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AB234050

Phospholipid Assay Kit (Colorimetric/Fluorometric)

5

(1 Review)

|

(5 Publications)

Phospholipid Assay Kit (Colorimetric/Fluorometric) (ab234050) is a simple, accurate and reproducible way to measure total choline-containing phospholipids in biological samples such as serum and plasma.
6 Images
Functional Studies - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)
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Supplier Data

Functional Studies - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)
Functional Studies - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)
  • FuncS

Supplier Data

Functional Studies - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)
Functional Studies - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)
  • FuncS

Supplier Data

Functional Studies - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)
Functional Studies - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)
  • FuncS

Supplier Data

Functional Studies - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)

Determination of Phospholipid Concentration in Human Serum and plasma.

Normal concentrations in human ranges between 1 and 4 mM.

Functional Studies - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)
  • FuncS

Supplier Data

Functional Studies - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)
Schematic Diagram - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Phospholipid Assay Kit (Colorimetric/Fluorometric) (AB234050)

Phospholipids containing choline are hydrolyzed by the PC Hydrolysis Enzyme. The choline is used by the Enzyme Mix IV along with the OxiRed Probe resulting in a colorimetric product with an absorbance at 570 nm and fluorometric excitation/emission at 535/587 nm.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Plasma, Serum

Results type

Quantitative

Assay Platform

Microplate reader

Product details

Phospholipid Assay Kit (Colorimetric/Fluorometric) (ab234050) is a simple, accurate and reproducible way to measure total choline-containing phospholipids in biological samples such as serum and plasma.

This kit provides a high-throughput assay of inhibitors/inducers affecting phospholipid metabolism. The signal is directly proportional to the phospholipid concentration of phospholipids in the sample and can detect phospholipids in the range of 0.1 to 10 nmol per sample.

This product is manufactured by BioVision, an Abcam company and was previously called K351 Phospholipid Assay Kit (Colorimetric/Fluorometric). K351-100 is the same size as the 100 test size of ab234050.

Phospholipids are a major component of lipid bilayer membranes. In addition, phospholipids are biologically important molecules as they are closely involved in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline moiety. In human serum and plasma, almost 95% of the phospholipids are choline-containing phospholipids such as phosphatidylcholine and sphingomyelin. The remaining 5% is mainly choline-free phospholipids (i.e. phosphatidylethanolamine and phosphatidylserine). Phospholipid concentration is considered a disease biomarker as it can be altered in many lung, liver and heart diseases.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab234050-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Assay Buffer 1", "number":"AB234050-CMP01", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB234050-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PC Hydrolysis Enzyme", "number":"AB234050-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Phosphatidylcholine Standard", "number":"AB234050-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Enzyme Mix IV", "number":"AB234050-CMP03", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipids are essential structural components of cellular membranes also known as glycerophospholipids. Their structure typically consists of a hydrophilic head and two hydrophobic tails composed of fatty acids. Phospholipids have a molecular mass range because of their varying fatty acid chains but generally fall around 750 Daltons. They are universally expressed in all cell types from bacteria to eukaryotes due to their fundamental role in membrane formation. Phospholipids also serve as substrates for several signaling molecules adding complexity to their function.
Biological function summary

Phospholipids are vital in maintaining the integrity and functionality of membranes. They form lipid bilayers providing impermeable barriers for cells and organelles. Phospholipid molecules participate in cell signaling serving as precursors for signaling lipids like phosphatidylinositol. These molecules can organize into complex structures known as liposomes or micelles depending on their environment. In addition they interact extensively within membrane proteins affecting the protein's structure and function.

Pathways

Phospholipids play a significant role in the signal transduction and apoptosis pathways. In the signal transduction pathway phosphatidylinositol derivatives act as secondary messengers regulating various cellular responses. Proteins such as phospholipase C and kinase enzymes interact closely with these derivatives illustrating their involvement in complex cellular processes. The apoptosis pathway also relies on phospholipids with the release of ceramide from sphingomyelin degradation triggering programmed cell death. These interactions highlight important aspects of cellular regulation and homeostasis.

Phospholipids are linked to cardiovascular diseases and metabolic disorders. Imbalances or mutations affecting phospholipid synthesis can lead to atherosclerosis where plaque formation in arteries occurs. Disorders such as Niemann-Pick disease result from dysfunctional phospholipid metabolism specifically involving the protein acid sphingomyelinase. Its dysfunction leads to abnormal lipid accumulation in tissues. The understanding and manipulation of phospholipid pathways offer potential therapeutic approaches for these diseases.

Product protocols

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:4146-4161 PubMed39247829

2024

N6-methyladenosine modification of LATS2 promotes hepatoblastoma progression by inhibiting ferroptosis through the YAP1/ATF4/PSAT1 axis.

Applications

Unspecified application

Species

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Guoqing Zhu,Yi Xie,Zhixuan Bian,Ji Ma,Ni Zhen,Tianshu Chen,Jiabei Zhu,Siwei Mao,Xiaochen Tang,Li Liu,Song Gu,Miao Ding,Qiuhui Pan

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2104291 PubMed36031387

2022

Trip13 Depletion in Liver Cancer Induces a Lipogenic Response Contributing to Plin2-Dependent Mitotic Cell Death.

Applications

Unspecified application

Species

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Marcos Rios Garcia,Bettina Meissburger,Jessica Chan,Roldan M de Guia,Frits Mattijssen,Stephanie Roessler,Andreas L Birkenfeld,Nathanael Raschzok,Fabien Riols,Janina Tokarz,Maude Giroud,Manuel Gil Lozano,Goetz Hartleben,Peter Nawroth,Mark Haid,Miguel López,Stephan Herzig,Mauricio Berriel Diaz

Frontiers in plant science 12:727596 PubMed34646287

2021

Melatonin Alleviates Low-Temperature Stress ABI5-Mediated Signals During Seed Germination in Rice ( L.).

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Ruiqing Li,Meng Jiang,Yue Song,Huali Zhang

BMC complementary medicine and therapies 21:199 PubMed34261471

2021

Protective effect of Glechoma hederacea extract against gallstone formation in rodent models.

Applications

Unspecified application

Species

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Min Xiao,Mengbi Yang,Xiaoyu Ji,Dan Li,Yuning Xie,Yuanfeng Lyu,Zhong Zuo

Biochimica et biophysica acta. Molecular basis of 1866:165972 PubMed32949768

2020

Age-related and atherosclerosis-related erythropathy in ApoE/LDLR mice.

Applications

Unspecified application

Species

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Jakub Dybas,Katarzyna Bulat,Aneta Blat,Tasnim Mohaissen,Aleksandra Wajda,Mateusz Mardyla,Magdalena Kaczmarska,Magdalena Franczyk-Zarow,Kamilla Malek,Stefan Chlopicki,Katarzyna M Marzec
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