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PPAR alpha Transcription Factor Assay Kit (ab133107) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

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Functional Studies - PPAR alpha Transcription Factor Assay Kit (AB133107), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Nuclear Extracts, Suspension cells, Adherent cells
Assay type
Semi-quantitative
Reactive species
Mouse, Rat, Human

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Target data

Function

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.

Alternative names

What's included?

96 Test
Components
96-Well Plate Cover
1 x 1 Unit
Polysorbate 20
1 x 1 Vial
Transcription Factor Antibody Binding Buffer (10X)
1 x 3 mL
Transcription Factor Binding Assay Buffer (4X)
1 x 3 mL
Transcription Factor Developing Solution
1 x 12 mL
Transcription Factor Goat Anti-Rabbit HRP Conjugate
1 x 100 µL
Transcription Factor PPAR 96-Well Strip Plate
1 x 1 Unit
Transcription Factor PPAR Competitor dsDNA
1 x 1 Vial
Transcription Factor PPAR alpha Positive Control
1 x 1 Vial
Transcription Factor PPAR alpha Primary Antibody
1 x 1 Vial
Transcription Factor Reagent A
1 x 120 µL
Transcription Factor Stop Solution
1 x 12 mL
Wash Buffer Concentrate (400X)
1 x 5 mL

Recommended products

PPAR alpha Transcription Factor Assay Kit (ab133107) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

Key facts

Detection method
Colorimetric
Sample types
Nuclear Extracts, Suspension cells, Adherent cells
Assay type
Semi-quantitative
Reactive species
Mouse, Rat, Human
Assay Platform
Microplate reader

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Abcam's PPAR alpha Transcription Factor Assay Kit (ab133107) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR alpha is detected by addition of specific primary antibody directed against PPAR alpha. A secondary antibody conjugated to HRP is added to provide a sensitive colorometric readout at 450 nm. PPAR alpha Transcription Factor Assay will not cross-react with PPAR gamma or PPAR delta.

Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.

PPAR alpha is predominantly expressed in liver, heart and kidneys. Upon activation by ligands such as free fatty acids, lipids and other small molecules, PPAR alpha forms a heterodimeric complex with RXR alpha, and this complex will bind to response elements allowing transcription of target genes involved in lipid metabolism.

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1 product image

  • Functional Studies - PPAR alpha Transcription Factor Assay Kit (ab133107), expandable thumbnail

    Functional Studies - PPAR alpha Transcription Factor Assay Kit (ab133107)

    Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR alpha DNA-binding activity using ab133107.
    Panel B: PPAR alpha DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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