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AB133107

PPAR alpha Transcription Factor Assay Kit

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(12 Publications)

PPAR alpha Transcription Factor Assay Kit (ab133107) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

View Alternative Names

NR1C1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1

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Functional Studies - PPAR alpha Transcription Factor Assay Kit (AB133107)
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Supplier Data

Functional Studies - PPAR alpha Transcription Factor Assay Kit (AB133107)

Panel A : Increasing amounts of positive control (total lysate) are assayed for PPAR alpha DNA-binding activity using ab133107.
Panel B : PPAR alpha DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.

Key facts

Detection method

Colorimetric

Sample types

Nuclear Extracts, Suspension cells, Adherent cells

Reacts with

Mouse, Rat, Human

Results type

Semi-Quantitative

Assay Platform

Microplate reader

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Product details

PPAR alpha Transcription Factor Assay Kit (ab133107) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR alpha is detected by addition of specific primary antibody directed against PPAR alpha. A secondary antibody conjugated to HRP is added to provide a sensitive colorometric readout at 450 nm. PPAR alpha Transcription Factor Assay will not cross-react with PPAR gamma or PPAR delta.

Other Notes
Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.

PPAR alpha is predominantly expressed in liver, heart and kidneys. Upon activation by ligands such as free fatty acids, lipids and other small molecules, PPAR alpha forms a heterodimeric complex with RXR alpha, and this complex will bind to response elements allowing transcription of target genes involved in lipid metabolism.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16 : 0/18 : 1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
See full target information PPARA
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 17: PubMed40573109

2025

Molecular Insights into the Nociceptive Modulation by Palmitoylethanolamide and Extract: An In Vitro Study Across the Blood-Brain Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Mulè,Rebecca Galla,Sara Ferrari,Marco Invernizzi,Francesca Uberti

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2415846 PubMed40231957

2025

Asprosin-FABP5 Interaction Modulates Mitochondrial Fatty Acid Oxidation through PPARα Contributing to MASLD Development.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Yuan Yu,Min Feng,Yi Chen,Hong-Lin Jia,Qi Zhang,Ming Tong,Yan-Xi Li,Yu Zhao,Xin-Xin Liu,Shi-Feng Cao,Zheng-Kai Wang,Hou-Wei Li,Xue Liu,Yan Zhang

Nature communications 16:1237 PubMed39890801

2025

PPARα-mediated lipid metabolism reprogramming supports anti-EGFR therapy resistance in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Valentin Van den Bossche,Julie Vignau,Engy Vigneron,Isabella Rizzi,Hannah Zaryouh,An Wouters,Jérôme Ambroise,Steven Van Laere,Simon Beyaert,Raphaël Helaers,Cédric van Marcke,Lionel Mignion,Elise Y Lepicard,Bénédicte F Jordan,Céline Guilbaud,Olivier Lowyck,Hajar Dahou,Antonella Mendola,Manon Desgres,Léo Aubert,Isabelle Gerin,Guido T Bommer,Romain Boidot,Perrine Vermonden,Aurélien Warnant,Yvan Larondelle,Jean-Pascal Machiels,Olivier Feron,Sandra Schmitz,Cyril Corbet

eLife 12: PubMed38976495

2024

Sex-specific resilience of neocortex to food restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Zahid Padamsey,Danai Katsanevaki,Patricia Maeso,Manuela Rizzi,Emily E Osterweil,Nathalie L Rochefort

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2304702 PubMed38145969

2023

Induction of Fatty Acid Oxidation Underlies DNA Damage-Induced Cell Death and Ameliorates Obesity-Driven Chemoresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Sunsook Hwang,Seungyeon Yang,Kyungsoo Park,Byungjoo Kim,Minjoong Kim,Seungmin Shin,Ahyoung Yoo,Jiyun Ahn,Juneil Jang,Yeong Shin Yim,Rho H Seong,Seung Min Jeong

Gastroenterology 165:1136-1150 PubMed37541526

2023

Ceramides Increase Fatty Acid Utilization in Intestinal Progenitors to Enhance Stemness and Increase Tumor Risk.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Li,Bhagirath Chaurasia,M Mahidur Rahman,Vincent Kaddai,J Alan Maschek,Jordan A Berg,Joseph L Wilkerson,Ziad S Mahmassani,James Cox,Peng Wei,Peter J Meikle,Donald Atkinson,Liping Wang,Annelise M Poss,Mary C Playdon,Trevor S Tippetts,Esraa M Mousa,Kesara Nittayaboon,Pon Velayutham Anandh Babu,Micah J Drummond,Hans Clevers,James A Shayman,Yoshio Hirabayashi,William L Holland,Jared Rutter,Bruce A Edgar,Scott A Summers

Molecules (Basel, Switzerland) 28: PubMed36677854

2023

Concomitant Sub-Chronic Administration of Small-Size Gold Nanoparticles Aggravates Doxorubicin-Induced Liver Oxidative and Inflammatory Damage, Hyperlipidemia, and Hepatic Steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ghedeir M Alshammari,Mohamed Anwar Abdelhalim,Mohammed S Al-Ayed,Laila Naif Al-Harbi,Mohammed Abdo Yahya

Frontiers in physiology 11:615025 PubMed33408645

2020

Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, .

Applications

Unspecified application

Species

Unspecified reactive species

Alexander J Watts,Samantha M Logan,Anna Kübber-Heiss,Annika Posautz,Gabrielle Stalder,Johanna Painer,Kristina Gasch,Sylvain Giroud,Kenneth B Storey

Obesity science & practice 5:383-394 PubMed31452923

2019

Linoleic acid in diets of mice increases total endocannabinoid levels in bowel and liver: modification by dietary glucose.

Applications

Unspecified application

Species

Unspecified reactive species

S Ghosh,J F O'Connell,O D Carlson,I González-Mariscal,Y Kim,R Moaddel,P Ghosh,J M Egan

PloS one 13:e0198403 PubMed29912977

2018

Tongxinluo attenuates reperfusion injury in diabetic hearts by angiopoietin-like 4-mediated protection of endothelial barrier integrity via PPAR-α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Qi,Xiangdong Li,Yongjian Geng,Hehe Cui,Chen Jin,Peihe Wang,Yue Li,Yuejin Yang
View all publications
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