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AB273328

Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric)

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(4 Publications)

Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (ab273328) provides a quick and easy method for monitoring PHGDH activity in a variety of samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

PGDH3, PHGDH, D-3-phosphoglycerate dehydrogenase, 3-PGDH, 2-oxoglutarate reductase, Malate dehydrogenase

3 Images
Functional Studies - Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (AB273328)
  • FuncS

Supplier Data

Functional Studies - Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (AB273328)

Example data.

Kinetic measurement of Phosphoglycerate Dehydrogenase activity from various samples.

Functional Studies - Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (AB273328)
  • FuncS

Supplier Data

Functional Studies - Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (AB273328)

Example data.

Relative PHGDH Activity was calculated in lysates prepared from HEK cells (4.5μg) and HeLa cells (7.8μg).

Functional Studies - Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (AB273328)
  • FuncS

Supplier Data

Functional Studies - Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (AB273328)

Example data.

NADH Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Enzyme activity

Results type

Quantitative

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric) (ab273328) provides a quick and easy method for monitoring PHGDH activity in a variety of samples. In this kit, Phosphoglycerate Dehydrogenase converts 3-phosphoglycerate and NAD into 3-phosphohydroxyglerate and NADH respectively. The oxidation of NADH reduces a probe generating a strong, stable absorbance signal (OD: 450 nm).

The assay is simple, sensitive, high-throughput adaptable and can detect Phosphoglycerate Dehydrogenase activity less than 0.1 mU per sample.

Other Notes
This product was previously called K569 Biovision Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273328-100Test", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"PHGDH Positive Control", "number":"AB273328-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"PHGDH Assay Buffer", "number":"AB273328-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADH Standard I", "number":"AB273328-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB273328-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"3-Phosphoglycerate", "number":"AB273328-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoglycerate dehydrogenase also known as PHGDH is an enzyme that catalyzes the conversion of 3-phosphoglycerate to 3-phosphohydroxypyruvate. It has an approximate mass of 57 kDa. The PHGDH is located in the cytoplasm and mitochondria where it plays a role in serine biosynthesis. Malate dehydrogenase might also get mentioned together with PHGDH because both enzymes link to metabolic processes. The expression of PHGDH is noted in various tissues with higher levels observed in some cancer cells.
Biological function summary

PHGDH is essential for the serine synthesis pathway and cellular metabolism regulation. It does not form part of large complexes but operates independently to convert substrates necessary for cell growth and proliferation. The enzyme supports the synthesis of nucleotides and proteins impacting cell cycle and survival. Researchers investigate PHGDH to understand its role in metabolic reprogramming especially in cancer cells.

Pathways

PHGDH is important in the serine biosynthesis pathway which is part of the larger glycolytic process. It connects to pathways that contribute to cellular carbon metabolism and energy production. PHGDH interacts with glutamate dehydrogenase protein (GDH) and also influences one-carbon metabolism and redox balance within the cell. These pathways play important roles in providing building blocks for nucleotide and lipid biosynthesis.

PHGDH has relevance in cancer and neurological disorders. Alterations and overexpression of PHGDH have been linked to oncogenesis and tumor growth particularly in breast cancer. The enzyme also associates with neurodegenerative diseases as proper serine levels are important for neuron function. In both conditions PHGDH becomes connected with glutamate dehydrogenase as alterations can impact amino acid metabolism and neurotransmitter balance. The study of PHGDH inhibitors is gaining attention for therapeutic potentials in these diseases.
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Product protocols

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Target data

Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.
See full target information PHGDH
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Neuro-oncology 27:237-253 PubMed39377369

2024

MYC-dependent upregulation of the de novo serine and glycine synthesis pathway is a targetable metabolic vulnerability in group 3 medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Magretta Adiamah,Bethany Poole,Janet C Lindsey,Sarah Kohe,Alaide Morcavallo,Florence Burté,Rebecca M Hill,Helen Blair,Dean Thompson,Mankaran Singh,Shanel Swartz,Stephen Crosier,Tong Zhang,Oliver D K Maddocks,Andrew Peet,Louis Chesler,Ian Hickson,Ross J Maxwell,Steven C Clifford

mBio 15:e0209724 PubMed39207107

2024

Classical swine fever virus inhibits serine metabolism-mediated antiviral immunity by deacetylating modified PHGDH.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowen Li,Yaoyao Huang,Xueyi Liu,Lihong Zhang,Xinyan Wang,Feifan Zhao,Linke Zou,Keke Wu,Wenxian Chen,Yuwei Qin,Sen Zeng,Bingke Li,Yintao He,Yiwan Song,Zhaoyao Li,Jindai Fan,Mingqiu Zhao,Lin Yi,Hongxing Ding,Shuangqi Fan,Jinding Chen

Science signaling 16:eadd6527 PubMed37433004

2023

Skewing cPLAα activity toward oxoeicosanoid production promotes neutrophil N2 polarization, wound healing, and the response to sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth D Maus,Daniel J Stephenson,H Patrick Macknight,Ngoc T Vu,L Alexis Hoeferlin,Minjung Kim,Robert F Diegelmann,Xiujie Xie,Charles E Chalfant

Cellular & molecular immunology 19:1263-1278 PubMed36180780

2022

Serine metabolism orchestrates macrophage polarization by regulating the IGF1-p38 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Shan,Penghui Hu,Lina Ni,Long Shen,Yanan Zhang,Zemin Ji,Yan Cui,Meihua Guo,Haoan Wang,Liyuan Ran,Kun Yang,Ting Wang,Lei Wang,Bin Chen,Zhi Yao,Yingjie Wu,Qiujing Yu
View all publications
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Product promise

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