JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB324630

Plasmin Activity Assay Kit (Fluorometric)

Be the first to review this product! Submit a review

|

(0 Publication)

Plasmin Activity Assay Kit (Fluorometric) is based on the ability of Plasmin to proteolytically cleave a synthetic plasmin substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence microplate readers at Ex/Em = 360/450 nm. This assay kit is simple, rapid and can detect plasmin activity as low as 8.66 µU in a variety of samples.

View Alternative Names

Plasminogen

Key facts

Detection method

Fluorometric

Sample types

EDTA Plasma

Reacts with

Human

Assay type

Enzyme activity (quantitative)

Sensitivity

= 8.66 µU

Assay time

1h

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Biological Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Plasmin Activity Assay Kit (Fluorometric) is based on the ability of Plasmin to proteolytically cleave a synthetic plasmin substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence microplate readers at Ex/Em = 360/450 nm. This assay kit is simple, rapid and can detect plasmin activity as low as 8.66 µU in a variety of samples.

Plasmin (EC 3.4.21.7) is a serine protease occurring in plasma as plasminogen. Upon activation via cleavage by plasminogen activators; plasmin solubilizes fibrin clots and activates and/or degrades compounds of the coagulation and complement systems. Plasminogen-Plasmin system has also been implicated in a wide variety of physiologic and pathologic processes, including tumor growth, invasion and metastasis.

Technical Note: Normal plasma has very low constitutive activity. Plasma must be activated by pretreatment with urokinase or streptokinase.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "EDTA Plasma", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.9" }, { "reproducibilityType": "Inter", "sample": "EDTA Plasma", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.079" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab324630-1x96Tests", "assetComponentDetails": [ { "size":"1 x 400 µL", "name":"Plasmin Substrate", "number":"AB324630-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"Plasmin Assay Buffer", "number":"AB324630-CMP02", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Plasmin Dilution Buffer", "number":"AB324630-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"AMC Standard (1 mM AMC in DMSO)", "number":"AB324630-CMP05", "productcode":"" }, { "size":"1 x 15 µL", "name":"Plasmin Positive Control", "number":"AB324630-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.. (Microbial infection) ENO/enoloase from parasite P.falciparum (strain NF54) interacts with PLG present in the mosquito blood meal to promote the invasion of the mosquito midgut by the parasite ookinete (PubMed : 21949403). The catalytic active form, plasmin, is essential for the invasion of the mosquito midgut (PubMed : 21949403).. (Microbial infection) Binds to OspC on the surface of B.burgdorferi cells, possibly conferring an extracellular protease activity on the bacteria that allows it to traverse host tissue.
See full target information Plasmin
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com