PPAR gamma Transcription Factor Assay kit ab133101 is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
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- Redox biology 62:1026932023Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijie Ma,Kaifeng Li,Wenxiang Wei,Jiaheng Zhou,Ze Li,Tuo Zhang,Yunshu Wangsun,Fei Tian,Qianqian Dong,Haifeng Zhang,Wenjuan XingPubMed 37030149
- Pharmaceuticals (Basel, Switzerland) 16:2023HMG-CoA Reductase Inhibitors Suppress Monosodium Urate-Induced NLRP3 Inflammasome Activation through Peroxisome Proliferator-Activated Receptor-γ Activation in THP-1 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeong-Kyu Kim,Jung-Yoon Choe,Ji-Won Kim,Ki-Yeun ParkPubMed 37111279
- Nature communications 13:21872022USP22 regulates lipidome accumulation by stabilizing PPARγ in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Ning,Xin Guo,Xiaolong Liu,Chang Lu,Aman Wang,Xiaolin Wang,Wen Wang,Huan Chen,Wangshu Qin,Xinyu Liu,Lina Zhou,Chi Ma,Jian Du,Zhikun Lin,Haifeng Luo,Wuxiyar Otkur,Huan Qi,Di Chen,Tian Xia,Jiwei Liu,Guang Tan,Guowang Xu,Hai-Long PiaoPubMed 35449157
- Neuroscience bulletin 37:1412-14262021Pharmacological Activation of RXR-α Promotes Hematoma Absorption via a PPAR-γ-dependent Pathway After Intracerebral Hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaoran Xu,Huaijun Chen,Shengjun Zhou,Chenjun Sun,Xiaolong Xia,Yucong Peng,Jianfeng Zhuang,Xiongjie Fu,Hanhai Zeng,Hang Zhou,Yang Cao,Qian Yu,Yin Li,Libin Hu,Guoyang Zhou,Feng Yan,Gao Chen,Jianru LiPubMed 34142331
- Journal of molecular medicine (Berlin, Germany) 99:1151-11712021LMO3 reprograms visceral adipocyte metabolism during obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriel Wagner,Anna Fenzl,Josefine Lindroos-Christensen,Elisa Einwallner,Julia Husa,Nadine Witzeneder,Sabine Rauscher,Marion Gröger,Sophia Derdak,Thomas Mohr,Hedwig Sutterlüty,Florian Klinglmüller,Silviya Wolkerstorfer,Martina Fondi,Gregor Hoermann,Lei Cao,Oswald Wagner,Florian W Kiefer,Harald Esterbauer,Martin BilbanPubMed 34018016
- Antioxidants & redox signaling 35:319-3402021Activation of Endogenous HS Biosynthesis or Supplementation with Exogenous HS Enhances Adipose Tissue Adipogenesis and Preserves Adipocyte Physiology in Humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFerran Comas,Jèssica Latorre,Francisco Ortega,María Arnoriaga Rodríguez,Matthias Kern,Aina Lluch,Wifredo Ricart,Matthias Blüher,Cecilia Gotor,Luis C Romero,José Manuel Fernández-Real,José María Moreno-NavarretePubMed 33554726
- Frontiers in physiology 11:6150252020Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, .Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander J Watts et. al.PubMed 33408645
- The EMBO journal 39:e1045792020Involvement of circulating factors in the transmission of paternal experiences through the germline.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGretchen van Steenwyk et. al.PubMed 33034389
- International journal of molecular sciences 21:2020FGF21 Protects against Aggravated Blood-Brain Barrier Disruption after Ischemic Focal Stroke in Diabetic db/db Male Mice via Cerebrovascular PPARγ Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYinghua Jiang et. al.PubMed 32012810
- Biochimica et biophysica acta. Molecular and cell 1865:1585952020Effects of peptidoglycan on the development of steatohepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeiling Jin et. al.PubMed 31953228
Key facts
- Detection method
- Colorimetric
- Sample types
- Nuclear Extracts, Suspension cells, Adherent cells
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Rat, Human
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Target data
Function
Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of BMAL1 in the blood vessels (By similarity). (Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.
Alternative names
NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, Nuclear receptor subfamily 1 group C member 3
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PPAR gamma Transcription Factor Assay kit ab133101 is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
Key facts
- Detection method
- Colorimetric
- Sample types
- Nuclear Extracts, Suspension cells, Adherent cells
- Assay type
- Semi-quantitative
- Reactive species
- Mouse, Rat, Human
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
PPAR gamma Transcription Factor Assay kit ab133101 is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA)sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96 well plate. PPARs contained in a nuclear extract bind specifically to the PPRE. PPAR gamma is detected by addition of specific primary antibody directed against PPAR gamma. A secondary antibody conjugated to HRP is added to provide a sensitive colorometric readout at 450 nm. PPAR gamma will not crossreact with PPAR delta or PPAR alpha.
Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.
PPAR gamma is primarily expressed in adipose tissue and to a lesser extent in the colon, immune system and the retina. PPAR gamma was first identified as regulator of adipogenesis, but also plays an important role in cellular differentiation, insulin sensitization , atherosclerosis and cancer.
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Functional Studies - PPAR gamma Transcription Factor Assay Kit (ab133101)
3T3-L1 cells were differentiated to adipocytes with 1 uM dexamethasone (Dexamethasone, anti-inflammatory glucocorticoid ab120743), 1 ug x mL-1 insulin (Recombinant human Insulin protein (Active) ab123768) and 0.5 mM IBMX (IBMX, Non-specific cAMP and cGMP inhibitor ab120840). From the third day, the cells were grown in normal medium with the addition of only 1 ug x mL-1 insulin. From day six, the cells were cultured in normal medium for an additional two days. 40 uL of nuclear extracts were tested in duplicates (+/- SD).
Functional Studies - PPAR gamma Transcription Factor Assay Kit (ab133101)
Different volumes of positive control with inhibitor (duplicates, +/-SD).
Functional Studies - PPAR gamma Transcription Factor Assay Kit (ab133101)
Different volumes of positive control (duplicates, +/-SD).
Functional Studies - PPAR gamma Transcription Factor Assay Kit (ab133101)
Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR gamma DNA-binding activity using ab133101.
Panel B: PPAR gamma DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.
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