Proline Assay Kit (Fluorometric)

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Proline is an imino acid with a unique cyclic structure that is important for the mechanical stability of proteins. It is often referred to as a helix breaker due to its ability to disrupt alpha helices in protein secondary structures. Proline itself has a molecular mass of approximately 115.13 Da. This amino acid is widely distributed across various tissues with high levels of expression in collagen-rich tissues such as skin and connective tissues where its structural role aids in maintaining elasticity and strength.

Biological function summary

The cyclic structure of the proline amino acid influences protein folding and stability. Proline plays an important role in the formation of turns and loops within protein structures which are critical for creating functional three-dimensional configurations. Additionally proline residues often emerge as components of enzyme active sites influencing substrate specificity and enzyme activity. Although it is not part of any particular large molecular complex its presence within numerous proteins highlights its essential role in protein architecture and function.

Pathways

Proline integrates into both the proline metabolism pathway and collagen biosynthesis. In proline metabolism it contributes to the proline cycle affecting the cellular redox state by interconverting with pyrroline-5-carboxylate. Proline is involved in the synthesis of collagen a major structural protein in mammals. Its presence is linked with enzymes such as prolyl hydroxylase and proline dehydrogenase which play pivotal roles in these pathways. These connections highlight proline’s functional significance in maintaining cellular and tissue health.

Associated diseases and disorders

The metabolism and structural properties of proline connect it to conditions such as osteogenesis imperfecta and hyperprolinemia. The altered metabolism of proline due to enzyme deficiencies like proline oxidase can lead to hyperprolinemia characterized by elevated proline levels. Meanwhile proline's role in collagen structure affects osteogenesis imperfecta a disorder marked by brittle bones. The intricate involvement of proline with proteins such as collagen emphasizes its importance in these conditions making it a target for therapeutic studies and interventions using tools like a proline assay kit or proline kit.