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AB133113

PPAR (alpha, delta, gamma) Transcription Factor Assay Kit

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(4 Publications)

Abcam's PPAR Transcription Factor Assay Kit (ab133113) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

View Alternative Names

NR1C1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1

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Functional Studies - PPAR (alpha, delta, gamma) Transcription Factor Assay Kit (AB133113)
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Supplier Data

Functional Studies - PPAR (alpha, delta, gamma) Transcription Factor Assay Kit (AB133113)

Transcription Factor Binding Assay for PPAR delta. Increasing amounts of positive control (total lysate) are assayed for PPAR delta DNA-binding activity using ab133113.

Functional Studies - PPAR (alpha, delta, gamma) Transcription Factor Assay Kit (AB133113)
  • FuncS

Supplier Data

Functional Studies - PPAR (alpha, delta, gamma) Transcription Factor Assay Kit (AB133113)

Transcription Factor Binding Assay for PPAR alpha. Increasing amounts of positive control (total lysate) are assayed for PPAR alpha DNA-binding activity using ab133113.

Functional Studies - PPAR (alpha, delta, gamma) Transcription Factor Assay Kit (AB133113)
  • FuncS

Supplier Data

Functional Studies - PPAR (alpha, delta, gamma) Transcription Factor Assay Kit (AB133113)

Transcription Factor Binding Assay for PPAR gamma. Increasing amounts of positive control (total lysate) are assayed for PPAR gamma DNA-binding activity using ab133113.

Key facts

Detection method

Colorimetric

Sample types

Nuclear Extracts, Suspension cells, Adherent cells

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Microplate reader

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Abcam's PPAR Transcription Factor Assay Kit (ab133113) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the PPAR response element is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPAR response element. PPAR alpha, delta, or gamma are detected by addition of specific primary antibodies directed against the individual PPARs. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133113 comes with a single plate that measures all three isoforms of PPAR alpha, delta, and gamma. There are enough reagents for one-third of a plate for each isoform.

Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands. PPARs play pivotal roles in the regulation of lipid metabolism and homeostasis and are important indirect and direct regulators of cellular insulin sensitivity.

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What's included?

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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16 : 0/18 : 1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
See full target information PPARA

Additional targets

PPARD,PPARG
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Autophagy :1-15 PubMed31964216

2020

Loss of HSPA9 induces peroxisomal degradation by increasing pexophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Doo Sin Jo,So Jung Park,Ae-Kyeong Kim,Na Yeon Park,Joon Bum Kim,Ji-Eun Bae,Hyun Jun Park,Ji Hyun Shin,Jong Wook Chang,Peter K Kim,Yong-Keun Jung,Jae-Young Koh,Seong-Kyu Choe,Kyu-Sun Lee,Dong-Hyung Cho

PLoS biology 17:e3000330 PubMed31226122

2019

The interleukin-4/PPARγ signaling axis promotes oligodendrocyte differentiation and remyelination after brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxiu Zhang,Wen Zhu,Fei Xu,Xuejiao Dai,Ligen Shi,Wei Cai,Hongfeng Mu,T Kevin Hitchens,Lesley M Foley,Xiangrong Liu,Fang Yu,Jie Chen,Yejie Shi,Rehana K Leak,Yanqin Gao,Jun Chen,Xiaoming Hu

International journal of molecular sciences 20: PubMed30979019

2019

Activation of PPARs Modulates Signalling Pathways and Expression of Regulatory Genes in Osteoclasts Derived from Human CD14+ Monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Abe Kasonga,Marlena C Kruger,Magdalena Coetzee

American journal of physiology. Endocrinology and 308:E960-70 PubMed25852007

2015

ATGL-mediated triglyceride turnover and the regulation of mitochondrial capacity in skeletal muscle.

Applications

FuncS

Species

Unspecified reactive species

Ruth C R Meex,Andrew J Hoy,Rachael M Mason,Sheree D Martin,Sean L McGee,Clinton R Bruce,Matthew J Watt
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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Alternative Product
Assay Kits

AB133107

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