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AB284937

Plasma Membrane Isolation Kit

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(2 Publications)

Plasma Membrane Isolation Kit ab284937 provides a proprietary set of reagents and buffers designed to enable optimized extraction and isolation of intact plasma membrane from mammalian tissues and cells.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Plasma Membrane Isolation Kit (AB284937)
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Supplier Data

Functional Studies - Plasma Membrane Isolation Kit (AB284937)

OrgFrontier™ Plasma Membrane Isolation Kit.

Cholesterol content by fraction, assayed using Cholesterol/ Cholesteryl Ester Assay Kit - Quantitation (ab65359). Cholesterol is highly enriched in the plasma membrane (approximately 22% of the total lipid content of the plasma membrane), but is found in much lower concentration (2%-6% of total lipids) in other organelle membranes.

Key facts

Sample types

Cell culture media, Tissue Culture Media

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Product details

This Plasma Membrane Isolation Kit (ab284937) provides a proprietary set of reagents and buffers designed to enable optimized extraction and isolation of intact plasma membrane from mammalian tissues and cells.

The protocol requires an ultracentrifuge to be performed and can be completed in a few hours.

Other Notes
This product was previously called K414 Biovision OrgFrontier Plasma Membrane Isolation Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "10Test": { "sellingSize": "10 Test", "publicAssetCode":"ab284937-10Test", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Homogenization Buffer", "number":"AB284937-CMP03", "productcode":"" }, { "size":"1 x 100 mL", "name":"Gradient Dilution Buffer II", "number":"AB284937-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"Protease Inhibitor Cocktail (EDTA-free)", "number":"AB284937-CMP01", "productcode":"" }, { "size":"1 x 85 mL", "name":"Density Gradient Media II", "number":"AB284937-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadr5703 PubMed40333988

2025

Niemann Pick C1 mistargeting disrupts lysosomal cholesterol homeostasis contributing to neurodegeneration in a Batten disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Abhilash P Appu,Maria B Bagh,Nisha Plavelil,Avisek Mondal,Tamal Sadhukhan,Satya P Singh,Neil J Perkins,Aiyi Liu,Anil B Mukherjee

Journal of cellular and molecular medicine 28:e70228 PubMed39622780

2024

Geraniin Alleviates Mouse Laser-Induced Choroidal Neovascularisation by Inhibiting Choroidal Endothelial Cell ACE2/Ang-(1-7)/MasR/IL-10 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyi Lu,Qi Cai,Lele Li,Jiayi Gu,Yuting Zhang,Haotian Sun,Hui Su,Lei Song
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com