Protease Activity Assay Kit

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Proteases also known as peptidases or proteinases are enzymes that catalyze the breakdown of proteins into smaller peptides or amino acids by cleaving peptide bonds. These enzymes vary in mass typically ranging from 20 to 90 kDa depending on the specific type and its functional domain. They are expressed across different tissues and cellular compartments with high concentrations in digestive organs lysosomes and blood plasma. The mechanical process of protease activity is central to many physiological processes making their precise function an important focus of study in biology.

Biological function summary

Proteases play critical roles in maintaining cellular functions including protein catabolism cell signaling and the regulation of the cell cycle. They are often components of large complexes such as the proteasome where they help degrade ubiquitinated proteins ensuring protein homeostasis. Proteases also assist in activating precursor molecules into biologically active forms involving themselves in processes like blood coagulation immune responses and apoptosis. Their activity is essential in remodeling extracellular matrix and processing bioactive molecules further influencing diverse physiological pathways.

Pathways

Proteases significantly impact both the proteolytic and the ubiquitin-proteasome pathways. Within the proteolytic pathway enzymes break down proteins into peptides and amino acids for cellular recycling and energy production. The ubiquitin-proteasome pathway involving ubiquitin-related proteins is important for regulating protein degradation and turnover impacting cellular functions and stress responses. Proteases also interact with other proteins such as kinases and phosphatases which facilitate cellular signaling cascades reflecting their participation in broader biological networks.

Associated diseases and disorders

Many proteases have associations with cancer and neurodegenerative diseases. Aberrant protease activity can lead to uncontrolled cell proliferation or faulty cell death contributing to carcinogenesis. In neurodegenerative disorders such as Alzheimer's disease improper protease activity results in the accumulation of misfolded proteins like amyloid-beta peptides disrupting neural function. Proteases also have connections with proteins such as tau and APP in Alzheimer's disease indicating their complex role in pathogenesis and providing potential targets for therapeutic interventions.