AB139435

PKA Kinase Activity Assay Kit

Name: PKA Kinase Activity Assay Kit (ab139435) | Abcam
Brand: Abcam Limited
SKU: AB139435
Price: 870 USD
Availability: InStock

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(74 Publications)

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Functional Studies - PKA Kinase Activity Assay Kit (AB139435)

FuncS

Lab

Functional Studies - PKA Kinase Activity Assay Kit (AB139435)

1e7 3T3-L1 cells were treated for 15 minutes with 1 μM dexamethasone (ab120743) and 1 μg/mL insulin (ab123768) with the addition of 50 μM forskolin (ab120058), 0.5 mM IBMX (ab120840) or 0.1 mM 8-CPT-cAMP (ab120424). Cell extracts were prepared according to the protocol. Lysates were diluted 50-fold, and tested in duplicates (+/-SD).

FuncS

Lab

Functional Studies - PKA Kinase Activity Assay Kit (AB139435)

Titration of cAMP Protein Kinase (ab56268), background signal subtracted (duplicates; +/- SD).

FuncS

Lab

Functional Studies - PKA Kinase Activity Assay Kit (AB139435)

Titration of positive control (active PKA), background signal subtracted (duplicates; +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Suspension cells, Tissue Extracts, Adherent cells

Assay type

Enzyme activity

Assay time

4h 30m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

PKA Kinase Activity Kit (ab139435) is a non-radioactive assay providing a safe, rapid and reliable method for the screening of inhibitors or activators of PKA and for quantitating the activity of PKA in purified or partially purified enzyme preparations. This kit is based on a solid phase enzyme-linked immuno-absorbent assay (ELISA) that uses a specific synthetic peptide as a substrate for PKA and a polyclonal antibody that recognizes the phosphorylated form of the substrate.

PKA activity assay principle
In the assay, a synthetic peptide is used as PKA specific substrate. The substrate, which is pre-coated on the microplate provided, is phosphorylated by the PKA present in the sample after addition of ATP. The phosphorylated substrate is then recognized by a polyclonal antibody that recognizes only the phospho-substrate.

The phospho-specific antibody is subsequently recognized by an HRP conjugated secondary antibody. The assay is finally developed with TMB substrate and color develops in proportion to the PKA activity existing in the sample.

The color development is stopped with acid stop solution and the intensity of the color is measured in a microplate reader at OD 450 nm.

The assay is designed for the analysis of PKA activity in the solution phase. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols.

The kit offers the following advantages:
1. Safe - non-radioactive measurement of kinase activity
2. Flexible - kinetic and end-point options available
3. Fast - results in < 4.5 hours
4. Efficient - only 30 μl diluted sample needed per well

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 12" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab139435-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer", "number":"AB139435-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody Dilution Buffer", "number":"AB139435-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Anti-Rabbit IgG: HRP Conjugate", "number":"AB139435-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"PKA Phosphospecific Substrate Antibody", "number":"AB139435-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PKA Substrate Microtiter Plate", "number":"AB139435-CMP06", "productcode":"" }, { "size":"1 x 2 mg", "name":"ATP", "number":"AB139435-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution 2", "number":"AB139435-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"Kinase Assay Dilution Buffer", "number":"AB139435-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB139435-CMP09", "productcode":"" }, { "size":"1 x 30 µL", "name":"Active PKA 20 ng/µl", "number":"AB139435-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

MULTI

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein kinase A (PKA) also known as cAMP-dependent protein kinase plays an important role in cellular signaling. It is composed of a regulatory and a catalytic subunit having a molecular mass around 150 kDa. PKA is expressed widely in tissues such as the heart brain and skeletal muscle. The activation of PKA occurs when cyclic adenosine monophosphate (cAMP) binds to its regulatory subunit leading to the release of the catalytic subunit which then phosphorylates various target proteins.

Biological function summary

PKA acts as a significant regulator of gene expression and cellular metabolism. It functions within the PKA signaling complex which includes A-kinase anchoring proteins (AKAPs) that localize PKA to specific areas in the cell aiding in precise function. PKA modulates processes such as lipolysis glycogen metabolism and transcription. Through these activities it contributes to cellular responses to hormonal changes by phosphorylating key substrates.

Pathways

PKA activation integrates into the cAMP signaling pathway an essential regulatory pathway influencing a broad range of physiological responses. This pathway connects PKA with the downstream effectors such as CREB and other transcription factors. Moreover PKA is involved in the MAPK pathway where it interacts with proteins like MEK and ERK orchestrating responses to growth factors. These pathways demonstrate PKA's role in integrating extracellular signals into cellular actions.

Dysregulation of PKA activity associates with conditions like Cushing's syndrome and cardiac hypertrophy. In Cushing's syndrome mutations affecting the regulatory subunit of PKA can lead to unregulated hormone activity. Cardiac hypertrophy involves altered PKA pathway signaling affecting heart muscle growth and function. PKA interacts with proteins such as phospholamban in cardiac disorders impacting calcium handling and heart contractility.

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Product protocols

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Target data

See full target information Protein kinase A

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Publications (74)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4990 PubMed40442088

2025

Pharmacological and physiological activation of TGR5 in the NTS lowers food intake by enhancing leptin-STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kyla Bruce,Song-Yang Zhang,Ameth N Garrido,Melissa T Wang,Tomás P Bachor,Pengcheng Wang,Allison W Xu,Zeyu Yang,Tony K T Lam

Signal transduction and targeted therapy 10:109 PubMed40199859

2025

The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chandana Thimme Gowda,Mallikarjuna Siraganahalli Eshwaraiah,Jiaohong Wang,Youngyi Lim,Maria Lauda Tomasi,Nirmala Mavila,Komal Ramani

eNeuro 12: PubMed39947902

2025

The Dual Role of A2aR in Neuroinflammation: Modulating Microglial Polarization in White Matter Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Cheng,Wenchao Cheng,Yuhan Wang,Xiuying Chen,Lan Zhang,Yi Li,Fa Shen,Dezhi Yuan,Pian Hong,Wen Huang

International journal of molecular sciences 25: PubMed39684198

2024

The Activation of p300 Enhances the Sensitivity of Pituitary Adenomas to Dopamine Agonist Treatment by Regulating the Transcription of DRD2.

Applications

Unspecified application

Species

Unspecified reactive species

Sihan Li,Xingbo Li,Quanji Wang,Qian Jiang,Zihan Wang,Linpeng Xu,Yimin Huang,Ting Lei

Bone research 12:64 PubMed39516484

2024

Inhibition of sympathetic tone via hypothalamic descending pathway propagates glucocorticoid-induced endothelial impairment and osteonecrosis of the femoral head.

Applications

Unspecified application

Species

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Wenkai Shao,Bo Wang,Ping Wang,Shuo Zhang,Song Gong,Xiaodong Guo,Deyu Duan,Zengwu Shao,Weijian Liu,Lei He,Fei Gao,Xiao Lv,Yong Feng

Aging and disease 16:3180-3203 PubMed39500352

2024

Modulation of Mitochondrial Dynamics by the Angiotensin System in Dopaminergic Neurons and Microglia.

Applications

Unspecified application

Species

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Aloia Quijano,Ana I Rodriguez-Perez,María Alicia Costa-Besada,Andrea Lopez-Lopez,María J Guerra,Jose Luis Labandeira-Garcia,Rita Valenzuela

Scientific reports 14:18974 PubMed39152142

2024

TRIM44 enhances autophagy via SQSTM1 oligomerization in response to oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqin Wang,Lin Lyu,Trung Vu,Nami McCarty

JCI insight 9: PubMed39024551

2024

PI3Kγ promotes neutrophil extracellular trap formation by noncanonical pyroptosis in abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Yacheng Xiong,Shuai Liu,Yu Liu,Jiani Zhao,Jinjian Sun,Yongqing Li,Baihong Pan,Wei Wang

Cancers 16: PubMed39001537

2024

Phosphodiesterase Inhibition to Sensitize Non-Small-Cell Lung Cancer to Pemetrexed: A Double-Edged Strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Anna V Ivanina Foureau,David M Foureau,Cody C McHale,Fei Guo,Carol J Farhangfar,Kathryn F Mileham

Cellular and molecular life sciences : CMLS 81:264 PubMed38878214

2024

Prostaglandin I signaling prevents angiotensin II-induced atrial remodeling and vulnerability to atrial fibrillation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Meng Yuan,Wenbin Cai,Weiyan Sun,Xuelian Shi,Daiqi Liu,Wenhua Song,Yingqun Yan,Tienan Chen,Qiankun Bao,Bangying Zhang,Tong Liu,Yi Zhu,Xu Zhang,Guangping Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PKA Kinase Activity Assay Kit

Functional Studies - PKA Kinase Activity Assay Kit (AB139435)

Functional Studies - PKA Kinase Activity Assay Kit (AB139435)

Functional Studies - PKA Kinase Activity Assay Kit (AB139435)

Key facts

Detection method

Sample types

Assay type

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (74)

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Primary Antibodies

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Product promise