Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) (ab252890)

Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) ab252890 provides a quick and easy way for monitoring PGK activity in various samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

This product has no reviews yet! Submit a review

Alternative names=Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA, OK/SW-cl.110, MIG10

Detection method: Colorimetric
Sample types: Tissue, Suspension cells, Adherent cells
Sensitivity: = 50 mU/well

Reactivity data

Application	Reactivity	Dilution info	Notes
Application FuncS	Reactivity Reacts	Dilution info -	Notes -

Images

Target data

PGK1

What's included?

100 Test

Components

3-Phosphoglycerate

1 x 1 Vial

96-Well UV Transparent Plate

1 x 1 Unit

ATP III

2 x 1 Vial

Assay Buffer IV

1 x 25 mL

NADH I

1 x 1 Vial

PGK Developer

1 x 1 Vial

PGK Positive Control

1 x 1 Vial

Recommended products

Alternative names=Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA, OK/SW-cl.110, MIG10

Key facts

Detection method: Colorimetric
Sample types: Tissue, Suspension cells, Adherent cells
Assay Platform: Microplate (12 x 8 well strips)
Sensitivity: = 50 mU/well

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: -20°C
Appropriate long-term storage conditions: -20°C
Storage information: -20°C

Notes

Phosphoglycerate Kinase Activity Assay Kit (Colorimetric) ab252890 provides a quick and easy way for monitoring PGK activity in various samples. In the first step of this enzymatic assay, PGK converts 3-Phosphoglycerate and ATP to 1,3-Bisphosphoglycerate and ADP. The nascent intermediate is detected via a series of enzymatic reactions that lead the oxidation of NADH to NAD , which can be easily detected (OD= 340 nm). The assay is simple, sensitive and can detect Phosphoglycerate Kinase Activity lower than 50 mU in variety of samples.

This product is manufactured by BioVision, an Abcam company and was previously called K194 Phosphoglycerate Kinase Activity Assay Kit (Colorimetric). K194-100 is the same size as the 100 test size of ab252890.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Visit the general protocols
Visit troubleshooting

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Products

Resources

Support

About us

Phosphoglycerate Kinase Activity Assay Kit (Colorimetric)

Reactivity data

Images

What's included?

Recommended products