Plasma Kallikrein Assay Kit (ab241042) uses the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
View Alternative Names
KLK3, KLKB1, Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein, PKK
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Functional Studies - Plasma Kallikrein Assay Kit (AB241042)
Kinetic progressive curves for different amounts of PK Enzyme.
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Functional Studies - Plasma Kallikrein Assay Kit (AB241042)
Kinetic progressive curves for different amounts of Activated Plasma Samples are shown.
- FuncS
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Functional Studies - Plasma Kallikrein Assay Kit (AB241042)
Standard curve for pNA (n = 3) was used to estimate PK activity in Normal Pooled Human Plasma (n = 3). Assays were performed according to the kit protocol.
- FuncS
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Functional Studies - Plasma Kallikrein Assay Kit (AB241042)
Standard curve for pNA.
Complementary Products
Reactivity data
Product details
Plasma Kallikrein Assay Kit (ab241042) utilizes the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader. The Plasma Kallikrein Specific Inhibitor (PKSI) selectively inhibits the ability of Plasma Kallikrein to the cleave the synthetic substrate. The kit is easy-to-use and can detect PK activity of Purified Plasma Kallikrein and EDTA and citrated treated Plasma Samples.
Other Notes
This product was previously called K997 Biovision Plasma Kallikrein Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Plasma Kallikrein 1B plays significant roles in the kinin-kallikrein system. Within this system it cleaves high-molecular-weight kininogen to release bradykinin a potent vasodilator. This enzyme is not part of a larger protein complex but operates independently to modulate various physiological responses. Plasma kallikrein's activity influences processes like pain vasodilation and inflammation making it important in homeostasis and acute-phase responses.
Pathways
Plasma Kallikrein 1B participates actively in the kallikrein-kinin and the contact activation pathways. In the kallikrein-kinin system it works alongside high-molecular-weight kininogen and factor XII which plays an important role in the intrinsic pathway of coagulation. Plasma kallikrein's enzymatic activities lead to the production of bradykinin and other peptides impacting blood pressure and inflammatory processes.
Product protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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