosphate. Glucose-6-phosphate is then oxidized by Glucose-6-phosphate dehydrogenase (G6PDH) to form NADPH, which in turn reduces the probe to generate an intense fluorescence product that can detected at Ex/Em = 535/587 nm.

This assay can detect as low as 20 µU of Phosphoglucomutase activity.

This product is manufactured by BioVision, an Abcam company and was previously called K770 PicoProbe™ Phosphoglucomutase Assay Kit (Fluorometric). K770-100 is the same size as the 100 test size of ab211104.

Phosphoglucomutase (PGM, EC 5.4.2.2) facilitates the interconversion of glucose-1-phosphate (G1P) and glucose-6-phosphate (G6P). Glycogen breakdown generates G1P, which is converted to G6P by PGM. G6P can then go into the glycolytic pathway to generate ATP, or to the pentose phosphate pathway to generate ribose and NADPH. On the other hand, when cells have extra energy, G6P is converted by PGM into G1P to generate glycogen.

In humans, two isoforms have been described, PGM I and PGM II. PGM deficiency leads to glucose storage disease.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab211104-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"PGM Substrate", "number":"AB211104-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix G", "number":"AB211104-CMP03", "productcode":"" }, { "size":"1 x 400 µL", "name":"PicoProbe I", "number":"AB211104-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PGM Positive Control", "number":"AB211104-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"G6P Standard", "number":"AB211104-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix P", "number":"AB211104-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB211104-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aciculin also known as gamma-filamin or filamin C is a protein involved in mechanotransduction and cellular structural integrity. It has a molecular mass of approximately 290 kDa. Aciculin is expressed primarily in skeletal and cardiac muscle tissues providing stability and flexibility to the cellular cytoskeleton. It plays an essential role in connecting the actin cytoskeleton to various cell membrane components facilitating cellular shape and movement.
Biological function summary

Aciculin is essential for cytoskeletal organization and cell adhesion processes. It acts within a complex network of proteins that includes integrins and sarcoglycans assisting in mechanical signal transduction across muscle cells. This interaction supports muscle cell structure and integrity allowing proper muscle function during contraction and relaxation phases. It also helps in repairing cell membrane disruptions caused by mechanical stress.

Pathways

The protein contributes significantly to actin filament organization and integrin-mediated signaling pathways. These pathways are important for maintaining muscle cell strength and stability. Aciculin interacts with proteins like vinculin and talin enhancing its role in actin cytoskeleton rearrangement processes and integrin signal transduction. As a result it helps ensure cell adhesion and motility by linking cytoskeletal elements to extracellular matrix components.

Mutations or defects in Aciculin are associated with conditions such as myofibrillar myopathy and cardiomyopathy. These disorders are characterized by muscle weakness and deterioration due to impaired cytoskeletal stability. In these cases Aciculin’s interaction with dystrophin becomes critical as dystrophin abnormalities can compound issues with muscle integrity and function. Understanding how Aciculin functions in these contexts is important for developing therapeutic approaches to muscular disorders.
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Product protocols

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Target data

Catalyzes the reversible isomerization of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate (PubMed : 15378030, PubMed : 25288802). The mechanism proceeds via the intermediate compound alpha-D-glucose 1,6-bisphosphate (Probable) (PubMed : 25288802). This enzyme participates in both the breakdown and synthesis of glucose (PubMed : 17924679, PubMed : 25288802).
See full target information PGM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 10: PubMed33287293

2020

Structure and Characterization of Phosphoglucomutase 5 from Atlantic and Baltic Herring-An Inactive Enzyme with Intact Substrate Binding.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Gustafsson,Ulrich Eckhard,Weihua Ye,Erik D Enbody,Mats Pettersson,Per Jemth,Leif Andersson,Maria Selmer
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websiteProtocolBooklet
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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