Picro-Sirius Red Stain Kit (Connective Tissue Stain) ab150681 is an easy-to-use histological kit to visualize collagen I and collagen III fibers in addition to muscle in tissue sections.
- Specific stain to study collagen networks in different tissues
- Suitable for light microscopy and polarized light microscopy
- Cited in over 320 publications
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- EJNMMI research 14:72024Assessment of the alpha 7 nicotinic acetylcholine receptor as an imaging marker of cardiac repair-associated processes using NS14490.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictoria J M Reid,Wesley K X McLoughlin,Kalyani Pandya,Holly Stott,Monika Iškauskienė,Algirdas Šačkus,Judit A Marti,Dominic Kurian,Thomas M Wishart,Christophe Lucatelli,Dan Peters,Gillian A Gray,Andrew H Baker,David E Newby,Patrick W F Hadoke,Adriana A S Tavares,Mark G MacAskillPubMed 38206500
- International journal of molecular sciences 25:2024Beta Blockade Prevents Cardiac Morphological and Molecular Remodelling in Experimental Uremia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanmugakumar Chinnappa,Azhar Maqbool,Hema Viswambharan,Andrew Mooney,Laura Denby,Mark DrinkhillPubMed 38203544
- Frontiers in molecular biosciences 10:12639132024Transcriptional changes during isoproterenol-induced cardiac fibrosis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDisha Nanda,Priyanka Pant,Pratheusa Machha,Divya Tej Sowpati,Regalla KumarswamyPubMed 38178867
- iScience 26:1085212024Scaffold-induced compression enhances ligamentization potential of decellularized tendon graft reseeded with ACL-derived cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinsung Park,Hyunsoo Soh,Sungsin Jo,Subin Weon,Seung Hoon Lee,Jeong-Ah Park,Myung-Kyu Lee,Tae-Hwan Kim,Il-Hoon Sung,Jin Kyu LeePubMed 38162024
- Clinical and translational medicine 13:e15132023Molecular analysis of XPO1 inhibitor and gemcitabine-nab-paclitaxel combination in KPC pancreatic cancer mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMd Hafiz Uddin,Mohammad Najeeb Al-Hallak,Husain Yar Khan,Amro Aboukameel,Yiwei Li,Sahar F Bannoura,Gregory Dyson,Seongho Kim,Yosef Mzannar,Ibrahim Azar,Tanya Odisho,Amr Mohamed,Yosef Landesman,Steve Kim,Rafic Beydoun,Ramzi M Mohammad,Philip A Philip,Anthony F Shields,Asfar S AzmiPubMed 38131168
- Pharmaceuticals (Basel, Switzerland) 16:2023Mitigating Scar Tissue Formation in Tendon Injuries: Targeting HMGB1, AMPK Activation, and Myofibroblast Migration All at Once.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianying Zhang,Roshawn Brown,MaCalus V Hogan,James H-C WangPubMed 38139865
- Nature cell biology 25:1804-18202023COPI vesicle formation and N-myristoylation are targetable vulnerabilities of senescent cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDomhnall McHugh,Bin Sun,Carmen Gutierrez-Muñoz,Fernanda Hernández-González,Massimiliano Mellone,Romain Guiho,Imanol Duran,Joaquim Pombo,Federico Pietrocola,Jodie Birch,Wouter W Kallemeijn,Sanjay Khadayate,Gopuraja Dharmalingam,Santiago Vernia,Edward W Tate,Juan Pedro Martínez-Barbera,Dominic J Withers,Gareth J Thomas,Manuel Serrano,Jesús GilPubMed 38012402
- Scientific reports 13:205772023Superiority of systemic bleomycin to intradermal HOCl for the study of interstitial lung disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArina Morozan,Sydney Joy,Utako Fujii,Richard Fraser,Kevin Watters,James G Martin,Inés ColmegnaPubMed 37996447
- Frontiers in immunology 14:12594342023Myeloid PFKFB3-mediated glycolysis promotes kidney fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuhua Yang,Emily Huo,Yongfeng Cai,Zhidan Zhang,Charles Dong,John M Asara,Huidong Shi,Qingqing WeiPubMed 38035106
- Cancers 15:2023Krüppel-like Factor 5 Plays an Important Role in the Pathogenesis of Chronic Pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaryam Alavi,Ana Mejia-Bautista,Meiyi Tang,Jela Bandovic,Avi Z Rosenberg,Agnieszka B BialkowskaPubMed 38001687
Key facts
- Sample types
- Tissue sections
- Assay time
- 1h
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Picro-Sirius Red Stain Kit (Connective Tissue Stain) ab150681 is an easy-to-use histological kit to visualize collagen I and collagen III fibers in addition to muscle in tissue sections.
- Specific stain to study collagen networks in different tissues
- Suitable for light microscopy and polarized light microscopy
- Cited in over 320 publications
Key facts
- Sample types
- Tissue sections
- Assay time
- 1h
Storage
- Shipped at conditions
- Ambient - Cannot Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- Ambient
Notes
Picro sirius red staining has traditionally sometimes been used with polarized light to more easily differentiate the collagen fibres from the background. Studies have used the differences between the appearance of different collagen fibres under polarized light to differentiate between different types of collagen fibres; it is unclear if the differences in appearance of the fibres is due to orientation, thickness and packing of the fibres rather than reflecting different types of collagen fibers.
Picro sirius red staining protocol summary
- - Deparaffinize sections if necessary and hydrate in distilled water
- - Cover sections in picro-sirius red solution and incubate for 60 min
- - Wash slide with acetic acid solution
- - Wash slide with absolute alcohol
- - Dehydrate, clear and mount slide
Staining Interpretation
Light Microscopy
| Collagen | Red |
| Muscle Fibers | Yellow |
| Cytoplasm | Yellow |
Polarized Light Microscopy
| Type I (Thick fibers) | Yellow-Orange Birefringence |
| Type III (Thin fibers) | Green Birefringence |
Control Tissue: Lung, Uterus, Muscle, Kidney.
Getting the best performance from ab150681
ab150681 may not be suitable for frozen sections.
Related and recommended products
See below other connective tissue stains to highlight and identify different types of connective tissue in tissue samples with different dyes:
- - Trichrome Stain Kit (Connective Tissue Stain) Trichrome Stain Kit (Connective Tissue Stain) ab150686
- - Phosphotungstic Acid Hematoxylin (PTAH) Stain Kit (Connective Tissue Stain) Phosphotungstic Acid Hematoxylin (PTAH) Stain Kit (Connective Tissue Stain) ab150683
- - Reticulum Stain Kit (Connective Tissue Stain) Reticulum Stain Kit (Connective Tissue Stain) ab150684
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen I and III are important structural proteins within the extracellular matrix providing tensile strength and structural support to tissues. Collagen I often called Type I collagen has a molecular mass of approximately 300 kDa. It is expressed abundantly in skin bone tendons and other connective tissues. Collagen III known as Type III collagen is generally found alongside Type I particularly in skin blood vessels and internal organs. Both collagens are essential for maintaining tissue integrity with muscle fibers interacting closely to support proper muscle function.
- Biological function summary
Collagen fibers are significant for tissue architecture and are involved in wound healing fibrosis and tissue regeneration. They are components of a complex network within the extracellular matrix that involves proteoglycans and other matrix proteins. Collagens also serve as ligands for cellular receptors like integrins which influence cell adhesion and signaling processes. Picrosirius red is a commonly used stain for identifying collagen fibers in histological studies as it enhances the visibility of these fibers under polarized microscopy.
- Pathways
These collagens play roles in both the Wnt signaling pathway and the transforming growth factor-beta (TGF-β) pathway. They are important for tissue development and repair directly interacting with factors like fibronectin and matrix metalloproteinases. The interactions within these pathways regulate not only synthesis and degradation of the extracellular matrix but also influence cellular behaviors such as migration proliferation and differentiation.
- Associated diseases and disorders
Collagen I and III are associated with fibrotic diseases and connective tissue disorders. Mutations or dysregulations in collagen production can lead to conditions like Ehlers-Danlos syndrome characterized by connective tissue fragility or pulmonary fibrosis which involves excessive deposition of extracellular matrix components. Aberrations in these collagens can also disrupt interactions with proteins like elastin impacting tissue elasticity and leading to further pathological conditions.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681)
ab150681 (Picro Sirius Red stain) staining collagen (red), muscle fibres (yellow), cytoplasm (yellow) and red blood cells (yellow) in formalin fixed paraffin embedded normal human colon.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681)
ab150681 Picro Sirius Red Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human skeletal muscle.
Kumar P et al. PLoS One (2014) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681)
ab150681 staining mouse liver sections in control (saline group) showing normal lobular structure; in CCl4 treated showing extensive collagen deposition and bridging fibrosis; and in CCl4-AND treated mice which showed decreased collagen deposition.
This image is taken from Deodati A. et al. PLoS One. 2018; 13(6): e0198490. Fig 3e. doi: 10.1371/journal.pone.0198490. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681)
Evaluation of liver tissues fibrosis pattern in IUGR and SHAM adult males rats, using Picro-Sirius Red Stain (ab150681). Values are expressed as fold change (mean + SD, * p<0.01).
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