PKA Kinase Activity Kit (ab139435) is a non-radioactive assay providing a safe, rapid and reliable method for the screening of inhibitors or activators of PKA and for quantitating the activity of PKA in purified or partially purified enzyme preparations.
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- Journal of cellular and molecular medicine 27:3706-37162023Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Chen,Bing-Rui Xiong,Shu-Yue Xian,Jing Zhang,Rui-Wen Ding,Ming Xu,Zong-Ze ZhangPubMed 37950418
- Burns & trauma 11:tkad0052023Melatonin influences the biological characteristics of keloid fibroblasts through the Erk and Smad signalling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaobin Huang,Wuguo Deng,Yunxian Dong,Zhicheng Hu,Yi Zhang,Peng Wang,Xiaoling Cao,Miao Chen,Pu Cheng,Hailin Xu,Wenkai Zhu,Bing Tang,Jiayuan ZhuPubMed 36873285
- Environmental science and pollution research international 30:47742-477542023Sublethal biochemical, behavioral, and physiological toxicity of extremely low dose of bendiocarb insecticide in Periplaneta americana (Blattodea: Blattidae).Applications:Unspecified applicationReactive species:Unspecified reactive speciesMilena Jankowska,Bartosz Augustyn,Justyna Maliszewska,Barbara Przeździecka,Dawid Kubiak,Olga Chełchowska,Jagoda Kaczorek,Dominik Knop,Kornelia Krajnik,Hanna Kletkiewicz,Jacek Kęsy,Justyna Rogalska,Maria StankiewiczPubMed 36745351
- International journal of molecular sciences 24:2023Alkaline Dilution Alters Sperm Motility in Dairy Goat by Affecting sAC/cAMP/PKA Pathway Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQifu He,Feng Gao,Shenghui Wu,Shaowen Wang,Zhiming Xu,Xuerui Xu,Tianyang Lan,Kang Zhang,Fusheng QuanPubMed 36675287
- Nature communications 13:78582022Loss of SUMO-specific protease 2 causes isolated glucocorticoid deficiency by blocking adrenal cortex zonal transdifferentiation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDamien Dufour,Typhanie Dumontet,Isabelle Sahut-Barnola,Aude Carusi,Méline Onzon,Eric Pussard,James Jr Wilmouth,Julie Olabe,Cécily Lucas,Adrien Levasseur,Christelle Damon-Soubeyrand,Jean-Christophe Pointud,Florence Roucher-Boulez,Igor Tauveron,Guillaume Bossis,Edward T Yeh,David T Breault,Pierre Val,Anne-Marie Lefrançois-Martinez,Antoine MartinezPubMed 36543805
- eLife 11:2022A remarkable adaptive paradigm of heart performance and protection emerges in response to marked cardiac-specific overexpression of ADCY8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKirill V Tarasov,Khalid Chakir,Daniel R Riordon,Alexey E Lyashkov,Ismayil Ahmet,Maria Grazia Perino,Allwin Jennifa Silvester,Jing Zhang,Mingyi Wang,Yevgeniya O Lukyanenko,Jia-Hua Qu,Miguel Calvo-Rubio Barrera,Magdalena Juhaszova,Yelena S Tarasova,Bruce Ziman,Richard Telljohann,Vikas Kumar,Mark Ranek,John Lammons,Rostislav Bychkov,Rafael de Cabo,Seungho Jun,Gizem Keceli,Ashish Gupta,Dongmei Yang,Miguel A Aon,Luigi Adamo,Christopher H Morrell,Walter Otu,Cameron Carroll,Shane Chambers,Nazareno Paolocci,Thanh Huynh,Karel Pacak,Robert Weiss,Loren Field,Steven J Sollott,Edward G LakattaPubMed 36515265
- Redox biology 56:1024322022Macrophage SAMSN1 protects against sepsis-induced acute lung injury in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanli Jiang,Chengtai Ma,Jiawei Bai,Xianjin DuPubMed 35981417
- Adipocyte 11:487-5002022Cancer cell-derived exosomal miR-425-3p induces white adipocyte atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnwen Liu,Wenxia Pan,Shutong Zhuang,Yuanzhi Tang,Haitao ZhangPubMed 35941833
- International journal of endocrinology 2022:72757652022Heat Shock Protein 70 Mediates the Protective Effect of Naringenin on High-Glucose-Induced Alterations of Endothelial Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhihan Zhang,Hui Liu,Xiang Hu,Yikang He,Lu Li,Xinyu Yang,Changhua Wang,Mingbai Hu,Shengxiang TaoPubMed 35958293
- Nature metabolism 4:739-7582022Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuijie Li,Wenyu Li,Juan Yuan,Petra Bullova,Jieyu Wu,Xuepei Zhang,Yong Liu,Monika Plescher,Javier Rodriguez,Oscar C Bedoya-Reina,Paulo R Jannig,Paula Valente-Silva,Meng Yu,Marie Arsenian Henriksson,Roman A Zubarev,Anna Smed-Sörensen,Carolyn K Suzuki,Jorge L Ruas,Johan Holmberg,Catharina Larsson,C Christofer Juhlin,Alex von Kriegsheim,Yihai Cao,Susanne SchlisioPubMed 35760869
Key facts
- Detection method
- Colorimetric
- Sample types
- Purified protein, Suspension cells, Tissue Extracts, Adherent cells
- Assay type
- Enzyme activity
- Assay time
- 4h 30m
Target data
What's included?
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PKA Kinase Activity Kit (ab139435) is a non-radioactive assay providing a safe, rapid and reliable method for the screening of inhibitors or activators of PKA and for quantitating the activity of PKA in purified or partially purified enzyme preparations.
Key facts
- Detection method
- Colorimetric
- Sample types
- Purified protein, Suspension cells, Tissue Extracts, Adherent cells
- Assay type
- Enzyme activity
- Assay time
- 4h 30m
- Assay Platform
- Microplate reader
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample | n 0 | mean - | SD - | C.V. <= 12 |
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
PKA Kinase Activity Kit (ab139435) is a non-radioactive assay providing a safe, rapid and reliable method for the screening of inhibitors or activators of PKA and for quantitating the activity of PKA in purified or partially purified enzyme preparations. This kit is based on a solid phase enzyme-linked immuno-absorbent assay (ELISA) that utilizes a specific synthetic peptide as a substrate for PKA and a polyclonal antibody that recognizes the phosphorylated form of the substrate. The assay is designed for the analysis of PKA activity in the solution phase. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols.
The kit offers the following advantages:
1. Safe - non-radioactive measurement of kinase activity
2. Flexible - kinetic and end-point options available
3. Fast - results in < 4.5 hours
4. Efficient - only 30 μl diluted sample needed per well
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein kinase A (PKA) also known as cAMP-dependent protein kinase plays an important role in cellular signaling. It is composed of a regulatory and a catalytic subunit having a molecular mass around 150 kDa. PKA is expressed widely in tissues such as the heart brain and skeletal muscle. The activation of PKA occurs when cyclic adenosine monophosphate (cAMP) binds to its regulatory subunit leading to the release of the catalytic subunit which then phosphorylates various target proteins.
- Biological function summary
PKA acts as a significant regulator of gene expression and cellular metabolism. It functions within the PKA signaling complex which includes A-kinase anchoring proteins (AKAPs) that localize PKA to specific areas in the cell aiding in precise function. PKA modulates processes such as lipolysis glycogen metabolism and transcription. Through these activities it contributes to cellular responses to hormonal changes by phosphorylating key substrates.
- Pathways
PKA activation integrates into the cAMP signaling pathway an essential regulatory pathway influencing a broad range of physiological responses. This pathway connects PKA with the downstream effectors such as CREB and other transcription factors. Moreover PKA is involved in the MAPK pathway where it interacts with proteins like MEK and ERK orchestrating responses to growth factors. These pathways demonstrate PKA’s role in integrating extracellular signals into cellular actions.
- Associated diseases and disorders
Dysregulation of PKA activity associates with conditions like Cushing's syndrome and cardiac hypertrophy. In Cushing's syndrome mutations affecting the regulatory subunit of PKA can lead to unregulated hormone activity. Cardiac hypertrophy involves altered PKA pathway signaling affecting heart muscle growth and function. PKA interacts with proteins such as phospholamban in cardiac disorders impacting calcium handling and heart contractility.
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3 product images
Functional Studies - PKA Kinase Activity Assay Kit (ab139435)
Titration of positive control (active PKA), background signal subtracted (duplicates; +/- SD).
Functional Studies - PKA Kinase Activity Assay Kit (ab139435)
Titration of cAMP Protein Kinase (Recombinant human cAMP Protein Kinase Catalytic subunit ab56268), background signal subtracted (duplicates; +/- SD).
Functional Studies - PKA Kinase Activity Assay Kit (ab139435)
1e7 3T3-L1 cells were treated for 15 minutes with 1 μM dexamethasone (Dexamethasone, anti-inflammatory glucocorticoid ab120743) and 1 μg/mL insulin (Recombinant human Insulin protein (Active) ab123768) with the addition of 50 μM forskolin (Forskolin, Adenylyl cyclase activator ab120058), 0.5 mM IBMX (IBMX, Non-specific cAMP and cGMP inhibitor ab120840) or 0.1 mM 8-CPT-cAMP (8-(4-Chlorophenylthio)adenosine 3',5'-cyclic monophosphate (8-CPT-cAMP), Cell permeable cAMP analog. ab120424). Cell extracts were prepared according to the protocol. Lysates were diluted 50-fold, and tested in duplicates (+/-SD).
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