PPAR alpha Transcription Factor Assay Kit (ab133107) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
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- Molecules (Basel, Switzerland) 28:2023Concomitant Sub-Chronic Administration of Small-Size Gold Nanoparticles Aggravates Doxorubicin-Induced Liver Oxidative and Inflammatory Damage, Hyperlipidemia, and Hepatic Steatosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGhedeir M Alshammari,Mohamed Anwar Abdelhalim,Mohammed S Al-Ayed,Laila Naif Al-Harbi,Mohammed Abdo YahyaPubMed 36677854
- Frontiers in physiology 11:6150252020Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, .Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander J Watts et. al.PubMed 33408645
- Obesity science & practice 5:383-3942019Linoleic acid in diets of mice increases total endocannabinoid levels in bowel and liver: modification by dietary glucose.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Ghosh et. al.PubMed 31452923
- PloS one 13:e01984032018Tongxinluo attenuates reperfusion injury in diabetic hearts by angiopoietin-like 4-mediated protection of endothelial barrier integrity via PPAR-α pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKang Qi et. al.PubMed 29912977
- Nutrients 9:2017The Combination of Blueberry Juice and Probiotics Ameliorate Non-Alcoholic Steatohepatitis (NASH) by Affecting SREBP-1c/PNPLA-3 Pathway via PPAR-α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Ren et. al.PubMed 28264426
- Journal of proteome research 14:366-732014Total body exposure to low-dose ionizing radiation induces long-term alterations to the liver proteome of neonatally exposed mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMayur V Bakshi,Omid Azimzadeh,Zarko Barjaktarovic,Stefan J Kempf,Juliane Merl-Pham,Stefanie M Hauck,Sonja Buratovic,Per Eriksson,Michael J Atkinson,Soile TapioPubMed 25299163
Key facts
- Detection method
- Colorimetric
- Sample types
- Nuclear Extracts, Suspension cells, Adherent cells
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Rat, Human
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Target data
Function
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
Alternative names
NR1C1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1
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PPAR alpha Transcription Factor Assay Kit (ab133107) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
Key facts
- Detection method
- Colorimetric
- Sample types
- Nuclear Extracts, Suspension cells, Adherent cells
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Rat, Human
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's PPAR alpha Transcription Factor Assay Kit (ab133107) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR alpha is detected by addition of specific primary antibody directed against PPAR alpha. A secondary antibody conjugated to HRP is added to provide a sensitive colorometric readout at 450 nm. PPAR alpha Transcription Factor Assay will not cross-react with PPAR gamma or PPAR delta.
Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.
PPAR alpha is predominantly expressed in liver, heart and kidneys. Upon activation by ligands such as free fatty acids, lipids and other small molecules, PPAR alpha forms a heterodimeric complex with RXR alpha, and this complex will bind to response elements allowing transcription of target genes involved in lipid metabolism.
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Functional Studies - PPAR alpha Transcription Factor Assay Kit (ab133107)
Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR alpha DNA-binding activity using ab133107.
Panel B: PPAR alpha DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.
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