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AB252890

Phosphoglycerate Kinase Activity Assay Kit (Colorimetric)

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(6 Publications)

Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) ab252890 provides a quick and easy way for monitoring PGK activity in various samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

PGKA, MIG10, OK/SW-cl.110, PGK1, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2

3 Images
Enzyme activity assay - Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) (AB252890)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) (AB252890)

Relative PGK Activity was calculated in lysates prepared from rat liver (2 μg), rat muscle (0.5 μg), HeLa (Human epithelial cell line from cervix adenocarcinoma) (1.5 μg) and Jurkat (Human T cell leukemia cell line from peripheral blood) (1.5 μg).

Enzyme activity assay - Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) (AB252890)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) (AB252890)

NADH standard curve.

Enzyme activity assay - Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) (AB252890)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) (AB252890)

Kinetic measurement of Phosphoglycerate Kinase activity from various samples.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Sensitivity

= 50 mU/well

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

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Product details

Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) ab252890 provides a quick and easy way for monitoring PGK activity in various samples. In the first step of this enzymatic assay, PGK converts 3-Phosphoglycerate and ATP to 1,3-Bisphosphoglycerate and ADP. The nascent intermediate is detected via a series of enzymatic reactions that lead the oxidation of NADH to NAD , which can be easily detected (OD= 340 nm). The assay is simple, sensitive and can detect Phosphoglycerate Kinase Activity lower than 50 mU in variety of samples.

This product is manufactured by BioVision, an Abcam company and was previously called K194 Phosphoglycerate Kinase Activity Assay Kit (Colorimetric). K194-100 is the same size as the 100 test size of ab252890.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGK1 also known as phosphoglycerate kinase-1 is an enzyme involved in glycolysis with a mass of approximately 45 kDa. PGK1 catalyzes the reversible transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP. This enzyme is expressed ubiquitously across various tissues with significant roles in energy metabolism. In yeast it is referred to simply as phosphoglycerate kinase where its function in cellular respiration is widely studied.
Biological function summary

The PGK1 protein plays an essential role in energy production by participating in the glycolytic pathway. This enzyme functions independently and is not part of any complex. By converting 13-bisphosphoglycerate to 3-phosphoglycerate it supports cellular processes that require ATP. Such activity facilitates the high energy needs of cells ensuring proper cellular function and homeostasis.

Pathways

The enzyme PGK1 operates predominantly within the glycolytic and gluconeogenesis pathways. Throughout glycolysis it partners with enzymes like phosphofructokinase and pyruvate kinase facilitating the breakdown of glucose into pyruvate while generating ATP. In gluconeogenesis PGK1 aids in glucose formation which helps balance energy dynamics particularly in tissues like the liver and kidney.

PGK1 has associations with tumor growth and proliferation in various cancers. Its elevated expression is often found in tumors linking it to oncogenesis due to the increased demand for glycolytic activity. Additionally mutations in PGK1 can result in phosphoglycerate kinase deficiency a disorder affecting red blood cell metabolism. This links it to related proteins involved in managing metabolic pathways of energy production in affected tissues.
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Product protocols

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Target data

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed : 30323285, PubMed : 7391028). In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed : 2324090). May play a role in sperm motility (PubMed : 26677959).
See full target information PGK1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed37189694

2023

Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Zoran Minic,Yingxi Li,Nico Hüttmann,Gurcharan K Uppal,Rochelle D'Mello,Maxim V Berezovski

Molecular metabolism 62:101513 PubMed35562082

2022

Calcium/calmodulin-dependent protein kinase kinase 2 regulates hepatic fuel metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany A Stork,Adam Dean,Andrea R Ortiz,Pradip Saha,Nagireddy Putluri,Maricarmen D Planas-Silva,Iqbal Mahmud,Kimal Rajapakshe,Cristian Coarfa,Stefan Knapp,Philip L Lorenzi,Bruce E Kemp,Benjamin E Turk,John W Scott,Anthony R Means,Brian York

Frontiers in oncology 11:682461 PubMed34277429

2021

PGK1 Is a Key Target for Anti-Glycolytic Therapy of Ovarian Cancer: Based on the Comprehensive Analysis of Glycolysis-Related Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Gou,Yuexin Hu,Ouxuan Liu,Hui Dong,Lingling Gao,Shuang Wang,Mingjun Zheng,Xiao Li,Bei Lin

Frontiers in oncology 11:644492 PubMed34221965

2021

The Neurogenic Compound P7C3 Regulates the Aerobic Glycolysis by Targeting Phosphoglycerate Kinase 1 in Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjin Chen,Weiqiang Jia,Cuiying Wu,Lihua Chen,Kai Sun,Ji Wang,Boyun Ding,Ning Liu,Ruxiang Xu

International journal of molecular sciences 21: PubMed32824486

2020

Freezing Tolerance of Introgression Forms is Associated with the High Activity of Antioxidant System and Adjustment of Photosynthetic Activity under Cold Acclimation.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Augustyniak,Izabela Pawłowicz,Katarzyna Lechowicz,Karolina Izbiańska-Jankowska,Magdalena Arasimowicz-Jelonek,Marcin Rapacz,Dawid Perlikowski,Arkadiusz Kosmala

International journal of molecular sciences 21: PubMed32781659

2020

Adjustment of Photosynthetic and Antioxidant Activities to Water Deficit Is Crucial in the Drought Tolerance of Introgression Forms.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Lechowicz,Izabela Pawłowicz,Dawid Perlikowski,Magdalena Arasimowicz-Jelonek,Sara Blicharz,Aleksandra Skirycz,Adam Augustyniak,Robert Malinowski,Marcin Rapacz,Arkadiusz Kosmala
View all publications
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