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Picro-Sirius Red Stain Kit (Connective Tissue Stain) ab150681 is an easy-to-use histological kit to visualize collagen I and collagen III fibers in addition to muscle in tissue sections.

- Specific stain to study collagen networks in different tissues
- Suitable for light microscopy and polarized light microscopy
- Cited in over 320 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (AB150681), expandable thumbnail

Publications

Key facts

Sample types
Tissue sections
Assay time
1h

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

100 Test
Components
Acetic Acid Solution
2 x 250 mL
Picro Sirius Red Solution
1 x 250 mL

Recommended products

Picro-Sirius Red Stain Kit (Connective Tissue Stain) ab150681 is an easy-to-use histological kit to visualize collagen I and collagen III fibers in addition to muscle in tissue sections.

- Specific stain to study collagen networks in different tissues
- Suitable for light microscopy and polarized light microscopy
- Cited in over 320 publications

Key facts

Sample types
Tissue sections
Assay time
1h

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient

Notes

Picro sirius red staining has traditionally sometimes been used with polarized light to more easily differentiate the collagen fibres from the background. Studies have used the differences between the appearance of different collagen fibres under polarized light to differentiate between different types of collagen fibres; it is unclear if the differences in appearance of the fibres is due to orientation, thickness and packing of the fibres rather than reflecting different types of collagen fibers.

Picro sirius red staining protocol summary

  • - Deparaffinize sections if necessary and hydrate in distilled water
  • - Cover sections in picro-sirius red solution and incubate for 60 min
  • - Wash slide with acetic acid solution
  • - Wash slide with absolute alcohol
  • - Dehydrate, clear and mount slide

Staining Interpretation

Light Microscopy

CollagenRed
Muscle FibersYellow
CytoplasmYellow

Polarized Light Microscopy

Type I (Thick fibers)Yellow-Orange Birefringence
Type III (Thin fibers)Green Birefringence

Control Tissue: Lung, Uterus, Muscle, Kidney.

Getting the best performance from ab150681
ab150681 may not be suitable for frozen sections.

Related and recommended products
See below other connective tissue stains to highlight and identify different types of connective tissue in tissue samples with different dyes:

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Collagen I and III are important structural proteins within the extracellular matrix providing tensile strength and structural support to tissues. Collagen I often called Type I collagen has a molecular mass of approximately 300 kDa. It is expressed abundantly in skin bone tendons and other connective tissues. Collagen III known as Type III collagen is generally found alongside Type I particularly in skin blood vessels and internal organs. Both collagens are essential for maintaining tissue integrity with muscle fibers interacting closely to support proper muscle function.

Biological function summary

Collagen fibers are significant for tissue architecture and are involved in wound healing fibrosis and tissue regeneration. They are components of a complex network within the extracellular matrix that involves proteoglycans and other matrix proteins. Collagens also serve as ligands for cellular receptors like integrins which influence cell adhesion and signaling processes. Picrosirius red is a commonly used stain for identifying collagen fibers in histological studies as it enhances the visibility of these fibers under polarized microscopy.

Pathways

These collagens play roles in both the Wnt signaling pathway and the transforming growth factor-beta (TGF-β) pathway. They are important for tissue development and repair directly interacting with factors like fibronectin and matrix metalloproteinases. The interactions within these pathways regulate not only synthesis and degradation of the extracellular matrix but also influence cellular behaviors such as migration proliferation and differentiation.

Associated diseases and disorders

Collagen I and III are associated with fibrotic diseases and connective tissue disorders. Mutations or dysregulations in collagen production can lead to conditions like Ehlers-Danlos syndrome characterized by connective tissue fragility or pulmonary fibrosis which involves excessive deposition of extracellular matrix components. Aberrations in these collagens can also disrupt interactions with proteins like elastin impacting tissue elasticity and leading to further pathological conditions.

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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681)

    ab150681 (Picro Sirius Red stain) staining collagen (red), muscle fibres (yellow), cytoplasm (yellow) and red blood cells (yellow) in formalin fixed paraffin embedded normal human colon.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681)

    ab150681 Picro Sirius Red Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human skeletal muscle.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681), expandable thumbnail
    Kumar P et al. PLoS One (2014) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681)

    ab150681 staining mouse liver sections in control (saline group) showing normal lobular structure; in CCl4 treated showing extensive collagen deposition and bridging fibrosis; and in CCl4-AND treated mice which showed decreased collagen deposition.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681), expandable thumbnail
    This image is taken from Deodati A. et al. PLoS One. 2018; 13(6): e0198490. Fig 3e. doi: 10.1371/journal.pone.0198490. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681)

    Evaluation of liver tissues fibrosis pattern in IUGR and SHAM adult males rats, using Picro-Sirius Red Stain (ab150681). Values are expressed as fold change (mean + SD, * p<0.01).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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