Plasma Kallikrein Assay Kit (ab241042) uses the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Key facts
- Detection method
Colorimetric
- Sample types
Plasma
Target data
Function
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Alternative names
Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein, PKK, KLKB1, KLK3
What's included?
Recommended products
Plasma Kallikrein Assay Kit (ab241042) uses the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein, PKK, KLKB1, KLK3
Key facts
- Detection method
Colorimetric
- Sample types
Plasma
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Plasma Kallikrein Assay Kit (ab241042) utilizes the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader. The Plasma Kallikrein Specific Inhibitor (PKSI) selectively inhibits the ability of Plasma Kallikrein to the cleave the synthetic substrate. The kit is easy-to-use and can detect PK activity of Purified Plasma Kallikrein and Plasma Samples.
This product is manufactured by BioVision, an Abcam company and was previously called K997 Plasma Kallikrein Activity Assay Kit (Colorimetric). K997-100 is the same size as the 100 test size of ab241042.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Plasma Kallikrein 1B also known as PKK or KLKB1 is a serine protease enzyme with an approximate mass of 88 kDa. It is important for the blood coagulation process. This protein is mainly expressed in the liver and circulates in the blood as the inactive precursor prekallikrein. Through cleavage prekallikrein converts into its active form plasma kallikrein. Plasma kallikrein exhibits various proteolytic activities influencing multiple substrates involved in hemostatic and inflammatory responses.
- Biological function summary
Plasma Kallikrein 1B plays significant roles in the kinin-kallikrein system. Within this system it cleaves high-molecular-weight kininogen to release bradykinin a potent vasodilator. This enzyme is not part of a larger protein complex but operates independently to modulate various physiological responses. Plasma kallikrein's activity influences processes like pain vasodilation and inflammation making it important in homeostasis and acute-phase responses.
- Pathways
Plasma Kallikrein 1B participates actively in the kallikrein-kinin and the contact activation pathways. In the kallikrein-kinin system it works alongside high-molecular-weight kininogen and factor XII which plays an important role in the intrinsic pathway of coagulation. Plasma kallikrein's enzymatic activities lead to the production of bradykinin and other peptides impacting blood pressure and inflammatory processes.
- Associated diseases and disorders
Plasma Kallikrein 1B associates strongly with hereditary angioedema and diabetic retinopathy. In hereditary angioedema its dysregulation leads to excessive bradykinin production causing episodes of swelling. In diabetic retinopathy elevated plasma kallikrein activity can exacerbate vascular complications. Plasma kallikrein's disorderly activities also connect it to other proteins like C1 inhibitor in these pathological conditions highlighting its importance in clinical studies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Plasma Kallikrein Assay Kit (ab241042)
Kinetic progressive curves for different amounts of PK Enzyme.
Functional Studies - Plasma Kallikrein Assay Kit (ab241042)
Kinetic progressive curves for different amounts of Activated Plasma Samples are shown.
Functional Studies - Plasma Kallikrein Assay Kit (ab241042)
Standard curve for pNA.
Functional Studies - Plasma Kallikrein Assay Kit (ab241042)
Standard curve for pNA (n = 3) was used to estimate PK activity in Normal Pooled Human Plasma (n = 3). Assays were performed according to the kit protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com