Abcam's PPAR delta Transcription Factor Assay Kit (ab133106) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
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- International journal of molecular sciences 23:2022Cadmium Toxicity Is Regulated by Peroxisome Proliferator-Activated Receptor δ in Human Proximal Tubular Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChikage Mori,Jin-Yong Lee,Maki Tokumoto,Masahiko SatohPubMed 35955783
- Cancer discovery 12:2414-24332022A Cancer Cell-Intrinsic GOT2-PPARδ Axis Suppresses Antitumor Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaime Abrego,Hannah Sanford-Crane,Chet Oon,Xu Xiao,Courtney B Betts,Duanchen Sun,Shanthi Nagarajan,Luis Diaz,Holly Sandborg,Sohinee Bhattacharyya,Zheng Xia,Lisa M Coussens,Peter Tontonoz,Mara H ShermanPubMed 35894778
- Frontiers in physiology 11:6150252020Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, .Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander J Watts et. al.PubMed 33408645
- International journal of molecular sciences 21:2020Stearoyl-CoA Desaturase-1 Attenuates the High Shear Force Damage Effect on Human MG63 Osteosarcoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKuo-Chin Huang et. al.PubMed 32630668
- PLoS pathogens 10:e10041672014The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia.
Key facts
- Detection method
- Colorimetric
- Sample types
- Nuclear Extracts, Suspension cells, Adherent cells
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
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Target data
Function
Ligand-activated transcription factor key mediator of energy metabolism in adipose tissues (PubMed:35675826). Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
Alternative names
NR1C2, PPARB, PPARD, Peroxisome proliferator-activated receptor delta, PPAR-delta, NUCI, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, Peroxisome proliferator-activated receptor beta, NUC1, PPAR-beta
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Abcam's PPAR delta Transcription Factor Assay Kit (ab133106) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
Key facts
- Detection method
- Colorimetric
- Sample types
- Nuclear Extracts, Suspension cells, Adherent cells
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Human
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's PPAR delta Transcription Factor Assay Kit (ab133106) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR delta is detected by addition of specific primary antibody directed against PPAR delta. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133106 detects human PPAR delta.
This assay does not cross-react with PPAR gamma 1 and PPAR gamma 2 or PPAR alpha.
Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.
PPAR delta is the least understood; however, identification of a high-affinity PPAR delta synthetic ligand suggests a potential role for this receptor in lipid metabolism and obesity. PPAR delta is ubiquitously expressed, but it is particularly abundant in tissues such as liver, intestine, kidney, abdominal adipose, and skeletal muscle.
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Functional Studies - PPAR delta Transcription Factor Assay Kit (ab133106)
Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR delta DNA-binding activity using ab133106.
Panel B: PPAR delta DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.
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