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AB273343

Phospholipase C Activity Assay Kit (Colorimetric)

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(1 Publication)

Phospholipase C Activity Assay Kit (Colorimetric) (ab273343) is a direct assay to determine the Phospholipase C (PLC) activity in various biological samples.

View Alternative Names

Phospholipase C, PLC, Cereolysin A, Phosphatidylcholine cholinephosphohydrolase, plc

3 Images
Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)
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Supplier Data

Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)

Example data.

PLC Standard Curve.

Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)
  • FuncS

Supplier Data

Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)

Example data.

PLC activity detected in rat brain, kidney and muscle lysates.

Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)
  • FuncS

Supplier Data

Functional Studies - Phospholipase C Activity Assay Kit (Colorimetric) (AB273343)

Example data.

Reaction curve of the PLC activity.

Key facts

Detection method

Colorimetric

Sample types

Tissue

Assay type

Enzyme activity

Results type

Quantitative

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospholipase C Activity Assay Kit (Colorimetric) (ab273343) is a direct assay to determine the Phospholipase C (PLC) activity in various biological samples. The assay uses a specific PLC chromogenic substrate to detect PLC activity and the generated final product can be measured colorimetrically at OD 405 nm. The OD signal is proportional to the PLC activity.

The assay is rapid, sensitive and is a convenient tool for detecting PLC activity.

It can detect as low as 0.1 mU PLC activity under the assay conditions.

This product is manufactured by BioVision, an Abcam company and was previously called K2016 Phospholipase C Activity Assay Kit (Colorimetric). K2016-100 is the same size as the 100 test size of ab273343.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273343-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"PLC Substrate", "number":"AB273343-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PLC Enzyme", "number":"AB273343-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"p-Nitrophenol Standard", "number":"AB273343-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 61", "number":"AB273343-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C also known as PLC is an enzyme that catalyzes the hydrolysis of phosphatidylinositol 45-bisphosphate (PIP2) into diacylglycerol (DAG) and inositol trisphosphate (IP3). It has several isoforms such as PLC beta and PLC gamma each with different molecular sizes. For example PLC gamma typically has a mass of about 130 kDa. This enzyme is expressed in many tissues including the brain liver and pancreas where it plays an important role in cellular signaling.
Biological function summary

The activity of Phospholipase C generates second messengers that are important for intracellular signal transduction. It functions as part of larger signaling complexes and can be involved in regulating various cellular processes like proliferation differentiation and apoptosis. This role makes it an important regulator in many physiological responses. The enzyme often interacts with membrane-bound receptors linking extracellular signals to internal cellular responses.

Pathways

Phospholipase C is an important player in the phosphatidylinositol signaling pathway and is interconnected with the calcium signaling pathway. Within these pathways PLC activity triggers the release of calcium from internal stores which in turn can activate calcium-dependent proteins such as protein kinase C. These interactions aid in modulating cellular responses to external stimuli.

PLC abnormalities have been linked to conditions like cancer and bipolar disorder. Altered PLC function can disrupt normal cellular signaling contributing to the pathogenesis of these diseases. In cancer abnormal PLC activity is tied to uncontrolled cell growth often interacting with proteins like Ras to drive oncogenic processes. In bipolar disorder disruptions in PLC function affect neurotransmitter pathways implicating changes in proteins associated with synaptic signaling.
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Product protocols

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Target data

Required, with sphingomyelinase, to effect target cell lysis (hemolysis).
See full target information plc
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 24:516-530 PubMed36732424

2023

Phosphoinositide acyl chain saturation drives CD8 effector T cell signaling and function.

Applications

Unspecified application

Species

Unspecified reactive species

Joy Edwards-Hicks,Petya Apostolova,Joerg M Buescher,Hannes Maib,Michal A Stanczak,Mauro Corrado,Ramon I Klein Geltink,Maria Elena Maccari,Matteo Villa,Gustavo E Carrizo,David E Sanin,Francesc Baixauli,Beth Kelly,Jonathan D Curtis,Fabian Haessler,Annette Patterson,Cameron S Field,George Caputa,Ryan L Kyle,Melanie Soballa,Minsun Cha,Harry Paul,Jacob Martin,Katarzyna M Grzes,Lea Flachsmann,Michael Mitterer,Liang Zhao,Frances Winkler,David Ali Rafei-Shamsabadi,Frank Meiss,Bertram Bengsch,Robert Zeiser,Daniel J Puleston,David O'Sullivan,Edward J Pearce,Erika L Pearce
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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