AB139437

PKC Kinase Activity Assay Kit

Name: PKC Kinase Activity Assay Kit (ab139437) | Abcam
Brand: Abcam Limited
SKU: AB139437

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(50 Publications)

4 Images

Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

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Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

1.5 x e7 THP-1 cells were incubated with 100 nM GF109203X (GF; ab144264) or Ro31-8220 mesylate (Ro; ab120374) for 30 minutes prior to activation with 10 μg x mL^-1 PMA (Sigma) for 4 hours. Control cells were left without inhibitors or PMA. Cells were lysed in 1 mL of lysis buffer, and 30 μL were tested for PKC activity (duplicates; +/- SD).

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Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

Signal from active PKC with background signal subtracted (duplicates; +/- SD).

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Lab

Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

Titration of ab139437 (duplicates; +/- SD).

Schematic Diagram - PKC Kinase Activity Assay Kit (AB139437)

Schematic Diagram

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Schematic Diagram - PKC Kinase Activity Assay Kit (AB139437)

schematic diagram of the assay.

Fitzpatrick, Daniel P. (2020) "A Mechanistic Study on the Non-genotoxic Carcinogenicity of the Food Contaminant Semicarbazide," Sure-J : Science Undergraduate Research Journal : Vol. 2 : Iss. 1, Article 2.
Available at : https : //arrow.tudublin.ie/sure_j/vol2/iss1/2

This image is courtesy of Fitzpatrick, Daniel P. (2020) "A Mechanistic Study on the Non-genotoxic Carcinogenicity of the Food Contaminant Semicarbazide," Sure-J: Science Undergraduate Research Journal: Vol. 2: Iss. 1, Article 2

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Suspension cells, Tissue Extracts, Adherent cells

Assay type

Enzyme activity

Assay time

4h 30m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

PKC Kinase Activity Assay Kit (ab139437) is a non-radioactive assay providing a safe, rapid and reliable method for the screening of inhibitors or activators of PKC and for quantitating the activity of PKC in purified or partially purified enzyme preparations. This kit is based on a solid phase enzyme-linked immuno-absorbent assay (ELISA) that utilizes a specific synthetic peptide as a substrate for PKC and a polyclonal antibody that recognizes the phosphorylated form of the substrate.

PKA activity assay principle
In this assay, a synthetic peptide is used as PKC specific substrate. The substrate, which has pre-coated on the wells on the microplate well provided, is phosphorylated by the PKC present in the sample after addition of ATP.

The phosphorylated substrate is recognized by a polyclonal antibody that recognizes only the phospho-substrate. The phospho-specific antibody is subsequently recognized a HRPconjugated secondary antibody.

The assay is finally developed with TMB substrate and color develops in proportion to the PKC activityexisting in the sample. The color development is stopped with acid stop solution and the intensity of the color is measured in a microplate reader at OD 450 nm.

The assay is designed for the analysis of PKC activity in the solution phase. For the measurement of PKC in partially purified, purified, or crude enzyme preparations from any species.

The kit offers the following advantages:
1. Safe - non-radioactive measurement of kinase activity.
2. Flexible - kinetic and end-point options available.
3. Fast - results in < 4.5 hours.
4. Efficient - only 30 μl diluted sample needed per well.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab139437-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer", "number":"AB139437-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"PKC Phosphospecific Substrate Antibody", "number":"AB139437-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody Dilution Buffer", "number":"AB139437-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Anti-Rabbit IgG: HRP Conjugate", "number":"AB139437-CMP02", "productcode":"" }, { "size":"1 x 2 mg", "name":"ATP", "number":"AB139437-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"PKC Substrate Microtiter Plate", "number":"AB139437-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB139437-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution 2", "number":"AB139437-CMP07", "productcode":"" }, { "size":"1 x 30 µL", "name":"Active PKC", "number":"AB139437-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"Kinase Assay Dilution Buffer", "number":"AB139437-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein kinase C (PKC) comprises a family of serine/threonine kinases with several isoforms such as PKC alpha beta and gamma among others. PKC enzymes play key roles in intracellular signaling particularly in mediating responses to growth factors hormones and other signals. PKC exhibits a molecular weight generally ranging around 77 to 97 kDa depending on the isoform. These enzymes show expression in many tissues but have high concentrations in the brain heart and lung. PKC activation often involves translocation to cellular membranes which is important for their signaling roles.

Biological function summary

PKC influences various cellular processes such as cell proliferation differentiation apoptosis and immune responses. It acts within larger protein complexes serving as a modulator of cellular functions. The activation of PKC leads to its association with different scaffolding proteins impacting a wide array of cell activities. PKC notably affects the function of other kinases and transcription factors demonstrating its integral role in managing cellular behavior and homeostasis.

Pathways

PKC participates in the phosphoinositide signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. In these contexts PKC activation impacts proteins like Ras and Raf kinases which play roles in cellular growth and differentiation. By modulating these pathways PKC becomes an important component in signal transduction cascades influencing cell fate decisions. Understanding the interaction between PKC and these pathways helps in elucidating its role in maintaining normal cellular activities and responses to external stimuli.

PKC is linked to cancer and cardiovascular diseases. In cancer dysregulation of PKC activity often contributes to tumor growth and progression by altering pathways that control cell division and survival. PKC abnormally interacts with proteins like Bcl-2 promoting cell survival. In cardiovascular diseases changes in PKC signaling can impact heart function contributing to conditions like heart failure through interactions with proteins such as troponin. Research on PKC and these diseases can provide insights into developing targeted therapies.

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Product protocols

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Target data

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Publications (50)

Recent publications for all applications. Explore the full list and refine your search

Animal bioscience 38:929-940 PubMed40045627

2025

Melatonin regulates rabbit sperm motility and kinematics via the MT1/PKC signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chongchong Wang,Yanyan Zhang,Shiwen He,Biao Jiang,Jinglei Huang,Hui Peng

eNeuro 12: PubMed39947902

2025

The Dual Role of A2aR in Neuroinflammation: Modulating Microglial Polarization in White Matter Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Cheng,Wenchao Cheng,Yuhan Wang,Xiuying Chen,Lan Zhang,Yi Li,Fa Shen,Dezhi Yuan,Pian Hong,Wen Huang

Stem cell research & therapy 16:1 PubMed39757190

2025

The subventricular zone neurogenic niche provides adult born functional neurons to repair cortical brain injuries in response to diterpenoid therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo Pardillo-Díaz,Patricia Pérez-García,María Ortego-Domínguez,Ricardo Gómez-Oliva,Nora Martínez-Gómez,Samuel Domínguez-García,Francisco García-Cózar,Juan Pedro Muñoz-Miranda,Rosario Hernández-Galán,Livia Carrascal,Carmen Castro,Pedro Nunez-Abades

PloS one 19:e0308299 PubMed39413095

2024

Mycobacterium avium inhibits protein kinase C and MARCKS phosphorylation in human cystic fibrosis and non-cystic fibrosis cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin J Kokesh,Niharika Bala,Yunus E Dogan,Van-Anh L Nguyen,Marcus Costa,Abdel Alli

STAR protocols 5:103208 PubMed39068659

2024

A protocol for measuring the activity of protein kinase C-delta in murine bone-marrow-derived dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Seyedeh Mahdiye Mohati,Arash Mohammadi Matak,Stephanie Makdissi,Francesca Di Cara

Redox biology 75:103239 PubMed38901102

2024

S-nitrosoglutathione reductase alleviates morphine analgesic tolerance by restricting PKCα S-nitrosation.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Yan Su,Lijin Jiao,Qianjin Liu,Xinhua Qiao,Ting Xie,Zhiyu Ma,Min Xu,Mao-Sen Ye,Lu-Xiu Yang,Chang Chen,Yong-Gang Yao

Nature communications 15:4557 PubMed38811530

2024

Glucocorticoids paradoxically promote steroid resistance in B cell acute lymphoblastic leukemia through CXCR4/PLC signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Souleymane Abdoul-Azize,Rihab Hami,Gaetan Riou,Céline Derambure,Camille Charbonnier,Jean-Pierre Vannier,Monica L Guzman,Pascale Schneider,Olivier Boyer

International journal of molecular sciences 25: PubMed38732252

2024

Amyloid Beta Leads to Decreased Acetylcholine Levels and Non-Small Cell Lung Cancer Cell Survival via a Mechanism That Involves p38 Mitogen-Activated Protein Kinase and Protein Kinase C in a p53-Dependent and -Independent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Hind Al Khashali,Ravel Ray,Ban Darweesh,Caroline Wozniak,Ben Haddad,Stuti Goel,Issah Seidu,Jeneen Khalil,Brooke Lopo,Nayrooz Murshed,Jeffrey Guthrie,Deborah Heyl,Hedeel Guy Evans

Scientific reports 14:4921 PubMed38418632

2024

The role of leptin in regulation of the soluble amyloid precursor protein α (sAPPα) levels in lung cancer cell media.

Applications

Unspecified application

Species

Unspecified reactive species

Ben Haddad,Jeneen Khalil,Hind Al Khashali,Ravel Ray,Stuti Goel,Ban Darweesh,Kai-Ling Coleman,Caroline Wozniak,Robert Ranzenberger,Brooke Lopo,Jeffrey Guthrie,Deborah Heyl,Hedeel Guy Evans

Cell reports 43:113744 PubMed38329874

2024

Peroxisome deficiency underlies failures in hepatic immune cell development and antigen presentation in a severe Zellweger disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Brendon D Parsons,Daniel Medina-Luna,Michal Scur,Marinella Pinelli,Gayani S Gamage,Rebecca A Chilvers,Yannick Hamon,Ibrahim H I Ahmed,Stéphane Savary,Andrew P Makrigiannis,Nancy E Braverman,Juan F Rodriguez-Alcazar,Eicke Latz,Tobias K Karakach,Francesca Di Cara

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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PKC Kinase Activity Assay Kit

Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

Functional Studies - PKC Kinase Activity Assay Kit (AB139437)

Schematic Diagram - PKC Kinase Activity Assay Kit (AB139437)

Key facts

Detection method

Sample types

Assay type

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (50)

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Primary Antibodies

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Product promise