Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) ab252890 provides a quick and easy way for monitoring PGK activity in various samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Biomedicines 11:2023Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZoran Minic,Yingxi Li,Nico Hüttmann,Gurcharan K Uppal,Rochelle D'Mello,Maxim V BerezovskiPubMed 37189694
- Molecular metabolism 62:1015132022Calcium/calmodulin-dependent protein kinase kinase 2 regulates hepatic fuel metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrittany A Stork,Adam Dean,Andrea R Ortiz,Pradip Saha,Nagireddy Putluri,Maricarmen D Planas-Silva,Iqbal Mahmud,Kimal Rajapakshe,Cristian Coarfa,Stefan Knapp,Philip L Lorenzi,Bruce E Kemp,Benjamin E Turk,John W Scott,Anthony R Means,Brian YorkPubMed 35562082
- Frontiers in oncology 11:6824612021PGK1 Is a Key Target for Anti-Glycolytic Therapy of Ovarian Cancer: Based on the Comprehensive Analysis of Glycolysis-Related Genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Gou,Yuexin Hu,Ouxuan Liu,Hui Dong,Lingling Gao,Shuang Wang,Mingjun Zheng,Xiao Li,Bei LinPubMed 34277429
- Frontiers in oncology 11:6444922021The Neurogenic Compound P7C3 Regulates the Aerobic Glycolysis by Targeting Phosphoglycerate Kinase 1 in Glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjin Chen,Weiqiang Jia,Cuiying Wu,Lihua Chen,Kai Sun,Ji Wang,Boyun Ding,Ning Liu,Ruxiang XuPubMed 34221965
- International journal of molecular sciences 21:2020Freezing Tolerance of Introgression Forms is Associated with the High Activity of Antioxidant System and Adjustment of Photosynthetic Activity under Cold Acclimation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam Augustyniak et. al.PubMed 32824486
- International journal of molecular sciences 21:2020Adjustment of Photosynthetic and Antioxidant Activities to Water Deficit Is Crucial in the Drought Tolerance of Introgression Forms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarzyna Lechowicz et. al.PubMed 32781659
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Sensitivity
- = 50 mU/well
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).
Alternative names
PGKA, MIG10, OK/SW-cl.110, PGK1, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2
What's included?
Recommended products
Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) ab252890 provides a quick and easy way for monitoring PGK activity in various samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 50 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) ab252890 provides a quick and easy way for monitoring PGK activity in various samples. In the first step of this enzymatic assay, PGK converts 3-Phosphoglycerate and ATP to 1,3-Bisphosphoglycerate and ADP. The nascent intermediate is detected via a series of enzymatic reactions that lead the oxidation of NADH to NAD , which can be easily detected (OD= 340 nm). The assay is simple, sensitive and can detect Phosphoglycerate Kinase Activity lower than 50 mU in variety of samples.
This product is manufactured by BioVision, an Abcam company and was previously called K194 Phosphoglycerate Kinase Activity Assay Kit (Colorimetric). K194-100 is the same size as the 100 test size of ab252890.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PGK1 also known as phosphoglycerate kinase-1 is an enzyme involved in glycolysis with a mass of approximately 45 kDa. PGK1 catalyzes the reversible transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP. This enzyme is expressed ubiquitously across various tissues with significant roles in energy metabolism. In yeast it is referred to simply as phosphoglycerate kinase where its function in cellular respiration is widely studied.
- Biological function summary
The PGK1 protein plays an essential role in energy production by participating in the glycolytic pathway. This enzyme functions independently and is not part of any complex. By converting 13-bisphosphoglycerate to 3-phosphoglycerate it supports cellular processes that require ATP. Such activity facilitates the high energy needs of cells ensuring proper cellular function and homeostasis.
- Pathways
The enzyme PGK1 operates predominantly within the glycolytic and gluconeogenesis pathways. Throughout glycolysis it partners with enzymes like phosphofructokinase and pyruvate kinase facilitating the breakdown of glucose into pyruvate while generating ATP. In gluconeogenesis PGK1 aids in glucose formation which helps balance energy dynamics particularly in tissues like the liver and kidney.
- Associated diseases and disorders
PGK1 has associations with tumor growth and proliferation in various cancers. Its elevated expression is often found in tumors linking it to oncogenesis due to the increased demand for glycolytic activity. Additionally mutations in PGK1 can result in phosphoglycerate kinase deficiency a disorder affecting red blood cell metabolism. This links it to related proteins involved in managing metabolic pathways of energy production in affected tissues.
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3 product images
Functional Studies - Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) (ab252890)
Relative PGK Activity was calculated in lysates prepared from rat liver (2 μg), rat muscle (0.5 μg), HeLa (Human epithelial cell line from cervix adenocarcinoma) (1.5 μg) and Jurkat (Human T cell leukemia cell line from peripheral blood) (1.5 μg).
Functional Studies - Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) (ab252890)
Kinetic measurement of Phosphoglycerate Kinase activity from various samples.
Functional Studies - Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) (ab252890)
NADH standard curve.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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