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AB150681

Picro Sirius Red Stain Kit (Connective Tissue Stain)

5

(2 Reviews)

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(432 Publications)

Picro-Sirius Red Stain Kit (Connective Tissue Stain) ab150681 is an easy-to-use histological kit to visualize collagen I and collagen III fibers in addition to muscle in tissue sections.

- Specific stain to study collagen networks in different tissues
- Suitable for light microscopy and polarized light microscopy
- Cited in over 320 publications
4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681)

ab150681 (Picro Sirius Red stain) staining collagen (red), muscle fibres (yellow), cytoplasm (yellow) and red blood cells (yellow) in formalin fixed paraffin embedded normal human colon.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681)

ab150681 Picro Sirius Red Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human skeletal muscle.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681)

ab150681 staining mouse liver sections in control (saline group) showing normal lobular structure; in CCl4 treated showing extensive collagen deposition and bridging fibrosis; and in CCl4-AND treated mice which showed decreased collagen deposition.

Kumar P et al. PLoS One (2014) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681)

Evaluation of liver tissues fibrosis pattern in IUGR and SHAM adult males rats, using Picro-Sirius Red Stain (ab150681). Values are expressed as fold change (mean + SD, * p<0.01).

This image is taken from Deodati A. et al. PLoS One. 2018; 13(6): e0198490. Fig 3e. doi: 10.1371/journal.pone.0198490. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

Key facts

Sample types

Tissue sections

Assay time

1h

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Product details

Picro sirius red staining has traditionally sometimes been used with polarized light to more easily differentiate the collagen fibres from the background. Studies have used the differences between the appearance of different collagen fibres under polarized light to differentiate between different types of collagen fibres; it is unclear if the differences in appearance of the fibres is due to orientation, thickness and packing of the fibres rather than reflecting different types of collagen fibers.

Picro sirius red staining protocol summary

  • - Deparaffinize sections if necessary and hydrate in distilled water
  • - Cover sections in picro-sirius red solution and incubate for 60 min
  • - Wash slide with acetic acid solution
  • - Wash slide with absolute alcohol
  • - Dehydrate, clear and mount slide

Staining Interpretation

Light Microscopy

Collagen Red
Muscle Fibers Yellow
Cytoplasm Yellow

Polarized Light Microscopy

Type I (Thick fibers) Yellow-Orange Birefringence
Type III (Thin fibers) Green Birefringence

Control Tissue: Lung, Uterus, Muscle, Kidney.

Getting the best performance from ab150681
ab150681 may not be suitable for frozen sections.

Related and recommended products
See below other connective tissue stains to highlight and identify different types of connective tissue in tissue samples with different dyes:

  • - Trichrome Stain Kit (Connective Tissue Stain) ab150686
  • - Phosphotungstic Acid Hematoxylin (PTAH) Stain Kit (Connective Tissue Stain) ab150683
  • - Reticulum Stain Kit (Connective Tissue Stain) ab150684

Other Notes

Please note, the 250 mL selling size, was previously described as 100 Test. There has been no change to the physical product.

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What's included?

{ "values": { "250mL": { "sellingSize": "250 mL", "publicAssetCode":"ab150681-250mL", "assetComponentDetails": [ { "size":"2 x 250 mL", "name":"Acetic Acid Solution", "number":"AB150681-CMP01" }, { "size":"1 x 250 mL", "name":"Picro Sirius Red Solution", "number":"AB150681-CMP02" } ] }, "30mL": { "sellingSize": "30 mL", "publicAssetCode":"ab150681-30mL", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Picro Sirius Red Solution", "number":"AB150681-CMP02", "productcode":"" }, { "size":"2 x 30 mL", "name":"Acetic Acid Solution", "number":"AB150681-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen I and III are important structural proteins within the extracellular matrix providing tensile strength and structural support to tissues. Collagen I often called Type I collagen has a molecular mass of approximately 300 kDa. It is expressed abundantly in skin bone tendons and other connective tissues. Collagen III known as Type III collagen is generally found alongside Type I particularly in skin blood vessels and internal organs. Both collagens are essential for maintaining tissue integrity with muscle fibers interacting closely to support proper muscle function.
Biological function summary

Collagen fibers are significant for tissue architecture and are involved in wound healing fibrosis and tissue regeneration. They are components of a complex network within the extracellular matrix that involves proteoglycans and other matrix proteins. Collagens also serve as ligands for cellular receptors like integrins which influence cell adhesion and signaling processes. Picrosirius red is a commonly used stain for identifying collagen fibers in histological studies as it enhances the visibility of these fibers under polarized microscopy.

Pathways

These collagens play roles in both the Wnt signaling pathway and the transforming growth factor-beta (TGF-β) pathway. They are important for tissue development and repair directly interacting with factors like fibronectin and matrix metalloproteinases. The interactions within these pathways regulate not only synthesis and degradation of the extracellular matrix but also influence cellular behaviors such as migration proliferation and differentiation.

Collagen I and III are associated with fibrotic diseases and connective tissue disorders. Mutations or dysregulations in collagen production can lead to conditions like Ehlers-Danlos syndrome characterized by connective tissue fragility or pulmonary fibrosis which involves excessive deposition of extracellular matrix components. Aberrations in these collagens can also disrupt interactions with proteins like elastin impacting tissue elasticity and leading to further pathological conditions.
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Product protocols

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Target data

See full target information Direct Red 80
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Publications (432)

Recent publications for all applications. Explore the full list and refine your search

Bone & joint research 14:568-577 PubMed40545249

2025

Overcoming the detrimental impact of volumetric muscle loss on segmental fracture healing via the induced membrane technique.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew R Clark,Michael S Valerio,Jonathan Kulwatno,Sergey S Kanovka,Andrew L Ferrer,Christopher L Dearth,Stephen M Goldman

Frontiers in pharmacology 16:1562116 PubMed40510419

2025

Therapeutic potential of cultivated with seeds for alleviating western diet-induced type 2 diabetes and diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Shinn-Zong Lin,Wei-Wen Kuo,Bruce Chi-Kang Tsai,Catherine Reena Paul,Chia-Hua Kuo,Dennis Jine-Yuan Hsieh,Shih-Wen Kao,Pei-Ying Pai,Shan-Jun Chen,Chih-Yang Huang,Kuan-Ho Lin

International journal of molecular sciences 26: PubMed40429870

2025

Spinach Extract Reduces Kidney Damage in Diabetic Rats by Impairing the AGEs/RAGE Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Flores-Estrada,Agustina Cano-Martínez,Luz Ibarra-Lara,Adriana Jiménez,Carmen Palacios-Reyes,Luis J Pinto García,María G Ortiz-López,Olga Nelly Rodríguez-Peña,Luis Barbo Hernández-Portilla

Nature communications 16:4745 PubMed40399382

2025

Multiomic analysis of human kidney disease identifies a tractable inflammatory and pro-fibrotic tubular cell phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian Reck,David P Baird,Stefan Veizades,Callum Sutherland,Rachel M B Bell,Heeyoun Hur,Carolynn Cairns,Piotr P Janas,Ross Campbell,Andy Nam,Wei Yang,Nathan Schurman,Claire Williams,Eoin O'Sullivan,Meryam Beniazza,Andrea Corsinotti,Christopher Bellamy,Jeremy Hughes,Alexander Laird,Laura Denby,Tamir Chandra,David A Ferenbach,Bryan R Conway

Experimental animals : PubMed40368825

2025

Comparison of the characteristics of non-alcoholic fatty liver disease in Lepr and Lepr mice.

Applications

Unspecified application

Species

Unspecified reactive species

Seungwoo Lee,Jae-Hong Min,Myoung Jun Kim,Somi Yun,Min Kyoung Seo,Jong Kwon Lee

European heart journal open 5:oeaf021 PubMed40352252

2025

Fluoroquinolones and the risk of aortic aneurysm or aortic dissection: evidence from a nationwide nested case-control study paralleled with matched experimental models.

Applications

Unspecified application

Species

Unspecified reactive species

Callan D Wesley,Jarl Emanuel Strange,Anders Holt,Gunnar H Gislason,Cédric H G Neutel,Dustin N Krüger,Celine Civati,Mart Theunis,Tania Naessens,Lynn Roth,Guido R Y De Meyer,Wim Martinet,Peter Vibe Rasmussen,Pieter-Jan Guns

FASEB bioAdvances 7:e70009 PubMed40330431

2025

Furin inhibits HSCs activation and ameliorates liver fibrosis by regulating PTEN-L/PINK1/parkin mediated mitophagy in mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Wei Song,Yu-Hua Zhu,Ming-Ze Ma

The Journal of comparative neurology 533:e70055 PubMed40293445

2025

Molecular and Morphological Circuitry of the Octopus Sucker Ganglion.

Applications

Unspecified application

Species

Unspecified reactive species

Cassady S Olson,Aashna Moorjani,Clifton W Ragsdale

Science advances 11:eadn5409 PubMed40267206

2025

Functional tendon regeneration is driven by regulatory T cells and IL-33 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Varun Arvind,Giulia Crosio,Kristen Howell,Hui Zhang,Angela Montero,Alice H Huang

Langenbeck's archives of surgery 410:130 PubMed40240627

2025

Transversalis fascia collagen content and the risk of surgical complications: results of a prospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Marcin Morawski,Maciej Krasnodębski,Jakub Rochoń,Hubert Kubiszewski,Mikołaj Staszewski,Mikołaj Kuncewicz,Piotr Krawczyk,Paweł Rykowski,Adam Bołtuć,Zbigniew Lewandowski,Wojciech Figiel,Marek Krawczyk,Michał Grąt
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