Propidium Iodide Flow Cytometry Kit

• Flow Cyt

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Flow Cytometry - Propidium Iodide Flow Cytometry Kit (AB139418)

Sample flow cytometry data using ab139418 on untreated HeLa cells. (A) Propidium iodide histogram with DNA content color coded. This is a typical result for untreated healthy cells : most cells are G1 stage (2N), some cells are undergoing DNA synthesis (2N-4N) and the final population is mitotic (4N). Very few cells are apoptotic (<2N) or have excess more than 4N DNA content (>4N). (B) Ungated Forward and Side-scatter plot for HeLa cells in which has been pseudo-colored based on the DNA content shown in (A). Note that cell size is proportional to DNA content. (C) Gated Forward and Side-scatter plot which excludes the small debris (lower left) and cell aggregates (upper right) that are seen in (B). This is the gate used for the Propidium Iodide histogram shown in (A).

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Functional Studies - Propidium Iodide Flow Cytometry Kit (AB139418)

The cell cycle arrest caused by CPT-CEF in a concentration-dependent manner in A549 cells. (a) Untreated, (b) 1/2 IC50 treated cells and (c) IC50 treated A549 cells. (d) The graphical representation of the trend in cell cycle arrest of A549 cells in response to CPT-CEF treatment.

Krishnan, Poorani et al., Scientific reports: vol. 7,1 10962., Fig 13, doi:10.1038/s41598-017-09140-1

• Flow Cyt

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Flow Cytometry - Propidium Iodide Flow Cytometry Kit (AB139418)

Flow cytometry. Sample analysis of an experiment using ab139418 on HeLa cells. Use flow cytometer software to establish markers on a histogram plot to quantify the percentage of cells with <2N, 2N, 2N-4N, 4N and >4N DNA content.

• Flow Cyt

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Flow Cytometry - Propidium Iodide Flow Cytometry Kit (AB139418)

Flow cytometry. Comparison of fixation and staining conditions using ab139418 on Jurkat cells. Jurkat cells were untreated (Control) or treated with Thymidine or Nocodazole for 24h. Parallel plates of cells were fixed with 66% Ethanol, 66% Methanol or 4% Paraformaldehyde; each preparation was then stained with propidium iodine with or without the addition of RNase. For each fixation condition, treatment with RNase yields tighter propidium iodide peaks. Moreover, Ethanol and Methanol fixation yield superior Propidium iodide data relative to Paraformaldehyde fixation.

• Flow Cyt

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Flow Cytometry - Propidium Iodide Flow Cytometry Kit (AB139418)

Flow Cytometry.

Sample experiment using ab139418 on HeLa cells treated with Thymidine and Nocodazole. (A) Untreated HeLa cells with the expected distribution of cells with 2N, 2N-4N and 4N DNA content. (B) HeLa cells treated with Thymidine have predominantly 2N DNA content. Thymidine inhibits DNA synthesis. (C) HeLa cells treated with Nocodazole have predominantly 4N DNA content. Nocodazole is a microtubule inhibitor that causes mitotic arrest.